Sequential Contribution of Parenchymal and Neural Stem Cell-Derived Oligodendrocyte Precursor Cells toward Remyelination
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animals
2.2. Tissue Processing and Immunohistochemistry
2.3. Cell Quantification
2.4. Statistical Analysis
3. Results
3.1. Evolution of LPC-Induced Demyelinated Lesion
3.2. Contribution of Local OPCs to Remyelinating Oligodendrocytes
3.3. Contribution of SVZ-Derived Cells to Remyelinating Oligodendrocytes
Distribution and Phenotype of YFP+ Cells in Tg(Nes-creERT2);gt(ROSA)26Sortm1(EYFP) Mice
3.4. Response of Nestin+ SVZ Cells to Acute Demyelination
3.5. Transient Migration of NSC-Derived Neuroblasts into Corpus Callosum after Demyelination
4. Discussion
4.1. Oligodendroglial Fate of Local Parenchymal OPCs and NSCs in the SVZ
4.2. Temporal and Regional Determinants of Mobilizing Local OPCs and SVZ Cells
4.3. Plasticity of Neuronal and Oligodendrocyte Lineages in the SVZ
4.4. Mechanisms that Could Affect Oligodendrogliogenesis from the SVZ
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Antibody | Host Species | Source | Dilution |
---|---|---|---|
Dcx | Rabbit | Cell Signaling Technology (Danvers, MA, USA) | 1:300 |
NG2 | Rabbit | EMD Millipore (Burlington, MA, USA) | 1:500 |
Pdgfra | Goat | R&D Systems (Minneapolis, MN, USA) | 1:1000 |
CC1 (Quaking 7) | Mouse | EMD Millipore (Burlington, MA, USA) | 1:100 |
MBP, smi99 antibody | Mouse | Covance (Princeton, NJ, USA) | 1:3000 |
Smi32 | Mouse | Covance (Princeton, NJ, USA) | 1:1000 |
GFP | Chick | Aves Labs (Tigard, OR, USA) | 1:1000 |
Olig2 | Mouse | EMD Millipore (Burlington, MA, USA) | 1:1000 |
Olig2 | Rabbit | Novus Biologicals (Littleton, CO, USA) | 1:1000 |
PSA-NCAM, 12E3 antibody | Mouse | Dr. Tatsunori Seki (Tokyo Medical University, Tokyo, Japan) | 1:1000 |
GFAP | Rabbit | DAKO-Agilent (Santa Clara, CA, USA) | 1:2000 |
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Serwanski, D.R.; Rasmussen, A.L.; Brunquell, C.B.; Perkins, S.S.; Nishiyama, A. Sequential Contribution of Parenchymal and Neural Stem Cell-Derived Oligodendrocyte Precursor Cells toward Remyelination. Neuroglia 2018, 1, 91-105. https://doi.org/10.3390/neuroglia1010008
Serwanski DR, Rasmussen AL, Brunquell CB, Perkins SS, Nishiyama A. Sequential Contribution of Parenchymal and Neural Stem Cell-Derived Oligodendrocyte Precursor Cells toward Remyelination. Neuroglia. 2018; 1(1):91-105. https://doi.org/10.3390/neuroglia1010008
Chicago/Turabian StyleSerwanski, David R., Andrew L. Rasmussen, Christopher B. Brunquell, Scott S. Perkins, and Akiko Nishiyama. 2018. "Sequential Contribution of Parenchymal and Neural Stem Cell-Derived Oligodendrocyte Precursor Cells toward Remyelination" Neuroglia 1, no. 1: 91-105. https://doi.org/10.3390/neuroglia1010008