Next Article in Journal
Utilization of High Performance Liquid Chromatography Coupled to Tandem Mass Spectrometry for Characterization of 8-O-methylbostrycoidin Production by Species of the Fungus Fusarium
Previous Article in Journal
Strategies to Reduce Mortality in Adult and Neonatal Candidemia in Developing Countries
Previous Article in Special Issue
Metabolic Interactions between Bacteria and Fungi in Commensal Oral Biofilms
Article Menu
Issue 3 (September) cover image

Export Article

Open AccessArticle
J. Fungi 2017, 3(3), 42; doi:10.3390/jof3030042

The Effect of Novel Heterocyclic Compounds on Cryptococcal Biofilm

Department of Clinical Microbiology and Infectious Diseases, Hadassah-Hebrew University Medical Center, Jerusalem 91120, Israel
Current address: Department of Medical Neurobiology, Institute for Medical Research Israel-Canada, Hebrew University-Hadassah Medical School, Jerusalem 91120, Israel.
*
Author to whom correspondence should be addressed.
Received: 29 May 2017 / Revised: 13 July 2017 / Accepted: 17 July 2017 / Published: 20 July 2017
(This article belongs to the Special Issue Fungal Biofilms)
View Full-Text   |   Download PDF [4136 KB, uploaded 20 July 2017]   |  

Abstract

Biofilm formation by microorganisms depends on their communication by quorum sensing, which is mediated by small diffusible signaling molecules that accumulate in the extracellular environment. During human infection, the pathogenic yeast Cryptococcus neoformans can form biofilm on medical devices, which protects the organism and increases its resistance to antifungal agents. The aim of this study was to test two novel heterocyclic compounds, S-8 (thiazolidinedione derivative, TZD) and NA-8 (succinimide derivative, SI), for their anti-biofilm activity against strains of Cryptococcus neoformans and Cryptococcus gattii. Biofilms were formed in a defined medium in 96-well polystyrene plates and 8-well micro-slides. The effect of sub-inhibitory concentrations of S-8 and NA-8 on biofilm formation was measured after 48 h by a metabolic reduction assay and by confocal laser microscopy analysis using fluorescent staining. The formation and development of cryptococcal biofilms was inhibited significantly by these compounds in concentrations below the minimum inhibitory concentration (MIC) values. These compounds may have a potential role in preventing fungal biofilm development on indwelling medical devices or even as a therapeutic measure after the establishment of biofilm. View Full-Text
Keywords: Cryptococcus; biofilm; quorum sensing; thiazolidinedione derivative; succinimide derivative; auto-inducer Cryptococcus; biofilm; quorum sensing; thiazolidinedione derivative; succinimide derivative; auto-inducer
Figures

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Korem, M.; Kagan, S.; Polacheck, I. The Effect of Novel Heterocyclic Compounds on Cryptococcal Biofilm. J. Fungi 2017, 3, 42.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
J. Fungi EISSN 2309-608X Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top