Catechol-Containing Hydroxylated Biomimetic 4-Thiaflavanes as Inhibitors of Amyloid Aggregation
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Preparation of 4-Thiaflavane Derivatives
2.3. Preparation of Hen-Egg White Lysozyme for Aggregation
2.4. Aggregation Conditions
2.5. Thioflavin T Assay
2.6. Atomic Force Microscopy
2.7. Cell Growth and Citotoxicity Assay
3. Results
3.1. 4-Thiaflavane Derivatives Obstacle/Impair Amyloid Aggregation Kinetics
3.2. 4-Thiaflavane Derivatives Do Not Compete with Thioflavin T
3.3. Polyhydroxylated 4-Thiaflavanes Inhibit the Formation of Amyloid Fibrils
3.4. 4-Thiaflavane Derivatives Do Not Prevent Toxicity Induced by Early Amyloid Aggregates
4. Discussion
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Ramazzotti, M.; Paoli, P.; Tiribilli, B.; Viglianisi, C.; Menichetti, S.; Degl’innocenti, D. Catechol-Containing Hydroxylated Biomimetic 4-Thiaflavanes as Inhibitors of Amyloid Aggregation. Biomimetics 2017, 2, 6. https://doi.org/10.3390/biomimetics2020006
Ramazzotti M, Paoli P, Tiribilli B, Viglianisi C, Menichetti S, Degl’innocenti D. Catechol-Containing Hydroxylated Biomimetic 4-Thiaflavanes as Inhibitors of Amyloid Aggregation. Biomimetics. 2017; 2(2):6. https://doi.org/10.3390/biomimetics2020006
Chicago/Turabian StyleRamazzotti, Matteo, Paolo Paoli, Bruno Tiribilli, Caterina Viglianisi, Stefano Menichetti, and Donatella Degl’innocenti. 2017. "Catechol-Containing Hydroxylated Biomimetic 4-Thiaflavanes as Inhibitors of Amyloid Aggregation" Biomimetics 2, no. 2: 6. https://doi.org/10.3390/biomimetics2020006