Molecules 2011, 16(12), 9819-9826; doi:10.3390/molecules16129819
Article

Synergistic Antibacterial and Antibiotic Effects of Bisbenzylisoquinoline Alkaloids on Clinical Isolates of Methicillin-Resistant Staphylococcus Aureus (MRSA)

1,* email, 1,2email, 1email, 3email, 1, 1email and 4,* email
Received: 1 August 2011; in revised form: 11 October 2011 / Accepted: 8 November 2011 / Published: 25 November 2011
(This article belongs to the Section Metabolites)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: The antibacterial activity of two bisbenzylisoquinoline alkaloids, tetrandrine (Tet) and demethyltetrandrine (d-Tet), alone and in combination with the antibiotics ampicillin (AMP), azithromycin (AZM), cefazolin (CFZ) and levofloxacin (LEV) against 10 clinical isolates of staphylococcal chromosomal cassette mec (SCCmec) III type methicillin-resistant Staphylococcus aureus (MRSA) was studied. Susceptibility to each agent alone was tested using a broth microdilution method. The chequerboard and time-kill tests were used for the combined evaluations. The minimal inhibitory concentrations/minimal bactericidal concentrations (MICs/MBCs, μg/mL) ranges alone were 64–128/256–1,024 for both Tet and d-Tet. Significant synergies against 90% of the isolates were observed for the Tet/CFZ combination, with their MICs being reduced by 75–94% [fractional inhibitory concentration indices (FICIs) ranged from 0.188 to 0.625], respectively. An additive bactericidal result was also observed for the Tet (d-Tet)/CFZ combination in the time-kill experiments. These results demonstrated that Tet and d-Tet enhanced the in vitro inhibitory efficacy of CFZ. Their potential for combinatory therapy of patients infected with MRSA warrants further pharmacological investigation.
Keywords: anti-MRSA; synergy; bisbenzylisoquinoline alkaloid; cefazolin; FICI
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MDPI and ACS Style

Zuo, G.-Y.; Li, Y.; Wang, T.; Han, J.; Wang, G.-C.; Zhang, Y.-L.; Pan, W.-D. Synergistic Antibacterial and Antibiotic Effects of Bisbenzylisoquinoline Alkaloids on Clinical Isolates of Methicillin-Resistant Staphylococcus Aureus (MRSA). Molecules 2011, 16, 9819-9826.

AMA Style

Zuo G-Y, Li Y, Wang T, Han J, Wang G-C, Zhang Y-L, Pan W-D. Synergistic Antibacterial and Antibiotic Effects of Bisbenzylisoquinoline Alkaloids on Clinical Isolates of Methicillin-Resistant Staphylococcus Aureus (MRSA). Molecules. 2011; 16(12):9819-9826.

Chicago/Turabian Style

Zuo, Guo-Ying; Li, Yang; Wang, Tao; Han, Jun; Wang, Gen-Chun; Zhang, Yun-Ling; Pan, Wei-Dong. 2011. "Synergistic Antibacterial and Antibiotic Effects of Bisbenzylisoquinoline Alkaloids on Clinical Isolates of Methicillin-Resistant Staphylococcus Aureus (MRSA)." Molecules 16, no. 12: 9819-9826.


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