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Molecules 2010, 15(1), 352-373; doi:10.3390/molecules15010352

Interest of Fluorescence Derivatization and Fluorescence Probe Assisted Post-column Detection of Phospholipids: A Short Review

Groupe de Chimie Analytique de Paris-Sud, Faculty of Pharmacy, University Paris-Sud, EA 4041, IFR 141, 92296 Ch√Ętenay-Malabry, France
Author to whom correspondence should be addressed.
Received: 2 December 2009 / Revised: 9 January 2010 / Accepted: 12 January 2010 / Published: 18 January 2010
(This article belongs to the Special Issue Phospholipids)
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Phospholipids are essential constituents of all living cell membranes. There are many analytical methods available for the quantitative and qualitative determination of phospholipids, but since these molecules lack chromophores, common absorbance based methods are of limited use. Beside mass spectrometry, some less specific approaches that are routinely used are evaporative light scattering detection or fluorescence, which exhibit sufficient sensitivity. Here, we focus on fluorescence, which remains an interesting way to quantify phospholipids. Two ways of detecting phospholipids by fluorescence are possible coupled with separation techniques such as thin layer chromatography (TLC), high performance liquid chromatography (HPLC) and capillary electrophoresis (CE): firstly, pre-column derivatization procedures and secondly, probe assisted post-column detection with suitable fluorescence reagents. In both cases, the common purpose is to increase the detection sensitivity. It is shown that, whereas pre-column derivatization is characterized by selectivity due to the chemical functionality of the analyte involved in the derivatization process, in supramolecular post-column derivatization, the selectivity only proceeds from the capacity of the lipid to involve supramolecular assemblies with a fluorescence probe. The aim of this review is to summarize available experiments concerning fluorescence detection of phospholipids. The interest and limitation of such detection approaches are discussed.
Keywords: phospholipids; fluorescence; derivatizing reagents; probe assisted post-column detection phospholipids; fluorescence; derivatizing reagents; probe assisted post-column detection
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Ibrahim, H.; Caudron, E.; Kasselouri, A.; Prognon, P. Interest of Fluorescence Derivatization and Fluorescence Probe Assisted Post-column Detection of Phospholipids: A Short Review. Molecules 2010, 15, 352-373.

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