HDAC Inhibition in Vascular Endothelial Cells Regulates the Expression of ncRNAs
Abstract
:1. Introduction
2. Results
2.1. Pharmacological Inhibitors of HDAC Activity Regulate the Expression of ncRNAs
2.2. Chromatin Modifications Drive ncRNA Gene Activation and Suppression
3. Discussion
4. Materials and Methods
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
Abbreviations
ChIP-seq | Chromatin immunoprecipitation sequencing |
ENCODE | Encyclopedia of DNA Elements |
FDA | Food and Drug Administration |
FDR | False Discovery Rate |
GSEA | Gene Set Enrichment Analysis |
H3K27ac | Histone 3 lysine 27 acetylation |
H3K27me3 | Histone 3 lysine 27 trimethylation |
H3K4me1 | Histone 3 lysine 4 monomethylation |
H3K4me3 | Histone 3 lysine 4 trimethylation |
H3K9/14ac | Histone 3 lysine 9 and 14 acetylation |
H3K9ac | Histone 3 lysine 9 acetylation |
HAECs | Human Aortic Endothelial Cells |
HAT | Histone acetyltransferase |
HDAC | Histone deacetylase |
lincRNA | long intergenic non-coding RNA |
logCPM | Log of the counts per million |
logFC | log of the fold change |
miRNA | microRNA |
ncRNA | non-coding RNA |
NIH | National Institute of Health |
ORF | Open Reading Frame |
RNA-seq | RNA sequencing |
SAHA | Suberanilohydroxamic acid |
TFBS | Transcription Factor Binding Sites |
TSA | Trichostatin A |
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Rafehi, H.; El-Osta, A. HDAC Inhibition in Vascular Endothelial Cells Regulates the Expression of ncRNAs. Non-Coding RNA 2016, 2, 4. https://doi.org/10.3390/ncrna2020004
Rafehi H, El-Osta A. HDAC Inhibition in Vascular Endothelial Cells Regulates the Expression of ncRNAs. Non-Coding RNA. 2016; 2(2):4. https://doi.org/10.3390/ncrna2020004
Chicago/Turabian StyleRafehi, Haloom, and Assam El-Osta. 2016. "HDAC Inhibition in Vascular Endothelial Cells Regulates the Expression of ncRNAs" Non-Coding RNA 2, no. 2: 4. https://doi.org/10.3390/ncrna2020004