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Vet. Sci. 2018, 5(1), 12; https://doi.org/10.3390/vetsci5010012

Molecular Identification of Eimeria Species in Broiler Chickens in Trinidad, West Indies

1
Department of Basic Veterinary Sciences, School of Veterinary Medicine, The University of the West Indies (St. Augustine), Eric Williams Medical Sciences Complex, Mount Hope, Republic of Trinidad and Tobago
2
Department of Pathobiology and Population Sciences, The Royal Veterinary College, Hertfordshire AL9 7TA, UK
3
School of Veterinary Medicine, The University of the West Indies (St. Augustine), Eric Williams Medical Sciences Complex, Mount Hope, Republic of Trinidad and Tobago
4
Department of Veterinary Clinical Sciences, School of Veterinary Medicine, The University of the West Indies (St. Augustine), Eric Williams Medical Sciences Complex, Mount Hope, Republic of Trinidad and Tobago
*
Author to whom correspondence should be addressed.
Received: 7 December 2017 / Revised: 15 January 2018 / Accepted: 17 January 2018 / Published: 22 January 2018
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Abstract

Coccidiosis is an intestinal disease of chickens of major economic importance to broiler industries worldwide. Species of coccidia found in chickens include Eimeria acervulina, Eimeria brunetti, Eimeria maxima, Eimeria mitis, Eimeria necatrix, Eimeria praecox, and Eimeria tenella. In recent years, polymerase chain reaction (PCR) has been developed to provide accurate and rapid identification of the seven known Eimeria species of chickens. The aim of this study was to use species-specific real-time PCR (qPCR) to identify which of the seven Eimeria species are present in Trinidad poultry. Seventeen pooled fecal samples were collected from 6 broiler farms (2–5 pens per farm) across Trinidad. Feces were also collected from birds showing clinical signs of coccidiosis in two live bird markets (pluck shops). qPCR revealed the presence of five species of Eimeria (E. acervulina, E. maxima, E. mitis, E. necatrix, and E. tenella), but not E. brunetti or E. praecox. Mixed infections were detected on all broiler farms, and DNA of two highly pathogenic Eimeria species (E. tenella and E. necatrix) was detected in feces taken from clinically sick birds sampled from the two pluck shops. View Full-Text
Keywords: coccidiosis; coccidia; Eimeria; poultry; Trinidad coccidiosis; coccidia; Eimeria; poultry; Trinidad
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Brown Jordan, A.; Blake, D.; Beard, J.; Beharry, A.; Serrette, L.; Soleyn, A.; Sookhoo, J.; Blake, L.; Brown, G.; Oura, C. Molecular Identification of Eimeria Species in Broiler Chickens in Trinidad, West Indies. Vet. Sci. 2018, 5, 12.

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