Molecules 2011, 16(3), 2075-2083; doi:10.3390/molecules16032075
Article

In Vitro Inhibitory Effects of Cyandin-3-rutinoside on Pancreatic α-Amylase and Its Combined Effect with Acarbose

Sarinya Akkarachiyasit 1 email, Sirintorn Yibchok-Anun 1 email, Sumrit Wacharasindhu 2 email and Sirichai Adisakwattana 3,4,* email
1 Department of Pharmacology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand 2 Natural Products Research Unit, Department of Chemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand 3 Department of Transfusion Medicine, Faculty of Allied Health Sciences, The Medical Food Research and Development Center, Chulalongkorn University, Bangkok 10330, Thailand 4 Program in Nutrition and Dietetics, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok 10330, Thailand
* Author to whom correspondence should be addressed.
Received: 10 January 2011; in revised form: 7 February 2011 / Accepted: 28 February 2011 / Published: 2 March 2011
(This article belongs to the Section Medicinal Chemistry)
PDF Full-text Download PDF Full-Text [250 KB, Updated Version, uploaded 3 March 2011 08:23 CET]
The original version is still available [250 KB, uploaded 2 March 2011 11:02 CET]
Abstract: The inhibitory activity on pancreatic α-amylase by cyanidin-3-rutinoside was examined in vitro. The IC50 value of cyanidin-3-rutinoside against pancreatic α-amylase was 24.4 ± 0.1 μM. The kinetic analysis revealed that pancreatic α-amylase was inhibited by cyanidin-3-rutinoside in a non-competitive manner. The additive inhibition of a combination of cyanidin-3-rutinoside with acarbose against pancreatic α-amylase was also found. These results provide the first evidence for the effect of cyanidin-3-rutinoside in a retarded absorption of carbohydrates by inhibition of pancreatic α-amylase which may be useful as a potential inhibitor for prevention and treatment of diabetes mellitus.
Keywords: cyanidin-3-rutinoside; pancreatic α-amylase; acarbose; additive inhibition

Article Statistics

Click here to load and display the download statistics.

Cite This Article

MDPI and ACS Style

Akkarachiyasit, S.; Yibchok-Anun, S.; Wacharasindhu, S.; Adisakwattana, S. In Vitro Inhibitory Effects of Cyandin-3-rutinoside on Pancreatic α-Amylase and Its Combined Effect with Acarbose. Molecules 2011, 16, 2075-2083.

AMA Style

Akkarachiyasit S, Yibchok-Anun S, Wacharasindhu S, Adisakwattana S. In Vitro Inhibitory Effects of Cyandin-3-rutinoside on Pancreatic α-Amylase and Its Combined Effect with Acarbose. Molecules. 2011; 16(3):2075-2083.

Chicago/Turabian Style

Akkarachiyasit, Sarinya; Yibchok-Anun, Sirintorn; Wacharasindhu, Sumrit; Adisakwattana, Sirichai. 2011. "In Vitro Inhibitory Effects of Cyandin-3-rutinoside on Pancreatic α-Amylase and Its Combined Effect with Acarbose." Molecules 16, no. 3: 2075-2083.

Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert