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Molecules 2011, 16(5), 3713-3722; doi:10.3390/molecules16053713

Simultaneous and Rapid Determination of Main Lignans in Different Parts of Schisandra Sphenanthera by Micellar Electrokinetic Capillary Chromatography

1
School of Pharmaceutical Sciences, Jilin University, Changchun, 130021, China
2
Pharmaceutical College of Beihua University, Jilin, 132013, China
3
Life Scientific Research Center of Beihua University, Jilin, 132013, China
*
Author to whom correspondence should be addressed.
Received: 7 April 2011 / Revised: 25 April 2011 / Accepted: 26 April 2011 / Published: 3 May 2011
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Abstract

Lignans are imporant active ingredients of Schisandra sphenanthera. A micellar electrokinetic chromatography method was developed for the simultaneous determination of eight lignans – schizandrin, schisandrol B, schisantherin A, schisanhenol, anwulignan, deoxyschizandrin, schizandrin B and schizandrin C – in different parts of S. sphenanthera. The key factors for separation and determination were studied and the best analysis conditions were obtained using a background electrolyte of 10 mM phosphate-37.5 mM SDS-35% v/v acetonitrile (pH 8.0) at the separation voltage of 28 kV and detection at 214 nm, whereby the plant samples could be analyzed within 9.0 min. Analysis yielded good reproducibility (RSD between 1.19-2.28%) and good recovery (between 92.2-103.8%). The detection limits (LOD) and limit of quantification (LOQ) were within 0.4-1.2 mg/L and 1.5-4.0 mg/L. This method is promising to improve the quality control of different parts of S. sphenanthera.
Keywords: micellar electrokinetic capillary chromatography (MEKC); lignans; different plant parts; Schisandra sphenanthera   micellar electrokinetic capillary chromatography (MEKC); lignans; different plant parts; Schisandra sphenanthera  
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Yuan, G.; Liu, Y.; Li, T.; Wang, Y.; Sheng, Y.; Guan, M. Simultaneous and Rapid Determination of Main Lignans in Different Parts of Schisandra Sphenanthera by Micellar Electrokinetic Capillary Chromatography. Molecules 2011, 16, 3713-3722.

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