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• Silva, D
• Endo, E
• Filho, B
• Nakamura, C
• Svidzinski, T
• De Souza, A
• Young, M
• Ueda-Nakamura, T
• Cortez, D
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• Silva, D
• Endo, E
• Filho, B
• Nakamura, C
• Svidzinski, T
• De Souza, A
• Young, M
• Ueda-Nakamura, T
• Cortez, D
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• Silva, D
• Endo, E
• Filho, B
• Nakamura, C
• Svidzinski, T
• De Souza, A
• Young, M
• Ueda-Nakamura, T
• Cortez, D

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Molecules 2009, 14(3), 1171-1182; doi:10.3390/molecules14031171

Article

Chemical Composition and Antimicrobial Properties of Piper ovatum Vahl

Daniel Rodrigues Silva ¹ , Eliana Harue Endo ¹ , Benedito Prado Dias Filho ^{1, 2} , Celso Vataru Nakamura ^{1, 2} , Terezinha Inez Estivaleti Svidzinski ^{1, 2} , Amanda De Souza ³ , Maria Claudia M. Young ³ , Tânia Ueda-Nakamura ^{1, 2}  and Diógenes Aparício Garcia Cortez ^{1, 4,}*

¹ Programa de Pós-graduação em Ciências Farmacêuticas, Universidade Estadual de Maringá, Maringá, Paraná, Brazil ² Departamento de Análises Clínicas, Universidade Estadual de Maringá, Maringá, Paraná, Brazil ³ Instituto de Botânica de São Paulo, São Paulo, SP, Brazil ⁴ Departamento de Farmácia e Farmacologia, Universidade Estadual de Maringá, Maringá, Paraná, Brazil

* Author to whom correspondence should be addressed.

Received: 9 February 2009; in revised form: 27 February 2009 / Accepted: 11 March 2009 / Published: 16 March 2009

Download PDF Full-Text [348 KB, uploaded 16 March 2009 11:08 CET]

Abstract: The chemical composition of the essential oil obtained from the leaves of Piper ovatum Vahl by hydrodistillation was analyzed by GC–MS. The main constituents found were δ-amorphene (16.5 %), cis-muurola-4(14),5-diene (14.29 %) and γ-muurolene (13.26%). The crude extracts and isolated compounds were screened for their antimicrobial activity. Hydroalcoholic extracts of different parts of Piper ovatum Vahl, essential oil and amides isolated from leaves were tested against Gram-positive and Gram-negative bacteria and Candida species. All extracts and amides were active against Bacillus subtilis and Candida tropicalis, including clinical strains. Essential oil was active against C. tropicalis. These amides showed an inhibitory effect on the adherence of C. tropicalis ATCC 28707 on cover glasses at 10 μg/mL, but did not show morphological alterations at the tested concentrations. Amides were identified as piperovatine and piperlonguminine, and showed MIC values of 15.6 and 31.2 μg/mL to B. subtilis and 3.9 μg/mL to C. tropicalis, and low toxic effects to Vero cells and macrophages.

Keywords: Piper ovatum Vahl; Chemical composition; Essential oil; Antimicrobial activity

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