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Effects of Rutin and Hesperidin and Their Al(III) and Cu(II) Complexes on in Vitro Plasma Coagulation Assays
Faculty of Pharmacy, University of Belgrade, Vojvode Stepe 450, 11000 Belgrade, Serbia
Haematological Laboratory, Institute of Oncology and Radiology of Serbia, Pasterova 14, 11000 Belgrade, Serbia
* Author to whom correspondence should be addressed.
Received: 29 November 2010; in revised form: 20 January 2011 / Accepted: 27 January 2011 / Published: 7 February 2011
Abstract: Two flavonoids, rutin and hesperidin, were investigated in vitro for anticoagulant activity through coagulation tests: activated partial thromboplastin time (aPTT), prothrombin time (PT) and thrombin time (TT). Only an ethanolic solution of rutin at the concentration of 830 µM prolonged aPTT, while TT and PT were unaffected. In order to evaluate whether the prolongation of aPTT was due to the decrease of coagulation factors, the experiment with deficient plasma was performed, showing the effects on factors VIII and IX. Since pharmacological activity of flavonoids is believed to increase when they are coordinated with metal ions, complexes of these flavonoids with Al(III) and Cu(II) ions were also tested. The results showed that complexes significantly prolonged aPTT and had no effects on PT and TT. Assay with deficient plasma (plasma having the investigated factor at less then 1%) revealed that complexes could bind to the coagulation factors, what may lead to a non-specific inhibition and aPTT prolongation. An effort was made to correlate stability of complexes with their anticoagulant properties.
Keywords: rutin; hesperidin; flavonoid-metal complexes; anticoagulation action; aPTT; PT; TT
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MDPI and ACS Style
Kuntić, V.; Filipović, I.; Vujić, Z. Effects of Rutin and Hesperidin and Their Al(III) and Cu(II) Complexes on in Vitro Plasma Coagulation Assays. Molecules 2011, 16, 1378-1388.
Kuntić V, Filipović I, Vujić Z. Effects of Rutin and Hesperidin and Their Al(III) and Cu(II) Complexes on in Vitro Plasma Coagulation Assays. Molecules. 2011; 16(2):1378-1388.
Kuntić, Vesna; Filipović, Ivana; Vujić, Zorica. 2011. "Effects of Rutin and Hesperidin and Their Al(III) and Cu(II) Complexes on in Vitro Plasma Coagulation Assays." Molecules 16, no. 2: 1378-1388.