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Neuroglia 2018, 1(1), 106-125; https://doi.org/10.3390/neuroglia1010009 (registering DOI)

Action Potential Firing Induces Sodium Transients in Macroglial Cells of the Mouse Corpus Callosum

Institute of Neurobiology, Faculty of Mathematics and Natural Sciences, Heinrich Heine University Düsseldorf, Universitätsstrasse 1, D-40225 Düsseldorf, Germany
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Received: 14 June 2018 / Revised: 26 June 2018 / Accepted: 27 June 2018 / Published: 3 July 2018
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Abstract

Recent work has established that glutamatergic synaptic activity induces transient sodium elevations in grey matter astrocytes by stimulating glutamate transporter 1 (GLT-1) and glutamate-aspartate transporter (GLAST). Glial sodium transients have diverse functional consequences but are largely unexplored in white matter. Here, we employed ratiometric imaging to analyse sodium signalling in macroglial cells of mouse corpus callosum. Electrical stimulation resulted in robust sodium transients in astrocytes, oligodendrocytes and NG2 glia, which were blocked by tetrodotoxin, demonstrating their dependence on axonal action potentials (APs). Action potential-induced sodium increases were strongly reduced by combined inhibition of ionotropic glutamate receptors and glutamate transporters, indicating that they are related to release of glutamate. While AMPA receptors were involved in sodium influx into all cell types, oligodendrocytes and NG2 glia showed an additional contribution of NMDA receptors. The transporter subtypes GLT-1 and GLAST were detected at the protein level and contributed to glutamate-induced glial sodium signals, indicating that both are functionally relevant for glutamate clearance in corpus callosum. In summary, our results demonstrate that white matter macroglial cells experience sodium influx through ionotropic glutamate receptors and glutamate uptake upon AP generation. Activity-induced glial sodium signalling may thus contribute to the communication between active axons and macroglial cells. View Full-Text
Keywords: astrocyte; oligodendrocyte; NG2 cell; SBFI; glutamate; GLT-1; GLAST astrocyte; oligodendrocyte; NG2 cell; SBFI; glutamate; GLT-1; GLAST
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Moshrefi-Ravasdjani, B.; Ziemens, D.; Pape, N.; Färfers, M.; Rose, C.R. Action Potential Firing Induces Sodium Transients in Macroglial Cells of the Mouse Corpus Callosum. Neuroglia 2018, 1, 106-125.

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