Molecules 2009, 14(7), 2345-2355; doi:10.3390/molecules14072345
Article

A New Synthetic Compound, 2-OH, Enhances Interleukin-2 and Interferon-γ Gene Expression in Human Peripheral Blood Mononuclear Cells

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Received: 7 May 2009; in revised form: 17 June 2009 / Accepted: 29 June 2009 / Published: 2 July 2009
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: A new synthetic compound, 6-hydroxy-2-tosylisoquinolin-1(2H)-one (2-OH), was selected for immunopharmacological activity tests. The effects of 2-OH on human peripheral blood mononuclear cell (PBMC) proliferation were determined by tritiated thymidine uptake. Compared to phytohemagglutinin (PHA; 5 μg/mL) stimulation, 2-OH significantly enhanced PBMC proliferation in a dose-dependent manner. The 50% enhancement activity (EC50) for 2-OH was 4.4±0.1 μM. In addition, effects of 2-OH on interleukin-2 (IL-2) and interferon-γ (IFN-γ) production in PBMC were determined by enzyme immunoassay. Results demonstrated that 2-OH stimulated IL-2 and IFN-γ production in PBMC. Data from reverse transcription-polymerase chain reaction (RT-PCR) and real-time PCR indicated that IL-2 and IFN-γ mRNA expression in PBMC could be induced by 2-OH. Therefore, 2-OH enhanced IL-2 and IFN-γ production in PBMC by modulation their gene expression. We suggest that 2-OH may be an immunomodulatory agent.
Keywords: PBMC; proliferation; IL-2
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MDPI and ACS Style

Chou, S.-H.; Chou, S.-S.P.; Liew, Y.-F.; Leu, J.-Y.; Wang, S.-J.; Huang, R.-F.S.; Tzeng, W.-F.; Kuo, Y.-C. A New Synthetic Compound, 2-OH, Enhances Interleukin-2 and Interferon-γ Gene Expression in Human Peripheral Blood Mononuclear Cells. Molecules 2009, 14, 2345-2355.

AMA Style

Chou S-H, Chou S-SP, Liew Y-F, Leu J-Y, Wang S-J, Huang R-FS, Tzeng W-F, Kuo Y-C. A New Synthetic Compound, 2-OH, Enhances Interleukin-2 and Interferon-γ Gene Expression in Human Peripheral Blood Mononuclear Cells. Molecules. 2009; 14(7):2345-2355.

Chicago/Turabian Style

Chou, Shiu-Huey; Chou, Shang-Shing P.; Liew, Yih-Fong; Leu, Jyh-Yih; Wang, Su-Jane; Huang, Rwei-Fen S.; Tzeng, Woan-Fang; Kuo, Yuh-Chi. 2009. "A New Synthetic Compound, 2-OH, Enhances Interleukin-2 and Interferon-γ Gene Expression in Human Peripheral Blood Mononuclear Cells." Molecules 14, no. 7: 2345-2355.

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