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The Use of Response Surface Methodology to Optimize the Ultrasound-Assisted Extraction of Five Anthraquinones from Rheum palmatum L.
College of Pharmacy, Hebei University, Baoding 071002, China
The Affiliated Ruikang Hospital of Guangxi Traditional Chinese Medical College, Nanning 530011, China
College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun 130118, China
Department of Chemistry and Chemical Engineering, Ankang University, Ankang 725000, China
* Authors to whom correspondence should be addressed.
Received: 1 June 2011; in revised form: 7 July 2011 / Accepted: 11 July 2011 / Published: 15 July 2011
Abstract: In this paper, ultrasound-assisted extraction (UAE) was applied to the extraction of anthraquinones (aloe-emodin, rhein, emodin, chrysophanol and physcion) from Rheum palmatum L. The five anthraquinones were quantified and analyzed by high performance liquid chromatography coupled with UV detection (HPLC-UV). The extraction solvent, extraction temperature and extraction time parameters, the three main factors for UAE, were optimized with response surface methodology (RSM) to obtain the highest extraction efficiency. The optimal conditions were the use of 84% methanol as solvent, an extraction time of 33 min and an extraction temperature of 67 °C. Under these optimal conditions, the experimental values agreed closely with the predicted values. The analysis of variance indicated a high goodness of model fit and the success of RSM method for optimizing anthraquinones extraction in Rheum palmatum L.
Keywords: ultrasound-assisted extraction; anthraquinones; Rheum palmatum L; Response surface methodology
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Cite This Article
MDPI and ACS Style
Zhao, L.-C.; Liang, J.; Li, W.; Cheng, K.-M.; Xia, X.; Deng, X.; Yang, G.-L. The Use of Response Surface Methodology to Optimize the Ultrasound-Assisted Extraction of Five Anthraquinones from Rheum palmatum L.. Molecules 2011, 16, 5928-5937.
Zhao L-C, Liang J, Li W, Cheng K-M, Xia X, Deng X, Yang G-L. The Use of Response Surface Methodology to Optimize the Ultrasound-Assisted Extraction of Five Anthraquinones from Rheum palmatum L.. Molecules. 2011; 16(7):5928-5937.
Zhao, Li-Chun; Liang, Jian; Li, Wei; Cheng, Kun-Mu; Xia, Xianghua; Deng, Xin; Yang, Geng-Liang. 2011. "The Use of Response Surface Methodology to Optimize the Ultrasound-Assisted Extraction of Five Anthraquinones from Rheum palmatum L.." Molecules 16, no. 7: 5928-5937.