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Molecules 2011, 16(12), 9792-9818; doi:10.3390/molecules16129792
Article

Probing the Interaction of Human Serum Albumin with Norfloxacin in the Presence of High-Frequency Electromagnetic Fields: Fluorescence Spectroscopy and Circular Dichroism Investigations

1
, 2
, 1
 and 1,*
Received: 17 October 2011; in revised form: 31 October 2011 / Accepted: 4 November 2011 / Published: 25 November 2011
(This article belongs to the Section Medicinal Chemistry)
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Abstract: The present study describes an investigation by fluorescence quenching, circular dichroism and UV-visible spectroscopy of the interaction between norfloxacin (NRF) and human serum albumin (HSA) in the presence of electromagnetic fields (EMFs). The results obtained from this study indicated that NRF had a strong ability to quench HSA at λex = 280 nm. In addition, a slight blue shift occurred, which suggested that the microenvironment of the protein became more hydrophobic after addition of NRF. The interaction between the NRF and HSA, whether in the absence or presence of an EMF, was considered to be a static quenching mechanism. Moreover, synchronous fluorescence demonstrated that the microenvironment around Trp became modified. Data of HSA-NRF in the presence of EMFs between 1 Hz–1 MHz confirmed the results of quenching and blue shifts. Corresponding Stern-Volmer plots were also drawn and the resultant Ksv and kq values were compared. Moreover, the binding parameters, including the number of binding sites, the binding constant and the distance, r, between donor and acceptor, were calculated based on Förster’s non-radiative energy transfer theory. According to far and near UV-CD, the formation of the complex caused changes of the secondary and tertiary structures of HSA. The obtained results are significant for patients who are subjected to high-frequency radiation as this was found to reduce the affinity of NRF to HSA.
Keywords: HSA; norfloxacin; spectroscopy; electromagnetic field; fluorescence quenching HSA; norfloxacin; spectroscopy; electromagnetic field; fluorescence quenching
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Azimi, O.; Emami, Z.; Salari, H.; Chamani, J. Probing the Interaction of Human Serum Albumin with Norfloxacin in the Presence of High-Frequency Electromagnetic Fields: Fluorescence Spectroscopy and Circular Dichroism Investigations. Molecules 2011, 16, 9792-9818.

AMA Style

Azimi O, Emami Z, Salari H, Chamani J. Probing the Interaction of Human Serum Albumin with Norfloxacin in the Presence of High-Frequency Electromagnetic Fields: Fluorescence Spectroscopy and Circular Dichroism Investigations. Molecules. 2011; 16(12):9792-9818.

Chicago/Turabian Style

Azimi, Olga; Emami, Zahra; Salari, Hanieh; Chamani, Jamshidkhan. 2011. "Probing the Interaction of Human Serum Albumin with Norfloxacin in the Presence of High-Frequency Electromagnetic Fields: Fluorescence Spectroscopy and Circular Dichroism Investigations." Molecules 16, no. 12: 9792-9818.


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