Special Issue "Transgenic Technology: Benefits or Dangers?"
A special issue of Genes (ISSN 2073-4425).
Deadline for manuscript submissions: closed (30 June 2012)
Prof. Dr. Franz Theuring
Institute of Pharmacology, Charite – University Medicine Berlin, Hessische Street 3-4, 10117 Berlin, Germany
Phone: +49 30 450525005
Fax: +49 30 450525936
Interests: transgenic technologies; proteomics (2 DE - MS); cardiovascular diseases; vasoactive molecules; functionality of tight junctions; Alzheimer's Disease; Parkinson's Disease; tumorigenesis with focus on breast cancer
Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. Papers will be published continuously (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.
Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are refereed through a peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Genes is an international peer-reviewed Open Access quarterly journal published by MDPI.
Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 300 CHF (Swiss Francs). English correction and/or formatting fees of 250 CHF (Swiss Francs) will be charged in certain cases for those articles accepted for publication that require extensive additional formatting and/or English corrections.
Genes 2012, 3(3), 481-491; doi:10.3390/genes3030481
Received: 2 July 2012; in revised form: 24 July 2012 / Accepted: 26 July 2012 / Published: 30 July 2012| Download PDF Full-text (119 KB) | Download XML Full-text
Article: Assessment of Fecundity and Germ Line Transmission in Two Transgenic Pig Lines Produced by Sleeping Beauty Transposition
Genes 2012, 3(4), 615-633; doi:10.3390/genes3040615
Received: 9 August 2012; in revised form: 10 September 2012 / Accepted: 14 September 2012 / Published: 12 October 2012| Download PDF Full-text (751 KB) | Download XML Full-text
The below list represents only planned manuscripts. Some of these manuscripts have not been received by the Editorial Office yet. Papers submitted to MDPI journals are subject to peer-review.
Type of Paper: Article
Title: Generation of Genetic Vertebrate Models by Zinc-finger and TAL Nucleases in one-cell Embryos
Author: Ralf Kuehn
Affiliation: Institute for Developmental Genetics, Bld 35/8005, Helmholtz Center Munich, German Research Center for Environmental Health (GmbH), Ingolstaedter Landstr. 1, 85764 Munich, Germany
Abstract: Gene targeting in embryonic stem (ES) cells is routinely applied to modify the mouse genome and established the mouse as the most commonly used genetic animal model (1). Direct genome editing by zinc-finger nucleases (ZFN) in one-cell embryos has been recently established as an alternative mutagenesis approach in mice, rats, rabbits and zebrafish (2-9). Such nucleases are designed to induce double-strand breaks (DSBs) at preselected genomic target sites (10-13). DSBs targeted to coding exons frequently undergo sequence deletions leading to gene knockout or allow the insertion (knock-in) of DNA sequences from gene targeting vectors via homologous recombination (HR). As recently reported for rats and zebrafish the limited availability of ZFNs is overcome by the use of modular TAL-based nucleases that enable to address any genomic target site (24, 25). This technology opens further prospects for unrestrcted modelling of human disease alleles in model organisms.
Type of Paper: Article
Title: Assessment of Fecundity and Germline Transmission in two Venus Transgenic Pig Lines Produced by Sleeping Beauty Transposition
Authors: W.Garrels 1, S.Holler 1, N. Cleve 1, H.Niemann 1, Z.Ivics 2 and W.A.Kues 1
Affiliations: 1 Friedrich-Loeffler-Institut, Institut für Nutztiergenetik, Germany; E-Mail: firstname.lastname@example.org
2 Paul-Ehrlich-Institut, Germany
Abstract: Recently, we described a method for producing transgenic pigs using a non-autonomous Sleeping Beauty transposon system. The founder animals showed ubiquitous expression of the Venus cassette in almost all cell types. To assess whether expression of the reporter fluorophore affects animal welfare and fecundity, we analyzed reproductive parameters of two founder boars. Molecular analysis of ejaculated sperm cells suggested 3 independent (monomeric) integrations of the transgene in both boars. To test germline transmission of the three monomeric integration sites, wild-type sows were artificially inseminated. The offspring were nursed to sexual maturity and two lines were established. A clear segregation of the transgenic trait following the Mendelian rules could be seen in the F1 and F2 offspring. The results show that SB-catalysed transposition is a promising approach for stable genetic modification into permissive loci in the pig genome.
Last update: 17 May 2012