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Fermentation 2016, 2(1), 6; doi:10.3390/fermentation2010006

Simultaneous Determination of Sugars, Carboxylates, Alcohols and Aldehydes from Fermentations by High Performance Liquid Chromatography

1,2,†
,
3,4,†
,
3,4
and
1,2,*
1
Centre for Microbial Electrochemical Systems (CEMES), The University of Queensland, Brisbane QLD 4072, Australia
2
Advanced Water Management Centre (AWMC), The University of Queensland, Brisbane QLD 4072, Australia
3
Australian Institute for Bioengineering and Nanotechnology (AIBN), The University of Queensland, Brisbane QLD 4072, Australia
4
Metabolomics Australia (Queensland Node), The University of Queensland, Brisbane QLD 4072, Australia
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Academic Editor: Thaddeus Ezeji
Received: 29 January 2016 / Revised: 23 February 2016 / Accepted: 24 February 2016 / Published: 7 March 2016
(This article belongs to the Special Issue Biofuels and Biochemicals Production)
View Full-Text   |   Download PDF [2046 KB, uploaded 7 March 2016]   |  

Abstract

Despite the rise of ‘omics techniques for the study of biological systems, the quantitative description of phenotypes still rests to a large extent on quantitative data produced on chromatography platforms. Here, we describe an improved liquid chromatography method for the determination of sugars, carboxylates, alcohols and aldehydes in microbial fermentation samples and cell extracts. Specific emphasis is given to substrates and products currently pursued in industrial microbiology. The present method allows quantification of 21 compounds in a single run with limits of quantification between 10−7 and 10−10 mol and limits of detection between 10−9 and 10−11 mol. View Full-Text
Keywords: high performance liquid chromatography; ion-exchange chromatography; metabolite separation; fermentation product quantification high performance liquid chromatography; ion-exchange chromatography; metabolite separation; fermentation product quantification
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Lai, B.; Plan, M.R.; Hodson, M.P.; Krömer, J.O. Simultaneous Determination of Sugars, Carboxylates, Alcohols and Aldehydes from Fermentations by High Performance Liquid Chromatography. Fermentation 2016, 2, 6.

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