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Vet. Sci. 2018, 5(1), 27; https://doi.org/10.3390/vetsci5010027

The Performance of Three Immune Assays to Assess the Serological Status of Cattle Experimentally Exposed to Mycoplasma bovis

1
Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, St Lucia, QLD 4072, Australia
2
School of Veterinary Science, The University of Queensland, Gatton, QLD 4343, Australia
3
Asia-Pacific Centre for Animal Health, Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, VIC 3010, Australia
4
Vaccine and Infectious Disease Organization-International Vaccine Centre (VIDO-InterVac), 120 Veterinary Road, Saskatoon, SK S7N 5E3, Canada
*
Author to whom correspondence should be addressed.
Received: 1 February 2018 / Revised: 2 March 2018 / Accepted: 6 March 2018 / Published: 8 March 2018
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Abstract

Mycoplasma bovis is associated with several clinical syndromes of cattle. Currently, limited information is available on the sensitivity (Se) and specificity (Sp) of serological assays used for the detection of M. bovis-specific antibodies. Consequently, it is difficult to critically evaluate the outcomes of studies that use these assays. Therefore, the current study used bovine sera sourced from M. bovis exposure studies from three countries to estimate the Se and Sp of two commercial M. bovis enzyme-linked immunosorbent assays (ELISA), BIO K302 and BIO K260, and Western blotting. Western blotting had the highest Se estimate of 74% (95% confidence interval (CI): 16–98%), compared to the BIO K302: 47% (95% CI: 10–87%) and BIO K260: 28% (95% CI: 1–92%). However, for Sp, the BIO K302: 96% (95% CI: 87–99%) and the BIO K260: 100% (95% CI: 93–100%) out-performed Western blotting: 88% (95% CI: 56–98%). Western blotting was the best assay for detecting seroconversion, correctly identifying 61% (95% CI: 29–86%) of exposed animals compared to 35% for BIO K302 (95% CI: 21–54%) and 8% for BIO K260 (95% CI: 0–87%). While none of the methods assessed had high Se and Sp, the availability of these estimates will aid in the interpretation of studies that use these assays. The results of this study highlight the difficulties encountered when using serology to detect exposure to M. bovis in cattle. View Full-Text
Keywords: Mycoplasma bovis; serology; sensitivity; specificity; ELISA; Western blotting Mycoplasma bovis; serology; sensitivity; specificity; ELISA; Western blotting
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Schibrowski, M.L.; Barnes, T.S.; Wawegama, N.K.; Vance, M.E.; Markham, P.F.; Mansell, P.D.; Marenda, M.S.; Kanci, A.; Perez-Casal, J.; Browning, G.F.; Gibson, J.S.; Mahony, T.J. The Performance of Three Immune Assays to Assess the Serological Status of Cattle Experimentally Exposed to Mycoplasma bovis. Vet. Sci. 2018, 5, 27.

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