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Vet. Sci. 2018, 5(1), 25; https://doi.org/10.3390/vetsci5010025

Intrauterine Transmission of Anaplasma phagocytophilum in Persistently Infected Lambs

Section for Small Ruminant Research, Department of Production Animal Clinical Sciences, Norwegian University of Life Sciences, N-4325 Sandnes, Norway
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Received: 28 January 2018 / Revised: 23 February 2018 / Accepted: 26 February 2018 / Published: 28 February 2018
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Abstract

Anaplasma phagocytophilum, which causes the disease tick-borne fever (TBF), is the most important tick-borne pathogen in European animals. TBF may contribute to severe welfare challenges and economic losses in the Norwegian sheep industry. The bacterium causes a persistent infection in sheep and several other animal species. The objective of this study was to investigate whether intrauterine transmission occurs in persistently infected sheep. The study included thirteen 5–6-month-old unmated ewes, of which twelve were experimentally infected with A. phagocytophilum (GenBank acc. no. M73220). Four to six weeks later, all ewes were mated, and nine became pregnant. Blood samples were collected from these ewes and their offspring. If the lamb died, tissue samples were collected. The samples were analyzed with real-time PCR (qPCR) targeting the msp2 gene. PCR-positive samples were further analyzed by semi-nested PCR and 16S rDNA sequencing. A total of 20 lambs were born, of which six died within two days. Six newborn lambs (30%) were PCR-positive (qPCR), of which one was verified by 16S rDNA sequencing. The present study indicates that intrauterine transmission of A. phagocytophilum in persistently infected sheep may occur. The importance of these findings for the epidemiology of A. phagocytophilum needs to be further investigated. View Full-Text
Keywords: Anaplasma phagocytophilum; persistence; intrauterine transmission; sheep Anaplasma phagocytophilum; persistence; intrauterine transmission; sheep
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
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Stuen, S.; Okstad, W.; Sagen, A.M. Intrauterine Transmission of Anaplasma phagocytophilum in Persistently Infected Lambs. Vet. Sci. 2018, 5, 25.

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