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Fermentation 2016, 2(1), 5; doi:10.3390/fermentation2010005

Improving Process Yield in Succinic Acid Production by Cell Recycling of Recombinant Corynebacterium glutamicum

Research Institute of Innovative Technology for the Earth, 9-2, Kizugawadai, Kizugawa, Kyoto 619-0292, Japan
Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916-5, Takayama, Ikoma, Nara 630-0101, Japan
Author to whom correspondence should be addressed.
Academic Editor: George N. Bennett
Received: 3 December 2015 / Revised: 22 February 2016 / Accepted: 26 February 2016 / Published: 4 March 2016
(This article belongs to the Special Issue Metabolic Engineering)
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Aerobically cultivated cells of Corynebacterium glutamicum produce mixed organic acids, including succinic acid (SA), from glucose when the cells are transferred to oxygen-deprived conditions. Genetic modification, including inactivation of lactate dehydrogenase and overexpression of pyruvate carboxylase, allows this microbe to be an efficient SA producer under the conditions of oxygen deprivation. High productivity and high titers can be achieved in the production process by using the genetically engineered strain of C. glutamicum under the given conditions. However, glucose consumption for cell preparation decreases process yield (defined as the quantity of SA produced divided by the total quantity of glucose used in cell preparation and SA production). In this study, we investigated cell recycle fed-batch fermentation for SA production to improve the process yield by reducing the effect of glucose consumption for cell preparation on the process yield. A genetically stable and markerless strain, harboring nine genomic copies of the pyruvate carboxylase gene, was newly constructed and used for cell recycle fermentation. During 26 reaction cycles, only 0.7% decrease in specific productivity per reaction was observed. Overall, the process yield was improved by 79% compared to that in a single fed-batch reaction without cell recycling. View Full-Text
Keywords: Corynebacterium glutamicum; oxygen deprivation; succinic acid; cell recycle reaction Corynebacterium glutamicum; oxygen deprivation; succinic acid; cell recycle reaction

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Jojima, T.; Noburyu, R.; Suda, M.; Okino, S.; Yukawa, H.; Inui, M. Improving Process Yield in Succinic Acid Production by Cell Recycling of Recombinant Corynebacterium glutamicum. Fermentation 2016, 2, 5.

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