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Molecules 2009, 14(7), 2345-2355; doi:10.3390/molecules14072345

A New Synthetic Compound, 2-OH, Enhances Interleukin-2 and Interferon-γ Gene Expression in Human Peripheral Blood Mononuclear Cells

1
Department of Life Science, Fu-Jen University, No. 510, Chung-Cheng Rd., Hsinchuang, Taipei Hsien 242, Taiwan
2
Department of Chemistry, Fu-Jen University, No. 510, Chung-Cheng Rd., Hsinchuang, Taipei Hsien 242, Taiwan
3
Department of Nutritional Science, Fu-Jen University, No. 510, Chung-Cheng Rd., Hsinchuang, Taipei Hsien 242, Taiwan
4
School of Medicine, Fu-Jen University, No. 510, Chung-Cheng Rd., Hsinchuang, Taipei Hsien 242, Taiwan
*
Author to whom correspondence should be addressed.
Received: 7 May 2009 / Revised: 17 June 2009 / Accepted: 29 June 2009 / Published: 2 July 2009
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Abstract

A new synthetic compound, 6-hydroxy-2-tosylisoquinolin-1(2H)-one (2-OH), was selected for immunopharmacological activity tests. The effects of 2-OH on human peripheral blood mononuclear cell (PBMC) proliferation were determined by tritiated thymidine uptake. Compared to phytohemagglutinin (PHA; 5 μg/mL) stimulation, 2-OH significantly enhanced PBMC proliferation in a dose-dependent manner. The 50% enhancement activity (EC50) for 2-OH was 4.4±0.1 μM. In addition, effects of 2-OH on interleukin-2 (IL-2) and interferon-γ (IFN-γ) production in PBMC were determined by enzyme immunoassay. Results demonstrated that 2-OH stimulated IL-2 and IFN-γ production in PBMC. Data from reverse transcription-polymerase chain reaction (RT-PCR) and real-time PCR indicated that IL-2 and IFN-γ mRNA expression in PBMC could be induced by 2-OH. Therefore, 2-OH enhanced IL-2 and IFN-γ production in PBMC by modulation their gene expression. We suggest that 2-OH may be an immunomodulatory agent.
Keywords: PBMC; proliferation; IL-2 PBMC; proliferation; IL-2
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Chou, S.-H.; Chou, S.-S.P.; Liew, Y.-F.; Leu, J.-Y.; Wang, S.-J.; Huang, R.-F.S.; Tzeng, W.-F.; Kuo, Y.-C. A New Synthetic Compound, 2-OH, Enhances Interleukin-2 and Interferon-γ Gene Expression in Human Peripheral Blood Mononuclear Cells. Molecules 2009, 14, 2345-2355.

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