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Microbiology Research
Special Issues
Veterinary Microbiology and Diagnostics
share announcement

Veterinary Microbiology and Diagnostics

A special issue of Microbiology Research (ISSN 2036-7481).

Deadline for manuscript submissions: 31 August 2024 | Viewed by 411

Special Issue Editor

Dr. Seyed Ali Ghorashi

E-Mail Website
Guest Editor
1. School of Agricultural, Environmental and Veterinary Sciences, Charles Sturt University, Wagga Wagga, NSW 2678, Australia
2. Gulbali Institute, Charles Sturt University, Wagga Wagga, NSW 2678, Australia
Interests: molecular diagnosis and genotyping of veterinary pathogens; epidemiology of infectious diseases

Special Issue Information

Dear Colleagues,

We are delighted to announce the initiation of a Special Issue for the Journal of Microbiology Research, focusing on the theme “Veterinary Microbiology and Diagnostics”. Recognising the crucial role of microbiology and diagnostics in veterinary science, we aim to present a comprehensive collection of high-quality research papers, reviews, and short communications in this specialised discipline.

This Special Issue on “Veterinary Microbiology and Diagnostics” is committed to highlighting cutting-edge developments in the field. We invite veterinary professionals, researchers, and scholars to contribute their original work, spanning a diverse array of topics including infectious diseases, genotyping of veterinary pathogens, antimicrobial resistance, cutting-edge and rapid diagnostic techniques, surveillance, monitoring of veterinary pathogens, and emerging trends. This initiative serves as a collaborative platform, fostering cooperation and facilitating the exchange of knowledge among professionals and researchers deeply engaged in veterinary microbiology. By addressing critical aspects of the field, from molecular characterisations to innovative diagnostic tools and beyond, we aim to create a comprehensive collection that advances our understanding and applications of veterinary microbiology and diagnostics. Authors are encouraged to contribute their expertise, driving the dialogue forward and enhancing the collective knowledge base in this field. Join us in this effort to shape the future of veterinary microbiology and diagnostics through impactful research and collaborative exploration.

Dr. Seyed Ali Ghorashi
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Microbiology Research is an international peer-reviewed open access quarterly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 1600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • infectious diseases
  • antimicrobial resistance
  • diagnostic techniques
  • genotyping
  • emerging trends
  • zoonotic diseases
  • vaccination
  • pathogen genomics
  • molecular diagnostics
  • One Health
  • rapid diagnostics
  • veterinary pathogens
  • wildlife microbiology

Published Papers (1 paper)

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Research

16 pages, 4806 KiB  
Article
A Quadruplex Reverse Transcription Quantitative Polymerase Chain Reaction for Detecting Canine Coronavirus, Canine Rotavirus, Canine Parvovirus, and Canine Distemper Virus
by Yandi Shi, Feng Long, Kaichuang Shi, Mengyi He, Yuwen Shi, Shuping Feng, Yanwen Yin, Xiankai Wei and Zongqiang Li
Microbiol. Res. 2024, 15(2), 746-761; https://doi.org/10.3390/microbiolres15020049 - 10 May 2024
Viewed by 223
Abstract
Background: Canine coronavirus (CCoV), canine rotavirus (CRV), canine parvovirus (CPV), and canine distemper virus (CDV) cause gastroenteritis in dogs, and co-infections of these pathogens are common in China. In particular, CCoV and CRV are confirmed to have important zoonotic potential and cause public [...] Read more.
Background: Canine coronavirus (CCoV), canine rotavirus (CRV), canine parvovirus (CPV), and canine distemper virus (CDV) cause gastroenteritis in dogs, and co-infections of these pathogens are common in China. In particular, CCoV and CRV are confirmed to have important zoonotic potential and cause public health issues. It is difficult to diagnose these diseases based only on clinical manifestations and pathological damage. Methods: In this study, four pairs of specific primers and probes targeting the CCoV M, CRV VP7, CPV VP2, and CDV N genes were designed. The reaction conditions, including the primer and probe concentrations, annealing temperatures, and reaction cycles, were optimized for the development of a quadruplex RT-qPCR for the detection of CCoV, CRV, CPV, and CDV. The assay was used to test 1028 clinical samples to validate its application. Results: A quadruplex RT-qPCR was successfully established for the differential detection of CCoV, CRV, CPV, and CDV, with good specificity, high sensitivity, and excellent repeatability. The assay could specifically detect CCoV, CRV, CPV, and CDV without cross-reactivity with the other canine viruses tested. It showed high sensitivity with limits of detection (LOD) of 1.1 × 102 copies/reaction for all four plasmid constructs. It showed excellent repeatability, with 0.05–0.90% intra-assay variation and 0.02–0.94% inter-assay variation. The 1028 clinical samples were tested using the quadruplex RT-qPCR and a reported reference RT-qPCR. The positivity rates of CCoV, CRV, CPV, and CDV were 9.53%, 0.97%, 25.68%, and 5.06% using the developed assay, and 9.05%, 0.88%, 25.68%, and 4.86% using the reference assay, with agreements higher than 99.32%. Conclusion: The results indicated that a rapid and accurate quadruplex RT-qPCR was developed for the detection and differentiation of CCoV, CRV, CPV, and CDV. Full article
(This article belongs to the Special Issue Veterinary Microbiology and Diagnostics)
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