In Vitro Inhibitory Effects of Cyandin-3-rutinoside on Pancreatic α-Amylase and Its Combined Effect with Acarbose
Abstract
:1. Introduction
2. Results and Discussion
2.1. The IC50 values for pancreatic α-amylase
2.2. The type of inhibition of cyanidin-3-rutinoside on pancreatic α-amylase
2.3. The combined effect of cyanidin-3-rutinoside with acarbose on inhibition of pancreatic α-amylase activity in vitro
3. Experimental
3.1. Chemicals
3.2. Pancreatic α-amylase inhibition assay
3.3. Enzyme kinetics
3.4. Combined inhibitory effect of cyanidin-3-rutinoside and acarbose
3.5. Statistical analysis
4. Conclusions
Acknowledgements
References and Notes
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Concentration of cyanidin-3-rutinoside (αM) | |||||||
0.1 | 1.0 | 10.0 | 100.0 | 250.0 | 500.0 | 1000.0 | |
% Inhibition | 0.8 ± 0.1 | 23.7 ± 0.2 | 44.3 ± 0.1 | 52.1 ± 0.2 | 64.1 ± 0.3 | 68.1 ± 0.2 | 70.3 ± 0.3 |
Compounds | IC50 values (μM) | ||
---|---|---|---|
Pancreatic α-amylase | Maltase | Sucrase | |
Cyanidin-3-rutinoside | 24.4 ± 0.1 | 2,323 ± 14.8 a | 250.2 ± 8.1 a |
Acarbose | 18.1 ± 0.1 | 2.7 ± 0.1 a | 29.6 ± 3.5 a |
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Akkarachiyasit, S.; Yibchok-Anun, S.; Wacharasindhu, S.; Adisakwattana, S. In Vitro Inhibitory Effects of Cyandin-3-rutinoside on Pancreatic α-Amylase and Its Combined Effect with Acarbose. Molecules 2011, 16, 2075-2083. https://doi.org/10.3390/molecules16032075
Akkarachiyasit S, Yibchok-Anun S, Wacharasindhu S, Adisakwattana S. In Vitro Inhibitory Effects of Cyandin-3-rutinoside on Pancreatic α-Amylase and Its Combined Effect with Acarbose. Molecules. 2011; 16(3):2075-2083. https://doi.org/10.3390/molecules16032075
Chicago/Turabian StyleAkkarachiyasit, Sarinya, Sirintorn Yibchok-Anun, Sumrit Wacharasindhu, and Sirichai Adisakwattana. 2011. "In Vitro Inhibitory Effects of Cyandin-3-rutinoside on Pancreatic α-Amylase and Its Combined Effect with Acarbose" Molecules 16, no. 3: 2075-2083. https://doi.org/10.3390/molecules16032075