Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
4.2
4.6
Journals
Bioengineering
Special Issues
Tissue Engineering for Regenerative Dentistry
share announcement

Tissue Engineering for Regenerative Dentistry

A special issue of Bioengineering (ISSN 2306-5354). This special issue belongs to the section "Regenerative Engineering".

Deadline for manuscript submissions: 30 June 2024 | Viewed by 2757

Special Issue Editors

Prof. Dr. Shahram Ghanaati

E-Mail Website
Guest Editor
Frankfurt Oral Regenerative Medicine, Clinic for Maxillofacial and Plastic Surgery, Johann Wolfgang Goethe University, 60596 Frankfurt Am Main, Germany
Interests: biomaterial research; blood concentrates; regenerative and reconstructive medicine; bone and tissue regeneration; oncology; open healing, patient specific implants; tumor surgery; implantology; tissue engineering; clinical research; translational research
Special Issues, Collections and Topics in MDPI journals
Dr. Carlos Fernando Mourão

E-Mail Website
Guest Editor
Department of Periodontology, Dental Research Administration, Tufts University School of Dental Medicine, Boston, MA 02111, USA
Interests: membranes/barries; blood-derived growth factors; guide bone regeneration; soft tissue regeneration; biomaterials; regenerative medicine; drug delivery systems
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

The field of dentistry is constantly progressing and there is great promise in using autologous blood concentrates, stem cells, and biomaterials to advance tissue regeneration.

Autologous blood concentrates have the ability to improve wound healing and tissue regrowth, particularly in oral and maxillofacial surgery. This is due to their composition of growth factors and cytokines. Stem cells have potent regenerative capabilities and can mobilize the body's own reparative mechanisms for tissue restoration.

Biomaterials, such as bioactive ceramics, polymers, and composites, can fortify these biological resources and mimic the natural extracellular matrix. They promote cell attachment, growth, and differentiation, thereby laying the foundation for successful integration into the human body.

The purpose of this Special Issue is to showcase the potential of this powerful combination for tissue engineering in dentistry. The goal is to highlight innovative research developments in bioengineering and tissue regeneration. Ultimately, dentistry can shift from traditional treatments to a more regenerative model, which will enhance the future of dental care and improve the quality of life for patients.

Prof. Dr. Shahram Ghanaati
Prof. Dr. Carlos Fernando Mourão
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Bioengineering is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Published Papers (3 papers)

Download All Papers
Order results
Result details
Show export options expand_more Show export options expand_less
Select all
Export citation of selected articles as:

Research

11 pages, 7515 KiB  
Article
Human versus Rat PRF on Collagen Membranes: A Pilot Study of Mineralization in Rat Calvaria Defect Model
by Karol Ali Apaza Alccayhuaman, Patrick Heimel, Stefan Tangl, Stefan Lettner, Carina Kampleitner, Layla Panahipour, Ulrike Kuchler and Reinhard Gruber
Bioengineering 2024, 11(5), 414; https://doi.org/10.3390/bioengineering11050414 - 23 Apr 2024
Viewed by 362
Abstract
Platelet-rich fibrin, the coagulated plasma fraction of blood, is commonly used to support natural healing in clinical applications. The rat calvaria defect is a standardized model to study bone regeneration. It remains, however, unclear if the rat calvaria defect is appropriate to investigate [...] Read more.
Platelet-rich fibrin, the coagulated plasma fraction of blood, is commonly used to support natural healing in clinical applications. The rat calvaria defect is a standardized model to study bone regeneration. It remains, however, unclear if the rat calvaria defect is appropriate to investigate the impact of human PRF (Platelet-Rich Fibrin) on bone regeneration. To this end, we soaked Bio-Gide® collagen membranes in human or rat liquid concentrated PRF before placing them onto 5 mm calvarial defects in Sprague Dawley rats. Three weeks later, histology and micro-computed tomography (μCT) were performed. We observed that the collagen membranes soaked with rat PRF show the characteristic features of new bone and areas of mineralized collagen matrix, indicated by a median mineralized volume of 1.5 mm3 (range: 0.9; 5.3 mm3). Histology revealed new bone growing underneath the membrane and hybrid bone where collagen fibers are embedded in the new bone. Moreover, areas of passive mineralization were observed. The collagen membranes soaked with human PRF, however, were devoid of histological features of new bone formation in the center of the defect; only occasionally, new bone formed at the defect margins. Human PRF (h-PRF) caused a median bone volume of 0.9 mm3 (range: 0.3–3.3 mm3), which was significantly lower than what was observed with rat PRF (r-PRF), with a BV median of 1.2 mm3 (range: 0.3–5.9 mm3). Our findings indicate that the rat calvaria defect model is suitable for assessing the effects of rat PRF on bone formation, but caution is warranted when extrapolating conclusions regarding the efficacy of human PRF. Full article
(This article belongs to the Special Issue Tissue Engineering for Regenerative Dentistry)
►▼ Show Figures

Graphical abstract

18 pages, 7997 KiB  
Article
Three Milliliters of Peripheral Blood Is Sufficient for Preparing Liquid Platelet-Rich Fibrin (PRF): An In Vitro Study
by Sarah Al-Maawi, Eva Dohle, Robert Sader and Shahram Ghanaati
Bioengineering 2024, 11(3), 253; https://doi.org/10.3390/bioengineering11030253 - 04 Mar 2024
Viewed by 721
Abstract
Platelet-rich fibrin (PRF) has assumed an important role in supporting tissue regeneration in different fields. To date, the standard protocol for liquid PRF requires at least 10 mL of peripheral blood. The present study aimed to analyze the composition, growth factor release, and [...] Read more.
Platelet-rich fibrin (PRF) has assumed an important role in supporting tissue regeneration in different fields. To date, the standard protocol for liquid PRF requires at least 10 mL of peripheral blood. The present study aimed to analyze the composition, growth factor release, and effects on the cell proliferation of PRF samples produced using 3 mL vs. 10 mL of peripheral blood in vitro. Peripheral venous blood from six healthy donors was used to prepare liquid PRF using either 3 mL or 10 mL tubes. Three different centrifugation protocols were used according to the low-speed centrifugation concept. The cellular distribution was evaluated using immunohistology and automated cell count. ELISA was used to determine the release of different growth factors (EGF, TGF-β1, and PDGF) and interleukin 8 at different time points. Primary human osteoblasts (pOBs) were cultivated for 7 days using PRF-conditioned media acquired from either 3 mL or 10 mL of peripheral blood. The results showed that 3 mL of peripheral blood is sufficient to produce a liquid PRF concentrate similar to that acquired when using 10 mL blood. The concentrations of platelets and leukocytes were comparable regardless of the initial blood volume (3 mL vs. 10 mL). Similarly, the release of growth factors (EGF, TGF-β1, and PDGF) and interleukin 8 was often comparable in both groups over 7 days. The cultivation of pOBs using PRF-conditioned media showed a similar proliferation rate regardless of the initial blood volume. This proliferation rate was also similar to that of pOBs treated with 20% FBS-conditioned media. These findings validated the use of 3 mL of peripheral blood to generate liquid PRF matrices according to the low-speed centrifugation concept, which may open new application fields for research purposes such as in vivo experiments and clinical applications such as pediatric surgery. Full article
(This article belongs to the Special Issue Tissue Engineering for Regenerative Dentistry)
►▼ Show Figures

Graphical abstract

12 pages, 2209 KiB  
Article
Platelet-Rich Fibrin-Conditioned Medium as an Alternative to Fetal Bovine Serum Promotes Osteogenesis of Human Dental Pulp Stem Cells
by Ayano Hatori, Daiki Yamakawa, Sarah Al-Maawi, Eva Dohle, Jin Chikira, Yasuyuki Fujii, Megumu Miki, Robert Sader, Daichi Chikazu, Shahram Ghanaati and Yoko Kawase-Koga
Bioengineering 2023, 10(10), 1196; https://doi.org/10.3390/bioengineering10101196 - 14 Oct 2023
Cited by 1 | Viewed by 1272
Abstract
Human dental pulp stem cells (DPSCs) exhibit multilineage differentiation capabilities and superior clonogenic and proliferative properties. However, the use of animal-derived components such as FBS raises concerns regarding the clinical application of stem-cell-based therapies. Platelet-rich fibrin (PRF) derived from human blood is rich [...] Read more.
Human dental pulp stem cells (DPSCs) exhibit multilineage differentiation capabilities and superior clonogenic and proliferative properties. However, the use of animal-derived components such as FBS raises concerns regarding the clinical application of stem-cell-based therapies. Platelet-rich fibrin (PRF) derived from human blood is rich in fibrin, platelets, and growth factors and acts as a bioactive scaffold for grafting with biomaterials. In this study, we assessed the efficacy of PRF-conditioned medium (CM) in promoting DPSCs proliferation and osteogenic differentiation compared with the standard culture medium supplemented with FBS. A comparison of DPSCs cultured in FBS and PRF-CM revealed no differences in characteristics or morphology. However, cells cultured with PRF-CM exhibited inferior proliferation rates and cell numbers during passage in comparison with those cultured with FBS. In contrast, DPSCs cultured in PRF-CM showed significantly higher levels of calcification, and RT-PCR confirmed that the gene expression levels of markers associated with osteoblast differentiation were significantly increased. The PRF-CM approach offers a convenient, straightforward, and advantageous method for culturing DPSCs, without relying on animal-derived components. In summary, this study introduces a novel application of PRF-CM for enhancing the osteogenesis of DPSCs, which provides an alternative to FBS culture medium and addresses concerns associated with the use of animal-derived components in clinical settings. Full article
(This article belongs to the Special Issue Tissue Engineering for Regenerative Dentistry)
►▼ Show Figures

Figure 1

Show export options expand_more Show export options expand_less
Select all
Export citation of selected articles as:
 
Displaying articles 1-3
Back to TopTop