Intrauterine Transmission of Anaplasma phagocytophilum in Persistently Infected Lambs
AbstractAnaplasma phagocytophilum, which causes the disease tick-borne fever (TBF), is the most important tick-borne pathogen in European animals. TBF may contribute to severe welfare challenges and economic losses in the Norwegian sheep industry. The bacterium causes a persistent infection in sheep and several other animal species. The objective of this study was to investigate whether intrauterine transmission occurs in persistently infected sheep. The study included thirteen 5–6-month-old unmated ewes, of which twelve were experimentally infected with A. phagocytophilum (GenBank acc. no. M73220). Four to six weeks later, all ewes were mated, and nine became pregnant. Blood samples were collected from these ewes and their offspring. If the lamb died, tissue samples were collected. The samples were analyzed with real-time PCR (qPCR) targeting the msp2 gene. PCR-positive samples were further analyzed by semi-nested PCR and 16S rDNA sequencing. A total of 20 lambs were born, of which six died within two days. Six newborn lambs (30%) were PCR-positive (qPCR), of which one was verified by 16S rDNA sequencing. The present study indicates that intrauterine transmission of A. phagocytophilum in persistently infected sheep may occur. The importance of these findings for the epidemiology of A. phagocytophilum needs to be further investigated. View Full-Text
Share & Cite This Article
Stuen, S.; Okstad, W.; Sagen, A.M. Intrauterine Transmission of Anaplasma phagocytophilum in Persistently Infected Lambs. Vet. Sci. 2018, 5, 25.
Stuen S, Okstad W, Sagen AM. Intrauterine Transmission of Anaplasma phagocytophilum in Persistently Infected Lambs. Veterinary Sciences. 2018; 5(1):25.Chicago/Turabian Style
Stuen, Snorre; Okstad, Wenche; Sagen, Anne M. 2018. "Intrauterine Transmission of Anaplasma phagocytophilum in Persistently Infected Lambs." Vet. Sci. 5, no. 1: 25.
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.