Next Article in Journal
New Hydrophilic/Lipophilic Tetra-α-(4-carboxyphenoxy) Phthalocyanine Zinc-Mediated Photodynamic Therapy Inhibits the Proliferation of Human Hepatocellular Carcinoma Bel-7402 Cells by Triggering Apoptosis and Arresting Cell Cycle
Previous Article in Journal
Chemical Composition, Antimicrobial and Antioxidant Activities of Essential Oils from Organically Cultivated Fennel Cultivars
Article Menu

Article Versions

Export Article

Open AccessArticle
Molecules 2011, 16(2), 1378-1388; doi:10.3390/molecules16021378

Effects of Rutin and Hesperidin and Their Al(III) and Cu(II) Complexes on in Vitro Plasma Coagulation Assays

1
Faculty of Pharmacy, University of Belgrade, Vojvode Stepe 450, 11000 Belgrade, Serbia
2
Haematological Laboratory, Institute of Oncology and Radiology of Serbia, Pasterova 14, 11000 Belgrade, Serbia
*
Author to whom correspondence should be addressed.
Received: 29 November 2010 / Revised: 20 January 2011 / Accepted: 27 January 2011 / Published: 7 February 2011
(This article belongs to the Section Molecular Diversity)
Download PDF [194 KB, uploaded 18 June 2014]

Abstract

Two flavonoids, rutin and hesperidin, were investigated in vitro for anticoagulant activity through coagulation tests: activated partial thromboplastin time (aPTT), prothrombin time (PT) and thrombin time (TT). Only an ethanolic solution of rutin at the concentration of 830 µM prolonged aPTT, while TT and PT were unaffected. In order to evaluate whether the prolongation of aPTT was due to the decrease of coagulation factors, the experiment with deficient plasma was performed, showing the effects on factors VIII and IX. Since pharmacological activity of flavonoids is believed to increase when they are coordinated with metal ions, complexes of these flavonoids with Al(III) and Cu(II) ions were also tested. The results showed that complexes significantly prolonged aPTT and had no effects on PT and TT. Assay with deficient plasma (plasma having the investigated factor at less then 1%) revealed that complexes could bind to the coagulation factors, what may lead to a non-specific inhibition and aPTT prolongation. An effort was made to correlate stability of complexes with their anticoagulant properties.
Keywords: rutin; hesperidin; flavonoid-metal complexes; anticoagulation action; aPTT; PT; TT rutin; hesperidin; flavonoid-metal complexes; anticoagulation action; aPTT; PT; TT
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Kuntić, V.; Filipović, I.; Vujić, Z. Effects of Rutin and Hesperidin and Their Al(III) and Cu(II) Complexes on in Vitro Plasma Coagulation Assays. Molecules 2011, 16, 1378-1388.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]

Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top