Next Article in Journal
Salivary Aldehyde Dehydrogenase: Activity towards Aromatic Aldehydes and Comparison with Recombinant ALDH3A1
Previous Article in Journal
Selenium and the Methionine Sulfoxide Reductase System
Molecules 2009, 14(7), 2345-2355; doi:10.3390/molecules14072345
Article

A New Synthetic Compound, 2-OH, Enhances Interleukin-2 and Interferon-γ Gene Expression in Human Peripheral Blood Mononuclear Cells

1
,
2
,
3
,
1
,
4
,
3
,
1
 and
1,*
1 Department of Life Science, Fu-Jen University, No. 510, Chung-Cheng Rd., Hsinchuang, Taipei Hsien 242, Taiwan 2 Department of Chemistry, Fu-Jen University, No. 510, Chung-Cheng Rd., Hsinchuang, Taipei Hsien 242, Taiwan 3 Department of Nutritional Science, Fu-Jen University, No. 510, Chung-Cheng Rd., Hsinchuang, Taipei Hsien 242, Taiwan 4 School of Medicine, Fu-Jen University, No. 510, Chung-Cheng Rd., Hsinchuang, Taipei Hsien 242, Taiwan
* Author to whom correspondence should be addressed.
Received: 7 May 2009 / Revised: 17 June 2009 / Accepted: 29 June 2009 / Published: 2 July 2009
View Full-Text   |   Download PDF [260 KB, uploaded 18 June 2014]   |   Browse Figures
SciFeed

Abstract

A new synthetic compound, 6-hydroxy-2-tosylisoquinolin-1(2H)-one (2-OH), was selected for immunopharmacological activity tests. The effects of 2-OH on human peripheral blood mononuclear cell (PBMC) proliferation were determined by tritiated thymidine uptake. Compared to phytohemagglutinin (PHA; 5 μg/mL) stimulation, 2-OH significantly enhanced PBMC proliferation in a dose-dependent manner. The 50% enhancement activity (EC50) for 2-OH was 4.4±0.1 μM. In addition, effects of 2-OH on interleukin-2 (IL-2) and interferon-γ (IFN-γ) production in PBMC were determined by enzyme immunoassay. Results demonstrated that 2-OH stimulated IL-2 and IFN-γ production in PBMC. Data from reverse transcription-polymerase chain reaction (RT-PCR) and real-time PCR indicated that IL-2 and IFN-γ mRNA expression in PBMC could be induced by 2-OH. Therefore, 2-OH enhanced IL-2 and IFN-γ production in PBMC by modulation their gene expression. We suggest that 2-OH may be an immunomodulatory agent.
Keywords: PBMC; proliferation; IL-2 PBMC; proliferation; IL-2
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Share & Cite This Article

Further Mendeley | CiteULike
Export to BibTeX |
EndNote |
RIS
MDPI and ACS Style

Chou, S.-H.; Chou, S.-S.P.; Liew, Y.-F.; Leu, J.-Y.; Wang, S.-J.; Huang, R.-F.S.; Tzeng, W.-F.; Kuo, Y.-C. A New Synthetic Compound, 2-OH, Enhances Interleukin-2 and Interferon-γ Gene Expression in Human Peripheral Blood Mononuclear Cells. Molecules 2009, 14, 2345-2355.

View more citation formats

Related Articles

Article Metrics

For more information on the journal, click here

Comments

[Return to top]
Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert