Plasmid Constructs

The v-snoRNA1 expression vector were made by inserting the PCR fragments that contains v-snoRNA1 flanking sequence (AJ507799.2, 152761:153032) into pcDNA3.1 expression vector through *Hind III* and *Xba I* sites. The PCR products were amplified using genomic DNA from B95.8 (variant 1), C666-1 (variant 2) and one of the NPC biopsy (variant 3) as template. The sequences of the primes used for the PCR are F: GCC AAG CTT GCC CTT GCG TGT C and R: GGC TCT AGA AGG CTG GCA AAG ATC.
