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Non-Coding RNA 2015, 1(3), 222-245; doi:10.3390/ncrna1030222

miRNA–mRNA Conflux Regulating Immunity and Oxidative Stress Pathways in the Midgut of Blood-Fed Anopheles stephensi

1
Insect Resistance Group, International Centre for Genetic Engineering and Biotechnology, New Delhi 110067, India
2
National Institute of Malaria Research, Dwarka, New Delhi-110077, India
*
Authors to whom correspondence should be addressed.
Academic Editor: George A. Calin
Received: 15 August 2015 / Revised: 2 November 2015 / Accepted: 11 November 2015 / Published: 19 November 2015
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Abstract

Blood feeding in Anopheles stephensi initiates a cascade of events that modulate several physiological functions in the mosquito. The midgut epithelium activates several of its molecules, most important among these being microRNAs, which regulate some of the physiological changes by targeting diverse mRNAs. The present study was conducted to identify and evaluate interactions between targets of eight miRNAs that are regulated on blood feeding. Identified from our previous study, we show these eight miRNAs exhibited distinct tissue specific expression. Targets of these miRNAs were predicted using computational approaches involving bioinformatics, co-expression analysis of the transcriptome and miRNome of blood-fed An. stephensi midgut. Using degradome sequencing, we identified some cleaved mRNAs of these microRNAs and, by using antagomiR knockdown technology to repress the miRNAs, the targets were validated in an An. stephensi cell line and in An. stephensi mosquitoes. In-depth analysis of predicted and identified targets revealed that the regulated miRNAs modulate well-characterized molecules that are involved in combating oxidative stress and immunity pathways through a dynamic miRNA:mRNA network. Our study is the first to identify miRNA:mRNA interactomes that play important role in maintaining redox homeostasis during blood feeding in the midgut of An. stephensi. View Full-Text
Keywords: Anopheles; Blood feeding; miRNA; Targets; Degradome; Co-Express; Lock nucleic acid; Oxidative stress Anopheles; Blood feeding; miRNA; Targets; Degradome; Co-Express; Lock nucleic acid; Oxidative stress
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Jain, S.; Shrinet, J.; Tridibes, A.; Bhatnagar, R.K.; Sunil, S. miRNA–mRNA Conflux Regulating Immunity and Oxidative Stress Pathways in the Midgut of Blood-Fed Anopheles stephensi. Non-Coding RNA 2015, 1, 222-245.

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