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Molecules 2011, 16(7), 5938-5962; doi:10.3390/molecules16075938

In Mesopore Protein Digestion: A New Forthcoming Strategy in Proteomics

Laboratory of Mass Spectrometry and Proteomics, Department of Experimental and Clinical Medicine, “Magna Græcia” University of Catanzaro, Catanzaro 88100, Italy
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Received: 2 June 2011 / Revised: 7 July 2011 / Accepted: 13 July 2011 / Published: 15 July 2011
(This article belongs to the Special Issue Catalytic Asymmetric Synthesis)
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Abstract

The conventional protocols for in solution or in gel protein digestion require many steps and long reaction times. The use of trypsin immobilized onto solid supports has recently captured the attention of many research groups, because these systems can speed-up protein digestion significantly. The utilization of new materials such as mesoporous silica as supports, in which enzyme and substrate are dramatically concentrated and confined in the nanospace, offers new opportunities to reduce the complexity of proteomics workflows. An overview of the procedures for in situ proteolysis of single proteins or complex protein mixtures is reported, with a special focus on porous materials used as catalysts. The challenging efforts for designing such systems aimed at mimicking the biochemistry of living cells are reviewed. Potentials, limitations and challenges of this branch of enzyme catalysis, which we indicate as in mesopore digestion, are discussed, in relation to its suitability for high-speed and high-throughput proteomics.
Keywords: mesoporous materials; protein digestion; catalysis; mass spectrometry; proteomics; nanotechnology mesoporous materials; protein digestion; catalysis; mass spectrometry; proteomics; nanotechnology
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Savino, R.; Casadonte, F.; Terracciano, R. In Mesopore Protein Digestion: A New Forthcoming Strategy in Proteomics. Molecules 2011, 16, 5938-5962.

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