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In Mesopore Protein Digestion: A New Forthcoming Strategy in Proteomics
Laboratory of Mass Spectrometry and Proteomics, Department of Experimental and Clinical Medicine, “Magna Græcia” University of Catanzaro, Catanzaro 88100, Italy
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Received: 2 June 2011; in revised form: 7 July 2011 / Accepted: 13 July 2011 / Published: 15 July 2011
Abstract: The conventional protocols for in solution or in gel protein digestion require many steps and long reaction times. The use of trypsin immobilized onto solid supports has recently captured the attention of many research groups, because these systems can speed-up protein digestion significantly. The utilization of new materials such as mesoporous silica as supports, in which enzyme and substrate are dramatically concentrated and confined in the nanospace, offers new opportunities to reduce the complexity of proteomics workflows. An overview of the procedures for in situ proteolysis of single proteins or complex protein mixtures is reported, with a special focus on porous materials used as catalysts. The challenging efforts for designing such systems aimed at mimicking the biochemistry of living cells are reviewed. Potentials, limitations and challenges of this branch of enzyme catalysis, which we indicate as in mesopore digestion, are discussed, in relation to its suitability for high-speed and high-throughput proteomics.
Keywords: mesoporous materials; protein digestion; catalysis; mass spectrometry; proteomics; nanotechnology
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Savino, R.; Casadonte, F.; Terracciano, R. In Mesopore Protein Digestion: A New Forthcoming Strategy in Proteomics. Molecules 2011, 16, 5938-5962.
Savino R, Casadonte F, Terracciano R. In Mesopore Protein Digestion: A New Forthcoming Strategy in Proteomics. Molecules. 2011; 16(7):5938-5962.
Savino, Rocco; Casadonte, Francesca; Terracciano, Rosa. 2011. "In Mesopore Protein Digestion: A New Forthcoming Strategy in Proteomics." Molecules 16, no. 7: 5938-5962.