Next Article in Journal
Synthetic Organic Electrochemistry in Ionic Liquids: The Viscosity Question
Previous Article in Journal
The Use of Response Surface Methodology to Optimize the Ultrasound-Assisted Extraction of Five Anthraquinones from Rheum palmatum L.
Previous Article in Special Issue
Opportunities Offered by Chiral η6-Arene/N-Arylsulfonyl-diamine-RuII Catalysts in the Asymmetric Transfer Hydrogenation of Ketones and Imines
Molecules 2011, 16(7), 5938-5962; doi:10.3390/molecules16075938
Review

In Mesopore Protein Digestion: A New Forthcoming Strategy in Proteomics

,  and *
Received: 2 June 2011; in revised form: 7 July 2011 / Accepted: 13 July 2011 / Published: 15 July 2011
(This article belongs to the Special Issue Catalytic Asymmetric Synthesis)
Download PDF [756 KB, uploaded 18 June 2014]
Abstract: The conventional protocols for in solution or in gel protein digestion require many steps and long reaction times. The use of trypsin immobilized onto solid supports has recently captured the attention of many research groups, because these systems can speed-up protein digestion significantly. The utilization of new materials such as mesoporous silica as supports, in which enzyme and substrate are dramatically concentrated and confined in the nanospace, offers new opportunities to reduce the complexity of proteomics workflows. An overview of the procedures for in situ proteolysis of single proteins or complex protein mixtures is reported, with a special focus on porous materials used as catalysts. The challenging efforts for designing such systems aimed at mimicking the biochemistry of living cells are reviewed. Potentials, limitations and challenges of this branch of enzyme catalysis, which we indicate as in mesopore digestion, are discussed, in relation to its suitability for high-speed and high-throughput proteomics.
Keywords: mesoporous materials; protein digestion; catalysis; mass spectrometry; proteomics; nanotechnology mesoporous materials; protein digestion; catalysis; mass spectrometry; proteomics; nanotechnology
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Export to BibTeX |
EndNote


MDPI and ACS Style

Savino, R.; Casadonte, F.; Terracciano, R. In Mesopore Protein Digestion: A New Forthcoming Strategy in Proteomics. Molecules 2011, 16, 5938-5962.

AMA Style

Savino R, Casadonte F, Terracciano R. In Mesopore Protein Digestion: A New Forthcoming Strategy in Proteomics. Molecules. 2011; 16(7):5938-5962.

Chicago/Turabian Style

Savino, Rocco; Casadonte, Francesca; Terracciano, Rosa. 2011. "In Mesopore Protein Digestion: A New Forthcoming Strategy in Proteomics." Molecules 16, no. 7: 5938-5962.


Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert