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J. Cardiovasc. Dev. Dis. 2014, 1(2), 146-162; doi:10.3390/jcdd1020146

Characterization of Dermal Fibroblasts as a Cell Source for Pediatric Tissue Engineered Heart Valves

1
Department of Bioengineering, Rice University, 6100 Main Street MS-142, Houston, TX 77005, USA
2
Department of Pathology, Texas Children's Hospital, 6621 Fannin Street, Houston, TX 77030, USA
3
Department of Congenital Heart Surgery, Texas Children's Hospital, 6621 Fannin Street, Houston, TX 77030, USA
*
Author to whom correspondence should be addressed.
Received: 17 June 2014 / Revised: 29 July 2014 / Accepted: 20 August 2014 / Published: 26 August 2014
(This article belongs to the Special Issue Semilunar Valve Development and Disease)
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Abstract

There is continued debate regarding the appropriate cell type to replace valvular interstitial cells (VICs) in tissue engineered heart valves (TEHVs), particularly for pediatric patients. In this work, neonatal human dermal fibroblasts (nhDFFs) were compared to human pediatric VICs (hpVICs), based on their phenotypic and gene expression characteristics when cultured on collagen type I, fibronectin, fibrin, and tissue culture polystyrene (TCP) substrates. Similar confluency was achieved over the culture period on collagen and fibronectin between both cell types, although nhDFFs tended to reach lower confluence on collagen than on any other substrate. Morphologically, hpVICs tended to spread and form multiple extensions, while nhDFFs remained homogenously spindle-shaped on all substrates. PCR results indicated that fibroblasts did not differ significantly from VICs in gene expression when cultured on fibrin, whereas on collagen type I and fibronectin they showed increased α-SMA, xylosyltransferase I, and collagen type I expression (p < 0.05). However, protein expression of these targets, analyzed by immunocytochemistry and Western blotting, was not significantly different between cell types. These results suggest that nhDFFs express similar matrix production and remodeling genes as hpVICs, and the choice of substrate for TEHV construction can affect the growth and expression profile of nhDFFs as compared to native hpVICs. View Full-Text
Keywords: fibroblasts; pediatric; valvular interstitial cell; VIC; TEHV; extracellular matrix; substrate fibroblasts; pediatric; valvular interstitial cell; VIC; TEHV; extracellular matrix; substrate
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This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Fahrenholtz, M.M.; Liu, H.; Kearney, D.L.; Wadhwa, L.; Fraser, C.D., Jr.; Grande-Allen, K.J. Characterization of Dermal Fibroblasts as a Cell Source for Pediatric Tissue Engineered Heart Valves. J. Cardiovasc. Dev. Dis. 2014, 1, 146-162.

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J. Cardiovasc. Dev. Dis. EISSN 2308-3425 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
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