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Molecules 2009, 14(7), 2363-2372; doi:10.3390/molecules14072363
Article

Salivary Aldehyde Dehydrogenase: Activity towards Aromatic Aldehydes and Comparison with Recombinant ALDH3A1

1
, 2
, 3, 3, 1 and 3,*
1 Department of Drugs Analysis, Faculty of Pharmacy, Medical University of Warsaw, 1 Banacha Street, PL-02-097, Warsaw, Poland 2 Department of Pharmaceutical Microbiology, Faculty of Pharmacy, Medical University of Warsaw, 3 Oczki Street, PL-02-007, Warsaw, Poland 3 Department of Biophysics, University of Warmia and Mazury, 4 Oczapowskiego St., PL-10-719 Olsztyn, Poland
* Author to whom correspondence should be addressed.
Received: 11 May 2009 / Revised: 15 June 2009 / Accepted: 18 June 2009 / Published: 2 July 2009
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Abstract

A series of aromatic aldehydes was examined as substrates for salivary aldehyde dehydrogenase (sALDH) and the recombinant ALDH3A1. Para-substituted benzaldehydes, cinnamic aldehyde and 2-naphthaldehydes were found to be excellent substrates, and kinetic parameters for both salivary and recombinant ALDH were nearly identical. It was demonstrated that for the fluorogenic naphthaldehydes the only produced reaction product after incubation in saliva is the carboxylate.
Keywords: saliva; aldehyde dehydrogenase; enzyme kinetics; aromatic aldehydes saliva; aldehyde dehydrogenase; enzyme kinetics; aromatic aldehydes
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Giebułtowicz, J.; Wolinowska, R.; Sztybor, A.; Pietrzak, M.; Wroczyński, P.; Wierzchowski, J. Salivary Aldehyde Dehydrogenase: Activity towards Aromatic Aldehydes and Comparison with Recombinant ALDH3A1. Molecules 2009, 14, 2363-2372.

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