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Beverages 2018, 4(2), 40; https://doi.org/10.3390/beverages4020040

Prevalence and Characteristics of Listeria monocytogenes Isolates in Raw Milk, Heated Milk and Nunu, a Spontaneously Fermented Milk Beverage, in Ghana

1
Department of Applied Biology, Faculty of Applied Sciences, University for Development Studies, P.O. Box 24, Navrongo Campus, Ghana
2
Department of Biotechnology, Faculty of Agriculture, University for Development Studies, P. O. Box TL 1882, Nyankpala, Ghana
3
Department of Food Science, University of Copenhagen, Rolighedsvej 26, DK 1958 Frederiksberg C, 1958 Copenhagen, Denmark
*
Author to whom correspondence should be addressed.
Received: 20 April 2018 / Revised: 12 May 2018 / Accepted: 21 May 2018 / Published: 23 May 2018
(This article belongs to the Special Issue Microbiological Safety of Beverages)
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Abstract

Listeria monocytogenes is a gram-positive food-borne pathogen that causes listeriosis in humans. Currently, there is little information on the prevalence of Listeria monocytogenes in raw milk and traditional yoghurt-like milk beverage, nunu, in Ghana. The purpose of this study was to investigate the prevalence of L. monocytogenes isolates in raw cow milk, boiled milk and nunu in Ghana, and to characterize these L. monocytogenes isolates according to their serogroups, virulence potentials and antibiotic susceptibility profiles. A total of 254 samples comprising 114 raw cow milk, 56 boiled milk and 84 nunu were collected from dairy farms and market vendors for detection of L. monocytogenes. The overall prevalence of L. monocytogenes in raw milk, boiled milk and nunu was 5.5% (14/254). Listeria monocytogenes was prevalent in raw cow milk (8.8%; 10/114) and nunu (13.1%; 11/84), while no Listeria spp. was not detected in boiled milk. A total of 62 L. monocytogenes isolates were analysed to belong to molecular serogroups 1/2a-3a (32/62, 51.6%), 1/2b-3b-7 (14/62, 22.6%), 4b-4d-4e (9/62, 14.5%) and 1/2c-3c (7/62, 11.3%). All 62 L. monocytogenes isolates harbored the virulence-associated genes inlA, inlB, inlC, inlJ, plcA, actA, hlyA, iap and prfA. All Listeria monocytogenes in the present study were generally susceptible to the tested antibiotics, except neomycin and tetracycline, for which phenotypic resistance was observed among isolates. View Full-Text
Keywords: dairy products; food safety; virulence genes; antimicrobial resistance; listeria dairy products; food safety; virulence genes; antimicrobial resistance; listeria
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
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Owusu-Kwarteng, J.; Wuni, A.; Akabanda, F.; Jespersen, L. Prevalence and Characteristics of Listeria monocytogenes Isolates in Raw Milk, Heated Milk and Nunu, a Spontaneously Fermented Milk Beverage, in Ghana. Beverages 2018, 4, 40.

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