Next Article in Journal
Chemistry and Structure-Activity Relationship of the Styrylquinoline-Type HIV Integrase Inhibitors
Next Article in Special Issue
Neuroprotective Herbs and Foods from Different Traditional Medicines and Diets
Previous Article in Journal
Assay Format as a Critical Success Factor for Identification of Novel Inhibitor Chemotypes of Tissue-Nonspecific Alkaline Phosphatase from High-Throughput Screening
Previous Article in Special Issue
trans-Resveratrol as A Neuroprotectant
Article Menu

Article Versions

Export Article

Open AccessArticle
Molecules 2010, 15(5), 3038-3047; doi:10.3390/molecules15053038

MDL-28170 Has No Analgesic Effect on CCI Induced Neuropathic Pain in Mice

Department of Neurology, University of Würzburg, Josef-Schneider-Str. 11, 97080 Würzburg, Germany
Author to whom correspondence should be addressed.
Received: 22 February 2010 / Revised: 13 April 2010 / Accepted: 26 April 2010 / Published: 27 April 2010
(This article belongs to the Special Issue Neuroprotective Strategies)
Download PDF [595 KB, uploaded 18 June 2014]


The calpain inhibitor MDL-28710 blocks the early local pro-inflammatory cytokine gene expression in mice after chronic constriction nerve injury (CCI). One-hundred-thirteen wild type mice of C57Bl/6J background received CCI of the right sciatic nerve. Mechanical paw withdrawal thresholds and thermal withdrawal latencies were investigated at baseline and at 1, 3, and 7 days after CCI. Three application regimens were used for MDL-28170: a) single injection 40 min before CCI; b) serial injections of MDL-28170 40 min before and up to day three after CCI; c) sustained application via intraperitoneal osmotic pumps. The control animals received the vehicle DMSO/PEG 400. The tolerable dose of MDL-28170 for mice was 30 mg/kg body weight, higher doses were lethal within the first hours after application. Mechanical withdrawal thresholds and thermal withdrawal latencies were reduced after CCI and did not normalize after single or serial injections, nor with application of MDL-28170 via osmotic pumps. Although the calpain inhibitor MDL-28170 inhibits the early local cytokine upregulation in the sciatic nerve after CCI, pain behavior is not altered. This finding implies that local cytokine upregulation after nerve injury alone is only one factor in the induction and maintenance of neuropathic pain.
Keywords: calpain; neuropathic pain; MDL-28170; chronic constriction nerve injury (CCI) calpain; neuropathic pain; MDL-28170; chronic constriction nerve injury (CCI)
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Üçeyler, N.; Biko, L.; Sommer, C. MDL-28170 Has No Analgesic Effect on CCI Induced Neuropathic Pain in Mice. Molecules 2010, 15, 3038-3047.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top