AGO2 Co-Immunoprecipitation

The AGO2 co-immunoprecipitation method was described previously [94].

Induction of Viral Lytic Cycle in NPC Cell Lines

C666-1 cells were treated with 100 ng/mL of Gemcitabine (GEM, Eli Lilly and Company, IN, USA) and 100 ng/mL Trichostatin A (TSA, Sigma-Aldrich, Saint Louis, MO, USA) for 48 days. The induction of viral lytic cycle in C666-1 cells was confirmed by Western blot analysis using antibodies targeting early viral lytic genes, anti-EA-D (BMRF1, Millipore Corp, Billerica, MA, USA), anti-EBV transcription factor R (BRLF1, Argene, Verniolle, France) and anti-EBV-ZEBRA (Argene, Verniolle, France).
