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Fermentation 2016, 2(3), 15; doi:10.3390/fermentation2030015

Modelling and Optimization of Operational Setpoint Parameters for Maximum Fermentative Biohydrogen Production Using Box-Behnken Design

Sustainable Energy & Environment Research Unit, School of Chemical and Metallurgical Engineering, Faculty of Engineering and the Built Environment, University of the Witwatersrand, Private Bag 3, Wits, Johannesburg 2050, South Africa
Academic Editors: Thaddeus Ezeji and Badal C. Saha
Received: 3 June 2016 / Revised: 10 July 2016 / Accepted: 15 July 2016 / Published: 20 July 2016
(This article belongs to the Special Issue Biofuels and Biochemicals Production)
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Abstract

Fermentative biohydrogen production has been flagged as a future alternative energy source due to its various socio-economical benefits. Currently, its production is hindered by the low yield. In this work, modelling and optimization of fermentative biohydrogen producing operational setpoint conditions was carried out. A box-behnken design was used to generate twenty-nine batch experiments. The experimental data were used to produce a quadratic polynomial model which was subjected to analysis of variance (ANOVA) to evaluate its statistical significance. The quadratic polynomial model had a coefficient of determination (R2) of 0.7895. The optimum setpoint obtained were potato-waste concentration 39.56 g/L, pH 5.56, temperature 37.87 °C, and fermentation time 82.58 h, predicting a biohydrogen production response of 537.5 mL H2/g TVS. A validation experiment gave 603.5 mL H2/g TVS resulting to a 12% increase. The R2 was above 0.7 implying the model was adequate to navigate the optimization space. Therefore, these findings demonstrated the feasibility of conducting optimized biohydrogen fermentation processes using response surface methodology. View Full-Text
Keywords: biohydrogen production; modelling and optimization; box-behnken design biohydrogen production; modelling and optimization; box-behnken design
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Sekoai, P.T. Modelling and Optimization of Operational Setpoint Parameters for Maximum Fermentative Biohydrogen Production Using Box-Behnken Design. Fermentation 2016, 2, 15.

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