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J. Cardiovasc. Dev. Dis. 2016, 3(1), 9; doi:10.3390/jcdd3010009

TLR-4 and CD14 Genotypes and Soluble CD14: Could They Predispose to Coronary Atherosclerosis?

1
Department of Cardiothoracic Surgery, Royal Brompton and Harefield Trust, 156 Montreal House, Surrey Quays Road, London SE16 7AQ, UK
2
Department of Forensic Medicine and Toxicology, Athens Medical School, Athens 115 27, Greece
3
Department of Immunology, Onassis Cardiac Surgery Center, Athens 176 74, Greece
4
Department of Radiology, Onassis Cardiac Surgery Center, Athens 176 74, Greece
5
Department of Biology, Athens Medical School, Athens115 27, Greece
*
Author to whom correspondence should be addressed.
Academic Editor: Andy Wessels
Received: 31 December 2015 / Revised: 28 February 2016 / Accepted: 4 March 2016 / Published: 10 March 2016
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Abstract

Background: Inflammatory mechanisms are key to the pathogenesis of atherosclerosis. Functional polymorphisms of TLR-4, Asp299Gly and Thr399Ile, CD14 promoter area C260T polymorphism and plasma levels of soluble CD14 are studied in subjects with Coronary Artery Disease (CAD). Methods: DNA was obtained from 100 human paraffin-embedded aortic specimens, from cadavers with known coronary atheromatosis (Group A) and 100 blood samples from patients with CAD, as detected by cardiac Multi-Detector-row-Computed-Tomography (MDCT) (Group B). Our control group consisted of 100 healthy individuals (Group C). Genotyping was performed by Restriction Fragment Length Polymorphism-Polymerase Chain Reaction (RFLP-PCR). Plasma levels of sCD14 were measured with ELISA. Results: For TLR-4 Asp299Gly and Thr399Ile polymorphisms, no statistically significant differences were observed. Regarding the C260T polymorphism, frequencies of T allele were significantly higher in the control group compared to the case group (p = 0.05). The Odds Ratio (OR) showed statistically significant association of TT genotype with healthy individuals (OR 0.25, 95% Confidence Interval CI 0.10–0.62, p = 0.0017). Plasma levels of sCD14 in patients with CAD (mean value = 1.35 μg/mL) were reduced when compared to reference value. Conclusions: The studied polymorphisms ofTLR-4 showed no association with CAD. Conversely, the functional polymorphism of CD14 has a statistically significant difference in expression between healthy and affected by CAD individuals. View Full-Text
Keywords: coronary atheroscerosis; coronary artery disease; Toll-Like-Receptor 4 (TLR-4); CD14; sCD14; PCR-RFLP; ELISA coronary atheroscerosis; coronary artery disease; Toll-Like-Receptor 4 (TLR-4); CD14; sCD14; PCR-RFLP; ELISA
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Konstantinidou, M.K.; Goutas, N.; Vlachodimitropoulos, D.; Chaidaroglou, A.; Stefanou, D.; Poumpouridou, N.; Mastorakou, R.; Gazouli, M.; Kyparissopoulos, D.; Spiliopoulou, C. TLR-4 and CD14 Genotypes and Soluble CD14: Could They Predispose to Coronary Atherosclerosis? J. Cardiovasc. Dev. Dis. 2016, 3, 9.

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