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Vet. Sci. 2018, 5(1), 26; https://doi.org/10.3390/vetsci5010026

Detection and Characterisation of Anaplasma marginale and A. centrale in South Africa

1
Vectors and Vector-borne Diseases Research Programme, Department of Veterinary Tropical Diseases, University of Pretoria, Pretoria 0110, South Africa
2
Biotechnology Platform, Agricultural Research Council, Onderstepoort, Pretoria 0110, South Africa
3
Research and Scientific Services Department, National Zoological Gardens of South Africa; Pretoria 0001, South Africa
4
Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164, USA
*
Author to whom correspondence should be addressed.
Received: 31 January 2018 / Revised: 27 February 2018 / Accepted: 28 February 2018 / Published: 3 March 2018
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Abstract

Bovine anaplasmosis is endemic in South Africa and it has a negative economic impact on cattle farming. An improved understanding of Anaplasma marginale and Anaplasma marginale variety centrale (A. centrale) transmission, together with improved tools for pathogen detection and characterisation, are required to inform best management practices. Direct detection methods currently in use for A. marginale and A. centrale in South Africa are light microscopic examination of tissue and organ smears, conventional, nested, and quantitative real-time polymerase chain reaction (qPCR) assays, and a reverse line blot hybridisation assay. Of these, qPCR is the most sensitive for detection of A. marginale and A. centrale in South Africa. Serological assays also feature in routine diagnostics, but cross-reactions prevent accurate species identification. Recently, genetic characterisation has confirmed that A. marginale and A. centrale are separate species. Diversity studies targeting Msp1a repeats for A. marginale and Msp1aS repeats for A. centrale have revealed high genetic variation and point to correspondingly high levels of variation in A. marginale outer membrane proteins (OMPs), which have been shown to be potential vaccine candidates in North American studies. Information on these OMPs is lacking for South African A. marginale strains and should be considered in future recombinant vaccine development studies, ultimately informing the development of regional or global vaccines. View Full-Text
Keywords: bovine anaplasmosis; qPCR; msp1α genotyping; Msp1a; Msp1aS bovine anaplasmosis; qPCR; msp1α genotyping; Msp1a; Msp1aS
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
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Hove, P.; Khumalo, Z.T.H.; Chaisi, M.E.; Oosthuizen, M.C.; Brayton, K.A.; Collins, N.E. Detection and Characterisation of Anaplasma marginale and A. centrale in South Africa. Vet. Sci. 2018, 5, 26.

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