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J. Cardiovasc. Dev. Dis. 2014, 1(2), 163-176; doi:10.3390/jcdd1020163

Sox9- and Scleraxis-Cre Lineage Fate Mapping in Aortic and Mitral Valve Structures

1
Center for Cardiovascular and Pulmonary Research and The Heart Center at Nationwide Children's Hospital Research Institute, 575 Children's Drive, Research Building III, WB4239, Columbus, OH 43215, USA
2
Department of Molecular Biology and Biochemistry, Division of Basic Life Sciences, Institute of Biomedical & Health Sciences, Hiroshima University, Hiroshima 734-8553, Japan
3
Department of Pediatrics, The Ohio State University, Columbus, OH 43205, USA
*
Author to whom correspondence should be addressed.
Received: 26 August 2014 / Revised: 16 September 2014 / Accepted: 17 September 2014 / Published: 23 September 2014
(This article belongs to the Special Issue Semilunar Valve Development and Disease)
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Abstract

Heart valves are complex structures composed of a heterogeneous population of valve interstitial cells (VICs), an overlying endothelium and highly organized layers of extracellular matrix. Alterations in valve homeostasis are characteristic of dysfunction and disease, however the mechanisms that initiate and promote valve pathology are poorly understood. Advancements have been largely hindered by the limited availability of tools for gene targeting in heart valve structures during embryogenesis and after birth. We have previously shown that the transcription factors Sox9 and Scleraxis (Scx) are required for heart valve formation and in this study we describe the recombination patterns of Sox9- and Scx-Cre lines at differential time points in aortic and mitral valve structures. In ScxCre; ROSA26GFP mice, recombination is undetected in valve endothelial cells (VECs) and low in VICs during embryogenesis. However, recombination increases in VICs from post natal stages and by 4 weeks side-specific patterns are observed. Using the inducible Sox9CreERT2 system, we observe recombination in VECs and VICs in the embryo, and high levels are maintained through post natal and juvenile stages. These Cre-drivers provide the field with new tools for gene targeting in valve cell lineages during differential stages of embryonic and post natal maturation and maintenance. View Full-Text
Keywords: heart valve; endothelial; interstitial; Sox9; Scleraxis; aortic; mitral heart valve; endothelial; interstitial; Sox9; Scleraxis; aortic; mitral
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Austin, B.F.; Yoshimoto, Y.; Shukunami, C.; Lincoln, J. Sox9- and Scleraxis-Cre Lineage Fate Mapping in Aortic and Mitral Valve Structures. J. Cardiovasc. Dev. Dis. 2014, 1, 163-176.

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J. Cardiovasc. Dev. Dis. EISSN 2308-3425 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
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