Next Article in Journal
Nutraceutic Characteristics of the Extracts and Juice of Chayote (Sechium edule (Jacq.) Sw.) Fruits
Previous Article in Journal
Phenolic Compounds in Fruit Beverages
Article Menu

Export Article

Beverages 2018, 4(2), 36; doi:10.3390/beverages4020036

Review
Microbial and Chemical Diversity of Traditional Non-Cereal Based Alcoholic Beverages of Sub-Saharan Africa
Department of Biological Sciences and Biotechnology, Botswana International University of Science and Technology, Private Bag 16, Central District, Palapye, Botswana
*
Author to whom correspondence should be addressed.
Received: 18 December 2017 / Accepted: 17 March 2018 / Published: 1 May 2018

Abstract

:
Fermentation remains an important food preparation technique of health, cultural and economic importance throughout the world. In Sub-Saharan Africa, traditional alcoholic fermentation of cereal and non-cereal based substrates into alcoholic beverages is deeply rooted in the society. Although a multitude of traditional alcoholic beverages from cereal substrates are well researched and documented, their non-cereal based counterparts, mostly produced from indigenous, inexpensive substrates, remain less well studied. In addition, reports of health problems associated with non-cereal based alcoholic beverages produced from spontaneous fermentation are a major cause of concern. This review aims to highlight the microbiological and chemical profiles of these non-cereal based alcoholic beverages with a focus on the Sub-Saharan region. Here, we underscore the importance of the microbial repertoire and the substrates thereof in attaining aromatic complexity and a characteristic taste in these beverages. These aspects are an important starting point towards the potential commercialization of these complex aromatic non-cereal based traditional beverages.
Keywords:
non-cereal; fruit; beverage; fermentation; microbes; metabolites

1. Introduction

Fermentation is the oldest and most popular technology used by humankind, dating back to the Neolithic period. Its exploitation was mostly used in dairy products, baking, wine making, and brewing. With the advent of new technologies, modern exploitation of fermentation now extends to the production of renewable fuels and biopharmaceuticals. From the traditional viewpoint, food fermentation is vital, as it improves the nutritional composition of foods, their sensory properties, and their acceptability, as well as it prolonging the shelf life of food [1]. In addition, fermentation reduces toxic or anti-nutritional food components such as phytic acids, polyphenolic compounds, and tannins [2] in food and beverages from plant material. To date, different parts of the world boast of a multitude of different alcoholic beverages. Due to cultural differences, these beverages are either cereal or non-cereal based. For example, in the Sub-Saharan region, non-cereal based alcoholic beverages are produced from inexpensive substrates such as wild as well as cultivated fruits, tree saps, roots, and tubers whereas cereal-based alcoholic beverages, are made from small grains; maize, sorghum, and millet [3,4].
Several cereal-based alcoholic beverages in the Sub-Saharan region have been well documented [5,6,7,8]. The fermentative microbes and chemical profiles of the resulting alcoholic beverages are well known. These include; chikokivana, a spirit produced from a mixture of maize meal, and millet malt popular in Zimbabwe [9], togwa of Tanzania, produced from sorghum, maize, and millet [10,11], burukutu, and pito—both popular indigenous alcoholic beverages of Nigeria produced from sorghum (Sorghum vulgare and Sorghum bicolor) [6] as well as borde of Ethiopia, produced from maize, barley or wheat and their malts [12]. One of these cereal-based alcoholic beverages—chibuku—another sorghum-based beer, has made it to the commercial markets in Botswana, Zimbabwe and South Africa [5]. A consortium of yeasts and bacteria is known to carry out fermentation and contribute to unique sensorial properties of these beverages. Fermentation in the production of burukutu is carried by Saccharomyces cerevisiae, Saccharomyces chavelieri, Leuconostoc mesenteroides, Candida spp. and Acetobacter spp. [13] whilst traditional opaque beers (such as doro and chikokivana) of Zimbabwe are fermented by Saccharomyces cerevisiae, Issatchenkia occidentalis, Kluyveromyces marxianus, Candida glabrata, Sporobolomyces holsaticus and Rhodotorula spp. [14].
Currently in the Sub-Saharan Africa region, there is less documentation of microorganisms involved in fermentation of non-cereal-based alcoholic beverages as compared to cereal-based alcoholic beverages. The deficiencies in research of the rich biodiversity of microorganisms and sometimes unique substrates that are present in sub-Saharan Africa pose a challenge for commercialization and even improvement of the standard of product produced at household level. There is an increasing trajectory of research output in different countries on their non-cereal-based alcoholic beverages. The research includes the microbial and chemical diversity of their traditional beverages which are produced from different substrates. Examples of the traditional alcoholic beverages include cachaça (produced from fresh sugar cane juice), taruba (produced from cassava) and caxiri (produced from cassava and sweet potatoes) of Brazil [15,16,17,18,19], kefir (produced from goat, cow and sheep milk) of Russia [20,21], koumiss (produced from milk) of Russia and Mongolia [20,22,23], pulque (produced from Agave plants) of Mexico [15,16,17], toddy (produced from palm tree sap) of India [24], and airag (produced from mare or camel milk) of Mongolia [25] to list a few.
Non-cereal-based alcoholic beverages can pose health problems like food poisoning and food intoxication [26]. Although similar issues have also been observed with regards to cereal-based alcoholic beverages, there have been more media reports of direct deaths from non-standard fermentation of non-cereal-based alcoholic beverages. Knowing the fermentative microorganisms and resultant chemical profiles of these non-cereal-based alcoholic beverages could benefit the development of starter cultures for both household and commercial purposes, thus improving the final product quality and safety as well as poising these beverages well for potential commercialization. This review aims to highlight the microbiological and chemical profiles of non-cereal-based alcoholic beverages with a focus on the Sub-Saharan region.

2. Non-Cereal-Based Alcoholic Beverages

Non-cereal-based beverages are less commonly consumed in comparison to the cereal-based beverages in the Sub-Saharan region. The abundance of a variety of cereals across the continent could explain the discrepancy. These non-cereal based beverages can be either non-alcoholic or alcoholic. Although non-alcoholic beverages are important based on the nutritional benefits that they offer, the sensory properties of alcoholic beverages make them more popular. In sub-Saharan Africa, fermented alcoholic beverages are produced from various inexpensive local raw materials such as palm sap, marula fruit and mogwana. Figure 1, Figure 2 and Figure 3 show examples of cheap substrates normally used. Some brews have even been commercialized such as A. marula liqueur from the marula fruit (Figure 2) [5] and tej (Figure 4) [27,28]. Tej is a home processed, honey wine of Ethiopia produced from a mixture of honey and sugar as major fermentable substrates. Traditional alcoholic beverages are usually preferred over their western counterparts as they are inexpensive to produce at household levels and affordable for consumers, especially for low-income earners.
The mass production of non-cereal-based alcoholic beverages of Sub-Saharan Africa relies on uncontrolled spontaneous fermentation and exploits inexpensive substrates that are available in the locality, viz. palm tree fruits (Arecaceae family) and exudates, sugarcane (Saccharum officinarum), banana pulp (Musa acuminata), watermelons (Citrullus lanatus), Hyphaene petersiana (mokolwane/moxao) (Figure 1), marula fruit (Sclerocaryabirrea) (Figure 2), Grewia flava (mogwana) (Figure 3), Grewia occidentalis (moretlwa), Grewia flavascens (mokgomphatha), Popowiaobovata, Balanites aegyptiaca, Berchemia discolor, Ziziphus mauritiana (masau), Kedrostis hirtella (mogakangwaga), Khadia acutipetala, cassava (Manihot esculenta), and honey [5,9,29,30]. Some of the beverages made from non-cereal substrate include muchema, palm wine, setopoti, bojalwa-ja-morula, khadi, mukumbi, urwagwa, pineapple wine, and tej. The characteristics of the substrates are summarized in Table 1. The fermentation of non-cereal-based beverages, although not all fully characterized, is reported to be carried out by yeasts, lactic acid and acetic acid bacteria during a spontaneous fermentation production procedure. Such a technique associated with mixed culture fermentation, as shown in Table 2, may influence the distinctive flavor complexity of these traditional non-cereal-based alcoholic beverages. Table 2 summarizes the microbial diversity of non-cereal-based alcoholic beverages of sub-Saharan Africa region as well as their secondary metabolites.

3. Commercialization of Non-Cereal-Based Alcoholic Beverages

Non-cereal-based alcoholic beverages have a great potential for commercialization that is comparable to common European and Mediterranean alcoholic beverages. As such, these beverages need to be duly studied and documented, and their production processes standardized and modernized towards optimization. Local producers are the bearers of the indigenous knowledge associated with processing of the non-cereal-based alcoholic beverages. The inclusion of the locals that produce these beverages in the research and commercialization processes will result in benefits such as employment and contribution towards the countries’ economic development. One of the challenges that exists with these beverages however, is that they age or mature very rapidly thus losing flavor, microbial stability, as well as their palatability, hence making their commercialization challenging [38].
It is without doubt that nutritional value and safety could be improved by research and commercialization of traditional alcoholic beverages. The natural microflora of the substrates can also serve as a source of novel non-conventional yeast strains with extra supplementary health benefits and aromatic complexity features. Extensive sensory properties of fermentation products from non-conventional yeast strains have been proposed to enhance beer flavor [61].

4. Advancements in Fermentation Strategies

Traditional fermentation processes are customarily uncontrolled and depend on the microorganisms from the environment or the normal microflora of the substrate to initiate fermentation. Such fermentation processes often result in variable yield and quality of the beverage [62]. The most common traditional beverages are products of spontaneous or batch accelerated fermentation. Fermentation in the former is mostly due to the presence of resident yeast flora of the fruit surfaces, or from brewing equipment, whereas the latter involves pitching of the mixture to be fermented with a previously fermented brew. The production of traditional non-cereal-based alcoholic beverages usually involves use of traditional fermentation vessel which are typically clay pots (Figure 5) covered with a cloth, producing wines and spirits, if distilled. The cloths create a slightly anaerobic environment but in some instances, the fermentation vessel is completely closed to create stricter anaerobic conditions. Repurposed polyethylene drums are also increasingly used as fermentation vessels in traditional settings.
Natural or spontaneous fermentation does not consistently produce products with desirable flavors. The natural microbial flora in the raw material (substrate) may not always be the same just as the substrate will vary depending on different environmental conditions. The secondary metabolites formed during oxidation of the fruit acids and alcohols post-fermentation lead to difficulty in production of a product with consistent characteristics [63,64]. This variance in taste and aroma is inherent and akin to differences of wine bouquets. Other factors that might influence reproducibility include temperature, contamination, type of fermentation vessel, and fermentation time.
A drawback of natural or spontaneous fermentation is that there is a possibility of product failure due to a reduced fermentation progression rate or an uncharacteristic product formation due togrowth of undesirable flora and disease-causing microbes [32,33]. When back-slopping is used, the previous fermentis usually dominated by fermentative microorganisms, which solves to some extent the problem of variability of the product [5,9]. This is the most common practice at household level, in the production of many traditional alcoholic beverages. One disadvantage of this technique however, may be that toxic compounds that are normally less toxic in smaller amounts keep concentrating to toxic levels in the subsequent brews.
Batch-to-batch variability of alcoholic fermentation can be minimized by introduction of a pure commercial starter culture. Due to increasing customer preference for a variety of products, a consortium of different microbes can also be used. Common consortia harbor yeasts, acetic acid, and lactic acid bacteria [34]. The diversity in carbon metabolism pathways among the different microbes in the consortia, allows mixed culture fermentation towards the production of different primary and secondary metabolites that influence the overall aromatic complexity of the alcoholic beverage [65,66,67]. Such mixed culture fermentation has become common in the wine and beer industries in an effort to improve the distinctive flavors of the alcoholic beverages. For example, the use of S. cerevisiae and T. delbrueckii lead to the production of beverages with elevated levels of higher alcohols, ethyl acetate and isoamyl acetate, and lower levels of ethyl hexanoate and ethyl octanoate [61] thus enhancing the taste and flavor. In the wine industry starter cultures are dominated by Saccharomyces species, but the need for new tastes to sort customer demands have presented great potential for non-Saccharomyces species to be used as part of the microbial consortia for fermentation [63,68]. To improve product quality and facilitate the production of a consistent product, the commercialization of non-cereal-based alcoholic beverages would therefore require cultures made up of microbial consortia.

5. Microbial Consortia for Fermentation

5.1. Yeasts

Yeasts are the most predominant fermentative microorganisms responsible for spontaneous fermentation of non-cereal-based alcoholic beverages. This is probably because yeasts are naturally found on the tree barks, leaves, and skins of fruits [69]. Grapes for example, harbor a number of fermenting yeasts on their skins such as Kloeckera spp., Hanseniaspora spp., Candida spp., Pichia spp., Kluyveromyce spp., Metschnikowia spp., and Cryptococcus spp. [67,70,71] with Hanseniaspora uvarum, Pichia terricola, and Metschnikowia pulcherrima being the three most representative species isolated to date [69]. The most well-known and best fermenting yeast, S. cerevisiae, however, is found in very low frequencies on some fruits such as the grape skins, and in vineyards soils [72]. Documented evidence suggest that in the early stages of fermentation, fruit surfaces are dominated by non-Saccharomyces yeasts, bacteria, and filamentous fungi [73,74] but Saccharomyces yeasts dominate the microbial population at the end of fermentation in anaerobic conditions. This suggests that Saccharomyces yeasts are the most dominant alcoholic fermentation microorganisms [75,76,77]. Even in the presence of oxygen, S. cerevisiae, is the predominant yeast species responsible for the production of ethanol [78,79]. The production of ethanol, toxic to other microbes, has been hypothesized to be a niche defense mechanism for S. cerevisiae [42].
Alcoholic fermentation is not exclusive to S. cerevisiae [19,26,47]. An important attribute of a brewer’s yeast—an ability to ferment simples sugars, even in the absence or presence of excess O2 [80], is present in non-conventional yeasts. Examples of non-conventional yeasts that have been isolated from alcoholic beverages include Saccharomycodes ludwigii [81], Candida tropicalis [82], Torulaspora delbrueckii, Zygosaccharomyces rouxii [83], and Pichia kluyveri [22].
Brettanomyces spp., Candida spp., Cryptococcus spp., Debaryomyces spp., Dekkera spp., Galactomyces spp., Geotrichum spp., Hansenula spp., Hanseniaspora spp., Hyphopichia spp., Issatchenkia spp., Kazachstania spp., Kluyveromyces spp., Metschnikowia spp., Pichia spp., Trichosporon spp., Rhodotorula spp., Rhodosporidium spp., Saccharomyces spp., Saccharomycopsis spp., Schizosaccharomyces spp., Saccharomycodes spp., Sporobolomyces spp., Torulopsis spp., Torulaspora spp., Zygosaccharomyces spp., and Yarrowia spp. are among several species of non-conventional yeast strains isolated from fermented foods and alcoholic beverages elsewhere in the world [11]. A list of yeasts species responsible for fermentation of alcoholic beverages in the Sub-Saharan region is shown in Table 2.

5.2. Bacteria

Bacteria are known to ferment simple sugars but the end product is usually non-alcoholic if allowed to ferment exclusively. Exceptions of alcohol producing bacteria such as Zymomonas mobilis, Clostridium acetobutylicum, and Klebsiella pneumonia exist. Z. mobilis is a natural ethanologen which possess desirable characteristics such as high specific productivity, high alcohol tolerance, a broad pH range for production (pH 3.5 to 7.5) and it is generally regarded as safe (GRAS) [84]. The bacterium is mostly utilized for biofuel production [85] although it has also been isolated from some alcoholic beverages such as pulque [11,54] and palm wine [86] where it is responsible for the production of ethanol, CO2, acetaldehyde and H2S [87]. C. acetobutylicum ferments sugars into a mixture of organic solvents namely acetone, butanol, and ethanol [88,89] while K. pneumonia produces ethanol, lactate, and acetate [90]. However, their applications in alcoholic beverages are not ideal for human consumption because they produce high levels of acetone and butanol which are harmful to humans.
Lactic acid bacteria (LAB) are known for the sour taste and low pH they confer to the product due to lactic acid production. LAB are present in a significant number of fermented alcoholic beverages (Table 2) probably because of their ubiquity in nature. In the brewing of alcoholic beverages, these bacteria bring about multistep transformation of the raw material (carbohydrate substrates), which would be otherwise impossible to accomplish with yeasts alone. For example, when a substrate cannot be utilized by yeasts alone, the LAB can break it down into simple monomers thus allowing better utilization of the substrate. Major genera of the LAB species isolated from various fermented beverages include Alkalibacterium spp., Carnobacterium spp., Enterococcus spp., Lactobacillus spp., Lactococcus spp., Leuconostoc spp., Oenococcus spp., Pediococcus spp., Streptococcus spp., Tetragenococcus spp., Vagococcus spp., and Weissella spp. [91].
Acetic acid bacteria are also found in most traditional alcoholic beverages contributing to the pH and taste of beverages through the production of acetic acid [92]. The combination of lactic acid and acetic acid have been reported to enhance the flavor of alcoholic beverages [9,60,93,94]. Acetic acid is produced from the conversion of ethanol by Acetobacter spp. under aerobic conditions making the alcoholic beverage sour due to an increase in titratable acidity as well as a lower pH of the beverage [10,12,62].

6. Microbial Identification Methods

The isolation and correct identification of microbial isolates from a complex microbial community associated with the substrate used for fermentation as well the product of fermentation is a very important starting point for commercialization of non-alcoholic beverages. Recovery of microbes from substrates involves classical extensive sampling, incubation in media of choice depending on the purpose of the study, and then subsequent selection of colonies for further characterization. There are a number of reviews giving comprehensive details on how microbes involved in fermentation are isolated [11,95,96,97]. Herein we outline some of the identification methods available and their advancements.
Classical identification methods (such as culturing, enumeration, isolation, substrate utilization test, substrate assimilation test, staining and biochemical tests, to list a few) entailing the characterization of morphological, biochemical and physiological traits, have been widely accepted and described in the following texts [9,34,98,99]. The classical identification methods have several limitations including incorrect identification, poor resolution and inconsistent reproducibility due to the dependence on the physiological state of the cells when compared to molecular identification methods. Molecular identification methods can be classified into culture dependent and culture independent techniques.

7. Culture Dependent Identification of Microbes

The use of culture media to isolate microbes from substrates used for fermentation as well as microbes found in alcoholic beverages is the most prominent method. The method involves aseptic acquisition of material and growing microbes in culture and choice before identification of the colonies of choice using DNA. The principles that underlie molecular methods have been extensively addressed [99]. In brief, the amplification, restriction analysis as well as sequencing of ribosomal DNA has been used to quantify and characterize the microbial consortia in alcoholic beverages. The field is evolving fast as extensively reviewed elsewhere [100]. Non-cereal-based alcoholic beverages such as tej, burukutu, agadagidi and mukumbi have had their microbial characterization performed using culture dependent molecular methods [13,27,101,102].
The culture-dependent methods have added value by giving insight on the sugars the microorganisms can utilize during fermentation. For example, some of the yeasts isolated from masau fruits are able to utilize glucose, sucrose, maltose, galactose and raffinose while others were able to assimilate glucose, galactose, cellobiose, lactose, maltose, saccharose, trehalose, melezitose, raffinose, L-lysine, cadaverine and creatine [9]. The assimilation test is essential as it signifies which is the best chemical media to use for the isolated microbe at industrial scale, ensuring maximum utilization of the substrate to produce the product. The pitfall of culture-dependent methods however, it that they underestimate the species richness of non-culturable microorganisms that may play major roles in production of fermented beverages. Examples of culture-dependent identification methods include DNA extraction from cultured microbes and the use of species-specific PCR primers for species level identification [11].

8. Culture-Independent Identification of Microbes

While microbial consortia involved in fermentation include bacteria, only 1% of bacteria are cultivable. Profiling of both culturable and non-culturable microbial populations from fermented beverages is therefore attractive. Culture-independent methods entail the use of molecular methods without first culturing microbes on media. Culture-independent methods such as direct DNA extraction from fermented products that represent the ecological niche, have therefore become common [35,85,103,104]. The method allows for detection of more species that would otherwise not be detected by culture-dependent methods. Therefore a less biased picture of species richness is possible [103,105]. For best results, both culture-dependent and culture-independent methods are used simultaneously. For instance in the work performed by Stringini et al. on palm wine, culture-dependent methods detected S. cerevisiae (as the predominant species), S. ludwigii and Z. bailii while a culture-independent method employing denaturing gradient gel electrophoresis (DGGE) analysis further detected H. uvarum, C. parapsilopsis, C. fermentati and P. fermentans [81] from the same sample.

9. Chemical Products of Fermentation

During alcoholic fermentation, a multitude of aromatic primary and secondary metabolites are produced [105]. Primary metabolites are products of metabolism that are produced during the growth phase of an organism. These perform the physiological functions and support in the overall development of the cell such as hormones, glycerol and enzymes [106,107,108]. Secondary metabolites, on the other hand, are the end products of primary metabolism that are synthesized after the growth phase has been completed. Such metabolites are vital in the ecological function of the cell. Examples of secondary metabolites include esters, alkanols, organic acids, phenols, aldehydes and ketones (Figure 6). The combination, variety and abundance of aromatic molecules are pertinent to sensory properties of non-cereal-based alcoholic beverages and thus their unique flavor profile.
Different substrates have different types and amounts of fermentable sugars and amino acids. The presence of different microorganisms in different concentrations during fermentation, each with their varying fermentable sugar preference and primary metabolic requirements leads to various species-specific pathways being turned on and resulting in production of different secondary metabolites. This is shown in both Table 1 and Table 3. These attributes affect the overall taste and flavor of alcoholic beverages. Differential production of higher alcohols amongst microorganisms contributes to the distinctive beer flavors. Examples of higher alcohols that intensify flavor characteristics of beers include n-propanol, iso-butanol, 2-methylbutanol and 3-methlybutanol [109]. Due to their known antifungal activity, higher alcohols can also increase shelf life of the alcoholic beverage by acting as a preservative. Some of the secondary metabolites such as 2-methyl-1-propanol, 2/3-methyl-1-butanol, ethyl lactate and ethyl acetate, which are typical aromatic volatiles reported in beer, wines and spirits, have been isolated in non-cereal-based alcoholic beverages such as khadi [56]. Figure 6 summarizes secondary metabolites produced by yeasts.
Esters are formed through esterification of activated fatty acids and alcohols [109]. The amount of esters produced during fermentation depends on the abundance of the corresponding alcohols and acyl CoAs [67]. Esters can either be found in the starting material albeit at lower concentrations [110] or formed intracellular by microorganisms. This is the most important group of secondary metabolites essential for aromatic characteristics as they impart a fruity and sweet note to alcoholic beverages during fermentation. Esters are divided into acetate esters (synthesized from a higher alcohol or ethanol with acetic acid) and medium-chain fatty acid ethyl esters (made from a medium-chain fatty acid and an ethanol radical) [111,112].
Some secondary metabolites act as preservatives, some of which include organic acids such as oxalic, citric, tartaric, malic, ascorbic, fumaric, lactic, and acetic acids. Organic acids are responsible for the rapid acidification of alcoholic beverages [113]. They lower pH to below 4.6, which inhibits the growth of unwanted spoilage microorganisms [10].
Not all secondary metabolites contribute positive traits to the alcoholic beverages. For example, acetaldehyde has an unpleasant pungent odor at higher concentrations, imparting a freshly cut green leaves flavor to the beverage [114]. Methanol and its metabolites such as formaldehyde and formic acid, are produced during microbial fermentation of alcoholic beverages like kachasu of Zimbabwe [37] and pose health challenges due to their toxicity effects [115]. The toxicity of kachasu due to the presence of isoamyl alcohol, iso-butanol, acetone, methanol, ethylacetate, and furfurals has led to the prohibition of kachasu in Zimbabwe since 1971 [5]. Methanol toxicity in distillates has also been reported in kanyanga (although a cereal-based distillate) of Burundi [116]. Secondary metabolites of alcoholic fermentation are summarized in Table 3. Their contribution to the flavor of the alcoholic beverage is also briefly noted.

10. Influence of Ecology on Fermentation Microbes

The substrates used in fermentation provide ecological niches for different microbial species [70]. An ecological niche is an ecosystem that comprises of microbial species within a particular environment. The precise ecological niche of a microbeis primarily determined by the specific metabolic properties of that organism. The microbes are characterized by different physiological traits, which account for a plethora of effects upon production of the beverages. Microbial consortia found in beverages after fermentation are only those that manage to survive and reproduce in the beverage.
The abundance of the microbes before and after fermentation is generally not proportional. Due to limited literature, the ecological niches are not well understood but microbial diversity on the substrates suggests that the substrates have enough sugars that help the microbes survive and influence fitness for competitive survival of particular species. Additionally, the pH levels of these substrates (Table 1) and the resultant chemical ecology produced by the fermentation process would also have a great influence on the microflora that would dominate fermentation.
Some of the studied ecological niches associated with non-cereal based beverages include the masau fruits (Ziziphus mauritiana) [9], unfermented palm sap [123], marula fruits [124] and honey [125]. The masau fruits (Ziziphus mauritiana), used to produce kachasu, have had their ecology studied using unripe, ripe, dried and fermented fruits. The fruits harbored different microbes which included fermentative and non-fermentative yeasts. A. pullulans, Cryptococcus flavus and R. mucilaginosa were isolated from the unripe fruits, S. cerevisiae, I. orientalis, A. pullulans, Cryptococcus magnus, Z. hellenicus, C. parapsilosis, and C. pyralidae were isolated from the ripe fruits, P. fabianii, S. fibuligera, A. pullulans and P. ciferrii were isolated from the dried fruits and, S. cerevisiae, I. orientalis, P. fabianii, S. fibuligera, and H. opuntiae were isolated from the fermented fruits [9]. The above variations show the different fermentative and non-fermentative strains (some of which included non-conventional yeasts) isolated from different ecological niches.

11. Microbial and Chemical Characteristics of Selected Alcoholic Beverages

The chemical complexity of the non-cereal-based alcoholic beverages is due to mixed culture fermentation as illustrated in Table 2. The beverages are produced from varying raw materials which impart unique flavors to the brews. Details of the microbial and chemical characteristics of selected non-cereal based alcoholic beverages are given below.

12. Palm Wine

Palm wine is an alcoholic beverage produced from the spontaneously fermented sap of tropical plants of the Palmae family [123]. The African oil palm (Elaeis guineensis), date palm (Phoenix dactylifera), nipa palm (Nypa fruticans), kithul palm (Caryota urens), and raffia palm (Raphia hookeri) are among the examples of palm trees from which the carbohydrate-rich sap can be obtained. Palm wine has an alcohol content ranging from 1.5% to 7.1% (v/v) and a pH from 4.0 to 5.5 [113]. Palm wine is known the numerous following names in West Africa; mu, bandji, ogogoro, nsafufuo, nsamba, mnazi, and yongo. The beverage is not unique to Africa, as it also popular in India where it is known as toddy or tari [11]. Palm tree sap is tapped (either through inflorescence tapping or stem tapping) and allowed to undergo spontaneous fermentation [35]. Like any other alcoholic beverages, the conversion of the sugary substrate leads to increased nutritional components. The aromatic complexity of the wine is due to the production of compounds produced such as esters, carbonyls, alcohols, phenols, acids, sulphur compounds, terpenes, hydrocarbons, nitrogen compounds, and lactone [63,79,80,126].
Nwachukwu et al. [54] reportedly isolated Staphylococcus spp., Lactobacillus spp., Micrococcus spp., Serratia spp., Bacillus spp., Streptococcus spp., Saccharomyces cerevisiae, and Candida tropicalis from Nigerian palm wine. Similar work on the microbial profile of palm wine-isolated S. cerevisiae, S. chevalieri, Zymomonas mobilis, Hanseniaspora guilliermondii, H. uvarum, Lactobacillus plantarum, Leuconostoc mesenteroides, L. nagelii, L. sucicola, Acetobacter pasteurianus, A. indonesiensis, and Gluconobacter spp. which were detected using both culture-dependent and culture-independent methods [35]. Some of these isolated microbes have also been detected from the unfermented palm sap, meaning they are responsible for the fermentation of the palm wine [81]. Collectively, these investigations suggest that palm wine has diverse fermenting microbes that cooperatively influence the flavors and aromas of the final product.

12.1. Urwagwa

Urwagwa is an alcoholic beverage produced from the fermentation of bananas (Musa acuminata). The banana beverage is common in Rwanda (urwagwa) [42], DR Congo (kasiksi), Burundi (isongo) [116], Uganda (tonto) [127], Tanzania (mbege) [128] and Kenya (urwaga) [127] where it is known by other local names. There have been reports of urwagwa having an alcoholic content ranging from 7% to 18.1% (v/v) [34,84]. The process of fermentation [42] involves the extraction of the banana juice from ripe bananas (which contain glucose, sucrose and fructose) by addition of water to the banana juice in the ratio of 3:1. Filtration of the diluted smooth mixture is carried out through grass held in a clay pot or wooden tank. Germinated, dried and ground sorghum is added to the diluted banana juice and the fermentation broth is then covered with banana leaves and split banana stems and incubated for 2 to 4 days. Saccharomyces cerevisiae is reported as the major fermenting microorganism [116] as well as lactic acid bacteria, yeast, molds and aerobic mesophilic bacteria, but these have not been identified at species level [42].
Spontaneous fermentation rarely is a result of a single fermenting microorganism. The other sources of fermenting microbes in this beverage are likely the clay pot, the grass sieve, the banana leaves, as well as the non-sterile preparation of the fermenting carbohydrate sap. All these sources of spontaneously fermenting microorganisms are never a pure culture. Therefore, further microbial determination needs to be done to identify the fermentative microorganisms. The aromatic profile of the beverage has been linked to secondary metabolites such as esters (ethyl isocyanoacetate, ammonium acetate, ethyl ester and ethyl acetate), alcohols (methyl alcohol, ethanol, 1-propanol, propane-1,3-diol and cyclohexanol) and acids (acetic acid, propanoic acid, methyphosphonic acid, benzoic acid and 5-hexanoic acid) [53].

12.2. Tej

Tej is a home processed, but also commercially available wine produced from honey. The honey is supplemented with sugar before fermentation. A very high alcoholic content of the product has been reported to be in the range of 8.94% to 13.16% (v/v) and pH ranges from 3.56 to 4.45 are common [28]. The honey wine is also produced and consumed in Cameroon where it is known as kuri [129]. Tree barks or roots of some plants or herbal ingredients are added to improve the flavor whereas the fermentation pot is seasoned by smoking over smoldering Rhamnus prinoides stems and olive wood [17,63]. Fermentation is run for 5 days, in warm weather, or for 15 to 20 days, in colder cases [27].
The mixture is stirred daily and finally filtered through a cloth to remove sediment and R. prinoides residues [7]. A yellow, effervescent and cloudy brew is a characteristic of good quality tej (Figure 4). Commercial honey wine (mead) is made using pure cultures of yeasts and they produces aromatic volatile compounds that include alcohols, organic acids, esters, volatile fatty acids, carbonyl compounds, and volatile phenols [111,130]. Aromatic volatile compounds are responsible for the unique flavor of mead as well as its overall sensory characteristics [75,86].
In contrast to the commercial honey wine, S. cerevisiae, Kluyveromyces bulgaricus, Debaromyces phaffi, K. veronae, Zygosaccharomyces rouxii, Hanensula subpeliculosa, S. norbensis, K. vanudenii, Endomycopsis burtonii, Lactobacillus spp., Streptococcus spp., Leuconostoc spp., and Pediococcus spp. have been isolated from the homemade beverage using culture-dependent methods [11,17]. Honey naturally contains various osmotolerant yeasts that prefer low pH environments [120]. Some of which include Saccharomyces spp. (widely found), Rhodotorula spp., Debaryomyces spp., Hansenula spp., Lipomyces spp., Oosporidium spp., Pichia spp., Torulopsis spp., Trichosporon spp., Nematospora spp., Schizosaccharomyces spp., Schwanniomyces spp., Torula spp., and Zygosaccharomyces spp. [125]. These yeasts have been observed to possess the ability to convert glucose and fructose from honey into ethanol and acids. Other species in the genera Saccharomyces spp., Debaryomyces spp., Hansenula spp., Lipomyces spp., Pichia spp., Schizosaccharomyces spp., Torula spp., and Zygosaccharomyces spp. have been reported to have been isolated from honey wine [120].

12.3. Pineapple Wine

Pineapples (Ananascomosus), are cultivated in Angola and they have a significant proportion of sugar and acids that make them ideal for wine production [38]. The production of wine involves fermentation of peeled, sliced and pressed pineapple. The juice is allowed to ferment for 3 days yielding a product with a final alcoholic content of 6.0% to 7.0% (v/v) [38,60] and a pH of approximately 3.78 [59]. Hanseniaspora guilliermondii, Pichiaanomala, Meyerozyma guilliermondii, H. uvarum, Wickerhamomyces anomalus, and H. opuntiae have been reported to be responsible for fermentation [38]. Pineapple wine aroma complexity has been reported to be from compounds such as acetic acid, ethyl acetate, iso-butanol, 3-methyl-1-butanol, 2-methyl-1-butanol, ethyl 2-methylpropanoate, 3-methyl-1-butyl acetate, ethyl hexanoate, methyl benzoate, 2-phenylethanol, methyl 2-methylheptanoate, ethyl benzoate, diethyl succinate, ethyl octanoate, ethyl phenylacetate, 2-phenylethyl acetate, ethyl 9-decenoate, and ethyl decanoate [87,88]. These compounds influence the overall flavor and taste of the wine.

12.4. Muchema

Muchema is an alcoholic beverage produced from the spontaneous fermentation of the Hyphaene petersiana (mokolwane/moxao) tree sap, popular in Shorobe, Botswana. Palms grow well in areas with a shallow, slightly saline water table such as salt pans [131]. Hyphaene petersiana is therefore a common tree species in such areas of Botswana, Namibia, Zimbabwe, Zambia, Tanzania, Angola, Rwanda, Burundi and many other Sub-Saharan countries. The sap that is used to make the beverage is harvested and allowed to ferment for 3to 5 days. The drink is known as omalunga in Namibia but its production is forbidden [57]. There is no literature available with regards to the microbial and chemical profile of this beverage, and thus more research needs to be undertaken.

12.5. Khadi

Khadi is a reddish-brown traditional alcoholic beverage made from the fermentation of Grewiaflava (mogwana) fruits (Figure 3). The beverage has been reported to contain an alcohol content of between 1.66% and 5.71% (v/v), with a pH from 2.87 to 3.16. The beverage has been compared favorably to commercial wine [56]. Brown sugar is added to the fruit juice before fermentation. One would posit that the sugar is required to aid fermentation of the juice or increase alcohol content. However, there is no available literature documenting the sugar content in the Grewia flava fruits. The other fruits used in khadi fermentation include Grewia occidentalis (moretlwa), Grewia flavascens (mokgomphatha), Kedrostis hirtella (mogakangwaga), and Khadia acutipetala [29]. The most well-known technique used in brewing khadi brewing is the back-slopping fermentation technique where an already fermented khadi beverage is used as a source of starter culture for the brewing. A Global Status Report on Alcohol by the World Health Organization (WHO) (2004) reported that the mixing in of mashed wild pumpkins, wild fruits, wild tuberous roots, can occur in the production of khadi [132].
Unorthodox production of this beverage has been known amongst the small scale producers to also include marijuana, car battery acid, tobacco, dagga, during preparation [26]. The reasons why such a harmful additives are used are not documented although some villagers claim that the battery acid increases intoxication [26]. The aromatic complexity of this beverage has been reported and it is said to contain 2-methyl-1-propanol, 2/3-methyl-1-butanol, ethyl lactate, and ethyl acetate [56]. These aromatic volatile compounds have also been reported in commercial beer, wines and spirits as flavor compounds [56]. Although the beverage is seemingly popular amongst Batswana, there is no literature available documenting the microbiological characteristics of khadi. The characterization of microbiological diversity and consequent chemical profiling of khadi will be important to ascertain the resultant variations of the beverage.

12.6. Setopoti

Setopoti is an alcoholic beverage made from watermelons (Citrullus lanatus). The watermelon pulp is collected in a clay pot which is then tightly closed. The preparation is then allowed to spontaneously ferment for 72 h undisturbed. After fermentation, the alcoholic beverage is sieved to remove seeds and solids, and served as a smooth fermented beverage. The alcoholic beverage is commonly consumed in the Central district of Botswana, more specifically in the Tswapong region. Watermelons that were not fresh (regarded as waste) were traditionally used to reduce post-harvest losses. Nowadays, fresh watermelons are also being used. The biochemical and microbiological diversity of setopoti is yet to be determined as there is no literature available regarding the alcoholic beverage.

12.7. Mukumbi

Mukumbi is a non-cereal-based alcoholic beverage consumed in many Zimbabwean villages as well as in semi-arid regions like Botswana. Mukumbi is traditionally prepared by spontaneously fermenting a mash prepared from ripe fruits of the marula plant (Sclerocarya birrea sub-species caffra) [133]. This alcoholic beverage is also produced and consumed in many SADC countries such as Namibia, Botswana, Swaziland, Zimbabwe and Zambia [9], and known by different names such as Omagongo in Namibia [57], buganu in Swaziland [134] and bojalwa-jwa-morula in Botswana. The marula fruits are peeled using a knife and put into a clay pot with the peels and pounded to extract the sugary juices. The juice is then allowed to spontaneously ferment for 3days. This suggests that the microflora from the fruit surfaces, the clay pots as well as from the hands of the handlers are involved in fermentation. Once fermentation is complete, the alcoholic beverage is sieved to remove the residual fruit skins. The product is then served with a final alcohol content of about 15% (v/v) [57].
The microorganisms isolated through culture-dependent methods from mukumbi include Saccharomyces cerevisiae, Pichiaanomala, P. guilliermondii, Candida tropicalis, and C. intermedia [101]. Aureobasidium pullulans, Geotrichumcapitatum, Trichosporon brassicae, Rhodotorula mucilaginosa, Hansenula anomala, H. jadinii, and other Hansenula species have been isolated from ripe marula fruits [124], and some are involved in the fermentation of the marula juice. The marula fruit naturally contains sucrose, fructose, and glucose, as such, the yeasts isolated from the mukumbi were tested to ascertain their ability to ferment these sugars. The results showed they (Saccharomyces cerevisiae, Pichiaanomala, P. guilliermondii, Candida tropicalis, and C. intermedia) could ferment glucose, galactose, maltose, sucrose, raffinose with an exception of P. guilliemondii which could not ferment galactose, raffinose, and maltose and, all the strains could not ferment lactose [101]. The unique sensory properties associated with this beverage are due to mixed culture fermentations, the unique tasting fruit and the resultant secondary metabolites that are yet to be fully elucidated.

12.8. Kachasu

Kachasu (also known as tototo, lukutu or nipa) is a traditionally fermented, highly intoxicating distilled alcoholic spirit with an alcohol content of 9% to 41% (v/v). Kachasu has been reported to be similar to waragi of Uganda and chang’aa of Kenya [5,127]. It is usually brewed using maize meal but bulrush or finger millet meal, various fruits such as masau (Ziziphusmauritiana), Adansoniadigitata, Tamarindusindica [37,133] and banana peels may be used as alternative sources of carbohydrates [5]. The carbohydrate source such as masau, is added to warm water in a pot with a hole drilled on the side, which is used later during the distillation of the spirit. The mixture is stirred into a slurry and allowed to simmer for a few minutes before the pot is removed from the fire. Sugar and yeast are added after the slurry has been cooled to ambient temperature. The hole in the pot is sealed with clay and the mixture allowed to ferment for 4to 7 days at ambient temperature.
The fermented brew is distilled over a small fire and the clear distillate is collected from the end of the pipe into bottles. Toxicity may be attributed to several co-generic alcohols such as isoamyl alcohol, iso-butanol, methanol, and other volatile compounds [5]. The other organic compounds, which have been identified in kachasu include acetaldehyde, acetone, ethyl acetate, and furfurals [5]. Kachasu production uses masau fruits (Ziziphus mauritiana) as a substrate which naturally contains microbes such as Saccharomyces cerevisiae, Issatchenkia orientalis, Pichia fabianii, Aureobasidium pullulans, Lactobacillus agilis, L. minor, L. confusus, L. fructosus, L. bifermentans, L. divergens, L. fermentum, L. hilgardii and Streptococcus spp. [9].

13. Plantain Beverages

Agadagidi is an alcoholic beverage produced from the fermentation of ripe plantain pulp (Musa paradisiaca) consumed in the South-western Nigeria and Cameroon, with a 6.57% (v/v) alcohol content and pH of 3.6 [92,102]. Agadagidi production involves packing, peeling, slicing of plantains/bananas in an earthenware pot, and covering with water. The pot is then covered tightly and the product allowed to ferment at room temperature for a period of l day to 5 days after which the juice is strained and the agadagidi is ready for consumption [102]. Plantain can also be used to produce wines with a pH from 4.1 to 4.4 and spirits with a pH from 2.8 to 3.3 [30]. The plantain wines are distilled and can have a reported final alcohol content of 47.4% (v/v) which is later decreased to 19.8% (v/v) [30]. The fermentation of agadagidi is carried out by S. cerevisiae, S. chevalieri, Lactococcuslactis, Bacillus subtilis, Brettanomyces intermedius, Kloekera apiculata, Candida tropicalis, C. krusei, Hansenula anomala, Pediococcus cerevisiae, L. mesenteroides, Staphylococcus epidermidis, and Micrococcus leteus [102,135]. There is no literature on the chemical characterization of these beverages.

14. Cassava Brews

Cassava (Manihotesculenta) is an important nutritional crop for many people in Africa [45]. Additional to its dietary consumption, cassava can be used as a substrate for fermentation. Cassava is used in the production of traditional spirits with an alcohol content of 7.8% to 26.3% (v/v) and a pH from 3.5 to 4.3 [30]. The production of alcoholic beverages from cassava is common in Arica as well as Brazil [136]. The cassava tuber is cleaned and soaked in water for 7 days. After 7 days, the tubers are peeled and grinded to form a pulp which then has water added to it before being left for 3 days to ferment [137]. Upon completion of fermentation, the product is sieved to remove any solids, and the filtrate is ready for consumption. Traditional production of cassava spirits uses cassava flour, sorghum grains, and baker’s yeast. The process follows the steps of sorghum beer production (malting, souring, boiling, mashing, and straining) but using cassava flour and sorghum flour. The fermentation is carried out for 4 days by baker’s yeast and the final product is distilled using a drum at the outlet pipe sank is cold water [137]. The chemical and microbial diversity of traditional African alcoholic cassava brews is yet to be performed and documented.
In the Brazilian beverage caxiri (produced from cassava and sweet potatoes), researchers isolated Saccharomyces cerevisiae, Pichia membranifaciens, P. guilliermondii, Cryptococcus luteolus, Sphingomonas spp., Pediococcus acidilactici, Bacillus pumilus and B. subtilis [138]. In another Brazilian beverage namely, taruba, they isolated L. plantarum, L. brevis, L. mesenteroides, B. subtilis, Torulaspora delbrueckii, P. exigua, Candida rugosa, C. tropicalis, P. kudriavzevii, Wickerhamomyces anomalus, C. ethanolica, Bacillus amyloliquefaciens, B. licheniformis, Hanseniaspora uvarum, and Chitinophaga terrae [136].
Cassava in the form of gari (a starchy powder produced from cassava) is added to the sorghum malt during the production of burukutu as an adjunct [87]. Adjuncts are starch or sugar containing materials added in addition to the carbohydrates in the malt to boost the level of fermentable carbohydrate. Burukutu (brown colored suspension with a vinegar-like flavor) is an indigenous alcoholic beverage produced and consumed in the Republic of Benin, Ghana, Northern Guinea and Savannah region of Nigeria [139]. The substrates are fermented by Saccharomy cescerevisiae, S. chevalieri and Leuconostoc mesteroides [6].

15. Conclusions

Non-cereal carbohydrate substrates for the production of alcoholic beverages are an attractive commercialization initiative. Although cereal-based beverages are well known in different parts of the African continent, there is an immense diversity of alcoholic beverages made from non-cereal fermentable substrates in the sub-Saharan African region. A limitation is that non-cereal-based alcoholic beverages are less documented, although they exhibit very rich aromatic properties that are attractive for commercialization. Their unique sensory properties could be largely influenced by a rich microbial diversity in the sub-Saharan region which stems from its well-known undisturbed ecosystems. Such an immense microbial biodiversity in the region is applicable for use as fermentation consortia. The lack of robust documentation and minimal exploration remains an untapped potential of the sub-Saharan region.
Diverse microbial species with a preference for different fermentable sugars and ecological niches result in distinct aromatic and flavor profiles. Effort needs to be directed towards ascertaining the interplay of particular combinations of aromatic molecules produced by microbial consortia as it is a major factor for the production of distinctively flavored beverages. Equally significant, is the climatic differences amongst the Sub-Saharan countries which could also be an added advantage as such variances affects wild and cultivated fruit, tree sap, honey, root and tuber sugar compositions and hence leads to differential sensory profiles of beverages. The commercialization of such beverages in the modern Africa could be of much needed economic importance. The increased demand for a variety of beverages and lifestyles clearly calls for the scientific community to work towards meeting consumer demands. The use of mixed consortia which produce metabolites such acetic acid and lactic acid and other natural food preservatives which prolong the shelf life of fermented traditional alcoholic beverages and reduce the use of chemical food preservatives is an added advantage.
The mining of literature, the harnessing of traditional beverage processing knowledge among diverse communities and conducting research to document the microbial and chemical profiles of non-cereal-based alcoholic traditional beverages of the Sub-Saharan region is important for establishment of knowledge towards commercialization of non-cereal based alcoholic beverages.

Conflicts of Interest

The authors declare no conflict of interest.

References

  1. Sekwati-Monang, B.; Gänzle, M.G. Microbiological and chemical characterisation of ting, a sorghum-based sourdough product from Botswana. Int. J. Food Microbiol. 2011, 150, 115–121. [Google Scholar] [CrossRef] [PubMed]
  2. Sharma, A.; Kapoor, A. Levels of antinutritional factors in pearl millet as affected by processing treatments and various types of fermentation. Plant Foods Hum. Nutr. 1996, 49, 241–252. [Google Scholar] [CrossRef] [PubMed]
  3. Devi, P.B.; Vijayabharathi, R.; Sathyabama, S. Health benefits of finger millet (Eleusine coracana L.) polyphenols and dietary fiber: A review. Nutr. Food Sci. 2014, 51, 1021–1040. [Google Scholar] [CrossRef] [PubMed]
  4. Gimbi, D.M.; Kitabatake, N. Changes in alpha-and beta-amylase activities during seed germination of African finger millet. Int. J. Food Sci. Nutr. 2002, 53, 481–488. [Google Scholar] [CrossRef] [PubMed]
  5. Gadaga, T.H.; Mutukumira, A.N.; Narvhus, J.A.; Feresu, S.B. A review of traditional fermented foods and beverages of Zimbabwe. Int. J. Food Microbiol. 1999, 53, 1–11. [Google Scholar] [CrossRef]
  6. Kolawole, O.M.; Kayode, R.M.O.; Akinduyo, B. Proximate and microbial analyses of burukutu and pito produced in Ilorin, Nigeria. Afr. J. Biotechnol. 2007, 6, 587–590. [Google Scholar]
  7. Lee, M.; Regu, M.; Seleshe, S. Uniqueness of Ethiopian traditional alcoholic beverage of plant origin, tella. J. Ethn. Foods 2015, 2, 110–114. [Google Scholar] [CrossRef]
  8. Onyenekwe, P.C.; Erhabor, G.O.; Akande, S.A. Characterisation of aroma volatiles of indigenous alcoholic beverages: Burukutu and pito. Nat. Prod. Res. 2016, 30, 558–564. [Google Scholar] [CrossRef] [PubMed]
  9. Nyanga, L.K.; Nout, M.J.R.; Gadaga, T.H.; Theelen, B.; Boekhout, T.; Zwietering, M.H. Yeasts and lactic acid bacteria microbiota from masau (Ziziphus mauritiana) fruits and their fermented fruit pulp in Zimbabwe. Int. J. Food Microbiol. 2007, 120, 159–166. [Google Scholar] [CrossRef] [PubMed]
  10. Chelule, P.; Mokoena, M.; Gqaleni, N. Advantages of traditional lactic acid bacteria fermentation of food in Africa. Curr. Res. Technol. Educ. Top. Appl. Microbiol. Microb. Biotechnol. 2010, 2, 1160–1167. [Google Scholar]
  11. Tamang, J.P.; Watanabe, K.; Holzapfel, W.H. Review: Diversity of microorganisms in global fermented foods and beverages. Front. Microbiol. 2016, 7. [Google Scholar] [CrossRef] [PubMed]
  12. Tafere, G. A review on Traditional Fermented Beverages of Ethiopian. J. Nat. Scinces Res. 2015, 5, 94–103. [Google Scholar]
  13. Blandino, A.; Al-Aseeri, M.E.; Pandiella, S.S.; Cantero, D.; Webb, C. Cereal-based fermented foods and beverages. Food Res. Int. 2003, 36, 527–543. [Google Scholar] [CrossRef]
  14. Jane, M.M.; Lodewyk, K.; Elma, P.; Carlien, P.; Remigio, Z. Characterisation of yeasts isolated from traditional opaque beer beverages brewed in Zimbabwean households. Afr. J. Microbiol. Res. 2015, 9, 549–556. [Google Scholar] [CrossRef]
  15. Escalante, A.; Giles-Gómez, M.; Hernández, G.; Córdova-Aguilar, M.S.; López-Munguía, A.; Gosset, G.; Bolívar, F. Analysis of bacterial community during the fermentation of pulque, a traditional Mexican alcoholic beverage, using a polyphasic approach. Int. J. Food Microbiol. 2008, 124, 126–134. [Google Scholar] [CrossRef] [PubMed]
  16. Figueroa, L.M.; Gutiérrez-Uribe, J.A.; Martín-del-Campo, S.T.; Escalante, A. Fermented Foods in Health and Disease Prevention; Frias, J., Martinez-Villaluenga, C., Penas, E., Eds.; Academic Press: London, UK, 2017; ISBN 9780128023099. [Google Scholar]
  17. Escalante, A.; Soto, D.R.L.; Gutiérrez, J.E.V.; Giles-gómez, M.; Bolívar, F.; López-munguía, A. Pulque, a Traditional Mexican Alcoholic Fermented Beverage: Historical, Microbiological, and Technical Aspects. Front. Microbiol. 2016, 7, 1–18. [Google Scholar] [CrossRef] [PubMed]
  18. Oliveira, A.; Arau, M.; Fietto, L.G.; Castro, I.D.M.; Coutrim, X.; Schu, D.; Alves, H.; Casal, M.; Santos, J.D.O.; Henrique, P. Biochemical and Molecular Characterization of Saccharomyces cerevisiae Strains Obtained from Sugar-Cane Juice Fermentations and Their Impact in Cachaca. Appl. Environ. Microbiol. 2008, 74, 693–701. [Google Scholar] [CrossRef] [PubMed]
  19. Franco, G.R.; Mourão, M.M.; Rosa, C.A. Identification of lactic acid bacteria associted with traditional cachaca fermentation. Braz. J. Microbiol. 2010, 41, 486–492. [Google Scholar]
  20. Behera, S.K. Kefir and Koumiss Origin. In Fermented Food—Part II: Technological Interventions; CRC Press: Boca Raton, FL, USA, 2017; pp. 401–417. [Google Scholar]
  21. Rosa, D.D.; Dias, M.M.S.; Grześkowiak, Ł.M.; Reis, S.A.; Conceição, L.L.; do Peluzio, M.C.G. Milk kefir: Nutritional, microbiological and health benefits. Nutr. Res. Rev. 2017, 30, 82–96. [Google Scholar] [CrossRef] [PubMed]
  22. Mu, Z.; Yang, X.; Yuan, H. Detection and identification of wild yeast in Koumiss. Food Microbiol. 2012, 31, 301–308. [Google Scholar] [CrossRef] [PubMed]
  23. Azam, M.; Mohsin, M.; Ijaz, H.; Tulain, U.R.; Ashraf, M.A.; Fayyaz, A.; Abadeen, Z.; Kamran, Q. Lactic acid bacteria in traditional fermented Chinese foods. Food Res. Int. 2011, 44, 643–651. [Google Scholar] [CrossRef]
  24. Shamala, T.R.; Sreekantiah, K.R. Microbiological and biochemical studies on traditional Indian palm wine fermentation. Food Microbiol. 1988, 5, 157–162. [Google Scholar] [CrossRef]
  25. Choi, S.-H. Characterization of airag collected in Ulaanbaatar, Mongolia with emphasis on isolated lactic acid bacteria. J. Anim. Sci. Technol. 2016, 58, 10. [Google Scholar] [CrossRef] [PubMed]
  26. Von Rudloff, A.; Mogkatlhe, L.; Mookodi, G. Botswana: Summary of nationwide research, in International Center for Alcohol Policies (ed.), Producers, Sellers, and Drinkers: Studies of Noncommercial Alcohol in Nine Countries. In Global Actions on Harmful Drinking; ICAP Monograph: Washington, DC, USA, 2012; pp. 13–18. [Google Scholar]
  27. Bahiru, B.; Mehari, T.; Ashenafi, M. Yeast and lactic acid flora of tej, an indigenous Ethiopian honey wine: Variations within and between production units. Food Microbiol. 2006, 23, 277–282. [Google Scholar] [CrossRef] [PubMed]
  28. Yohannes, T.; Melak, F.; Siraj, K. Preparation and physicochemical analysis of some Ethiopian traditional alcoholic beverages. Afr. J. Food Sci. 2013, 7, 399–403. [Google Scholar] [CrossRef]
  29. Van Wyk, B.E.; Gericke, N. People’s Plants: A Guide to Useful Plants of Southern Africa; Briza Publications: Johannesburg, South Africa, 2000. [Google Scholar]
  30. Kubo, R.; Funakawa, S.; Araki, S.; Kitabatake, N. Production of indigenous alcoholic beverages in a rural village of Cameroon. J. Inst. Brewing. 2014, 133–141. [Google Scholar] [CrossRef]
  31. Miguel, J.; Tulipani, A.S.; Romandini, S.; Bertoli, E.; Battino, M. Contribution of honey in nutrition and human health: A review. Mediterr. J. Nutr. Metab. 2010, 3, 15–23. [Google Scholar] [CrossRef]
  32. Olaitan, P.B.; Adeleke, O.E. Honey: A reservoir for microorganisms and an inhibitory. Afr. Health Sci. 2007, 7, 159–165. [Google Scholar]
  33. Bogdanov, S.; Jurendic, T.; Sieber, R. Honey for Nutrition and Health: A Review. Am. J. Coll. Nutr. 2008, 27, 677–689. [Google Scholar] [CrossRef]
  34. Amoa-Awua, W.K.; Sampson, E.; Tano-Debrah, K. Growth of yeasts, lactic and acetic acid bacteria in palm wine during tapping and fermentation from felled oil palm (Elaeis guineensis) in Ghana. J. Appl. Microbiol. 2007, 102, 599–606. [Google Scholar] [CrossRef] [PubMed]
  35. Santiago-Urbina, J.A.; Rui’z-Tera’n, F. Microbiology and biochemistry of traditional palm wine produced around the world. Int. Food Res. J. 2014, 21, 1261–1269. [Google Scholar]
  36. Makhlouf-gafsi, I.; Mokni-ghribi, A.; Bchir, B.; Attia, H.; Blecker, C.; Besbes, S. Physico-chemical properties and amino acid profiles of sap from Tunisian date palm. Sci. Agric. 2016, 73, 85–90. [Google Scholar] [CrossRef]
  37. Nyanga, L.K.; Nout, M.J.R.; Gadaga, T.H.; Boekhout, T.; Zwietering, M.H. Traditional processing of masau fruits (Ziziphus mauritiana) in Zimbabwe. Ecol. Food Nutr. 2008, 47, 95–107. [Google Scholar] [CrossRef]
  38. Dellacassa, E.; Trenchs, O.; Fariña, L.; Debernardis, F.; Perez, G.; Boido, E.; Carrau, F. Pineapple (Ananas comosus L. Merr.) wine production in Angola: Characterisation of volatile aroma compounds and yeast native flora. Int. J. Food Microbiol. 2017, 241, 161–167. [Google Scholar] [CrossRef] [PubMed]
  39. Haruenkit, R. Analysis of sugars and organic acids in pineapple, papaya and star fruit by HPLC using an Aminex HPx-87 H column. J. Anal. Chem. 1988, 22, 11–18. [Google Scholar]
  40. Makebe, C.W.; Desobgo, Z.S.C.; Nso, E.J. Optimization of the Juice Extraction Process and Investigation on Must Fermentation of Overripe Giant Horn Plantains. Beverages 2017, 3, 19. [Google Scholar] [CrossRef]
  41. Ketiku, O.A. Chemical composition of unripe (green) and ripe plantain (Musa paradiasca). J. Sci. Food Agric. 1973, 24, 703–707. [Google Scholar] [CrossRef] [PubMed]
  42. Wilson, P.; David, T.; Sam, B. Microbial and Biochemical Changes Occurring during Production of Traditional Rwandese Banana Beer “Urwagwa”. Ferment. Technol. 2015, 1, 1–5. [Google Scholar] [CrossRef]
  43. Pareek, S. Nutritional Composition of Fruit Cultivars; Simmonds, M., Preed, V., Eds.; Academic Press: London, UK, 2016; ISBN 978-0-12-408117-8. [Google Scholar]
  44. Colehour, A.M.; Meadow, J.F.; Liebert, M.A.; Cepon-robins, T.J.; Gildner, T.E.; Urlacher, S.S.; Bohannan, B.J.M.M.; Snodgrass, J.J.; Sugiyama, L.S. Local domestication of lactic acid bacteria via cassava beer fermentation. PeerJ 2014, 2, e479. [Google Scholar] [CrossRef] [PubMed]
  45. Coulin, P.; Farah, Z.; Assanvo, J.; Spillmann, H.; Puhan, Z. Characterisation of the microflora of attieke, a fermented cassava product, during traditional small-scale preparation. Int. J. Food Microbiol. 2006, 106, 131–136. [Google Scholar] [CrossRef] [PubMed]
  46. Freire, A.L.; Ramos, C.L.; Schwan, R.F. Microbiological and chemical parameters during cassava based-substrate fermentation using potential starter cultures of lactic acid bacteria and yeast. Food Res. Int. 2015, 76, 787–795. [Google Scholar] [CrossRef] [PubMed]
  47. Salvador, E.M.; Steenkamp, V.; McCrindle, C.M.E. Production, consumption and nutritional value of cassava (Manihot esculenta, Crantz) in Mozambique: An overview. J. Agric. Biotechnol. Sustain. Dev. 2014, 6, 29–38. [Google Scholar] [CrossRef]
  48. Afoakwa, E.O.; Budu, A.S.; Asiedu, C.; Chiwona-karltun, L.; Nyirenda, D.B. Nutrition & Food Application of Multivariate Techniques for Characterizing Composition of Starches and Sugars in Six High Yielding CMD Resistant Cassava (Manihot esculenta Crantz) Varieties. Nutr. Food Sci. 2011, 1, 1–5. [Google Scholar] [CrossRef]
  49. Mariod, A.A.; Abdelwahab, S.I. Sclerocarya birrea (Marula), an African Tree of Nutritional and Medicinal Uses: A Review. Food Rev. Int. 2012, 28, 375–388. [Google Scholar] [CrossRef]
  50. Dlamini, N.R.; Dube, S. Studies on the physico-chemical, nutritional and microbiological changes during the traditional preparation of Marula wine in Gwanda, Zimbabwe. Nutr. Food Sci. 2008. [Google Scholar] [CrossRef]
  51. Hal, P.H.; George, P. A review of the proximate composition and nutritional value of Marula (Sclerocarya birrea subsp. caffra). Phytochem. Rev. 2014, 13, 881–892. [Google Scholar] [CrossRef]
  52. Teshome, D.A.; Rainer, M.; Gerhard, J.N.; Fuchs, S.D.; Bliem, H.R.; Günther, B.K. Chemical compositions of traditional alcoholic beverages and consumers’ characteristics, Ethiopia. Afr. J. Food Sci. 2017, 11, 234–245. [Google Scholar] [CrossRef]
  53. Shale, K.; Mukamugema, J.; Lues, R.J.; Venter, P.; Mokoena, K.K. Characterisation of selected volatile organic compounds in Rwandan indigenous beer “Urwagwa” by dynamic headspace gas chromatography-mass spectrometry. Afr. J. Biotechnol. 2013, 12, 2990–2996. [Google Scholar] [CrossRef]
  54. Nwachukwu, I.; Ekaiko, M.U.; Stephen, C. Microbiological quality of palm wine (Elaeis guineensis and Raphia hookeri) sold within Aba Metropolis, Abia State, South Eastern Nigeria. Eur. J. Biotechnol. Genet. Eng. 2016, 3, 38–44. [Google Scholar]
  55. Steinkraus, H.K. Fermentations in world food processing. Compr. Rev. Food Sci. Food Saf. 2002, 1, 23–32. [Google Scholar] [CrossRef]
  56. Mapitse, R.; Okatch, H.; Moshoeshoe, E. Analysis of Volatile Compounds in Khadi (an Unrecorded Alcohol Beverage) from Botswana by Gas Chromatography-Flame Ionization Detection (GC-FID). S. Afr. J. Chem. 2014, 67, 184–188. [Google Scholar]
  57. Misihairabgwi, J.; Cheikhyoussef, A. Traditional fermented foods and beverages of Namibia. J. Ethn. Foods 2017, 1–9. [Google Scholar] [CrossRef]
  58. Ayogu, T.E. Evaluation of the performance of a yeast isolate from Nigerian palm wine in wine production from pineapple fruits. Bioresour. Technol. 1999, 69, 189–190. [Google Scholar] [CrossRef]
  59. Roda, A.; Lucini, L.; Torchio, F.; Dordoni, R.; Marco, D.; Faveri, D.; Lambri, M. Metabolite profiling and volatiles of pineapple wine and vinegar obtained from pineapple waste. Food Chem. 2017, 229, 734–742. [Google Scholar] [CrossRef] [PubMed]
  60. Pino, J.A.; Queris, O. Analysis of volatile compounds of pineapple wine using solid-phase microextraction techniques. Food Chem. 2010, 122, 1241–1246. [Google Scholar] [CrossRef]
  61. Canonico, L.; Comitini, F.; Ciani, M. Torulaspora delbrueckii contribution in mixed brewing fermentations with different Saccharomyces cerevisiae strains. Int. J. Food Microbiol. 2017, 259, 7–13. [Google Scholar] [CrossRef] [PubMed]
  62. Marshall, E.; Mejia, D. Traditional Fermented Food and Beverages for Improved Livelihoods; FAO: Rome, Italy, 2011; ISBN 9789251070741. [Google Scholar]
  63. Dashko, S.; Zhou, N.; Tinta, T.; Sivilotti, P. Use of non-conventional yeast improves the wine aroma profile of Ribolla Gialla. J. Ind. Microbiol. Biotechnol. 2015, 997–1010. [Google Scholar] [CrossRef] [PubMed]
  64. Achi, O.K. The potential for upgrading traditional fermented foods through biotechnology. Afr. J. Biotechnol. 2005, 4, 375–380. [Google Scholar]
  65. Lencioni, L.; Romani, C.; Gobbi, M.; Comitini, F.; Ciani, M.; Domizio, P. Controlled mixed fermentation at winery scale using Zygotorulaspora florentina and Saccharomyces cerevisiae. Int. J. Food Microbiol. 2016, 234, 36–44. [Google Scholar] [CrossRef] [PubMed]
  66. Dashko, S.; Zhou, N.; Compagno, C.; Piskur, J. Why, when, and how did yeast evolve alcoholic fermentation? FEMS Yeast Res. 2014, 14, 826–832. [Google Scholar] [CrossRef] [PubMed]
  67. Walker, G.; Stewart, G. Saccharomyces cerevisiae in the Production of Fermented Beverages. Beverages 2016, 2, 30. [Google Scholar] [CrossRef]
  68. Garofalo, C.; Arena, M.P.; Laddomada, B.; Cappello, M.S.; Bleve, G.; Grieco, F.; Beneduce, L.; Berbegal, C.; Spano, G.; Capozzi, V. Starter Cultures for Sparkling Wine. Fermentation 2016, 2, 21. [Google Scholar] [CrossRef]
  69. Drumonde-Neves, J.; Franco-Duarte, R.; Lima, T.; Schuller, D.; Pais, C. Association between grape yeast communities and the vineyard ecosystems. PLoS ONE 2017, 12, e0169883. [Google Scholar] [CrossRef] [PubMed]
  70. Barata, A.; Malfeito-Ferreira, M.; Loureiro, V. The microbial ecology of wine grape berries. Int. J. Food Microbiol. 2012, 153, 243–259. [Google Scholar] [CrossRef] [PubMed]
  71. De Ponzzes-Gomes, C.M.P.B.S.; de Mélo, D.L.F.M.; Santana, C.A.; Pereira, G.E.; Mendonça, M.O.C.; Gomes, F.C.O.; Oliveira, E.S.; Barbosa, A.M.; Trindade, R.C.; Rosa, C.A. Saccharomyces cerevisiae and non-Saccharomyces yeasts in grape varieties of the São Francisco Valley. Braz. J. Microbiol. 2014, 45, 411–416. [Google Scholar] [CrossRef] [PubMed]
  72. Swiegers, J.H.; Kievit, R.L.; Siebert, T.; Lattey, K.A.; Bramley, B.R.; Francis, I.L.; King, E.S.; Pretorius, I.S. The influence of yeast on the aroma of Sauvignon Blanc wine. Food Microbiol. 2009, 26, 204–211. [Google Scholar] [CrossRef] [PubMed]
  73. Schifferdecker, A.J.; Dashko, S.; Ishchuk, O.P.; Piškur, J. The wine and beer yeast Dekkera bruxellensis. Yeast 2014, 31, 323–332. [Google Scholar] [CrossRef] [PubMed]
  74. Varela, C. The impact of non-Saccharomyces yeasts in the production of alcoholic beverages. Appl. Microbiol. Biotechnol. 2016, 9861–9874. [Google Scholar] [CrossRef] [PubMed]
  75. Pretorius, I.S. Tailoring wine yeast for the new millennium: Novel approaches to the ancient art of winemaking. Yeast 2000, 16, 675–729. [Google Scholar] [CrossRef]
  76. Hansen, E.H.; Nissen, P.; Sommer, P.; Nielsen, J.C.; Arneborg, N.; Di Maro, E.; Ercolini, D.; Coppola, S.; Pretorius, I.S. The effect of oxygen on the survival of non-Saccharomyces yeasts during mixed culture fermentations of grape juice with Saccharomyces cerevisiae. J. Appl. Microbiol. 2001, 91, 541–547. [Google Scholar] [CrossRef]
  77. Di Maro, E.; Ercolini, D.; Coppola, S. Yeast dynamics during spontaneous wine fermentation of the Catalanesca grape. Int. J. Food Microbiol. 2007, 117, 201–210. [Google Scholar] [CrossRef] [PubMed]
  78. Hagman, A.; Sall, T.; Compagno, C.; Piskur, J. Yeast “Make-Accumulate-Consume” Life Strategy Evolved as a Multi-Step Process That Predates the Whole Genome Duplication. PLoS ONE 2013, 8. [Google Scholar] [CrossRef] [PubMed]
  79. Zhou, N.; Swamy, K.B.S.; Leu, J.Y.; McDonald, M.J.; Galafassi, S.; Compagno, C.; Piškur, J. Coevolution with bacteria drives the evolution of aerobic fermentation in Lachancea kluyveri. PLoS ONE 2017, 12. [Google Scholar] [CrossRef] [PubMed]
  80. De Deken, R.H. The Crabtree Effect: A Regulatory System in Yeast. J. Gen. Microbiol. 1966, 44, 149–156. [Google Scholar] [CrossRef] [PubMed]
  81. Stringini, M.; Comitini, F.; Taccari, M.; Ciani, M. Yeast diversity during tapping and fermentation of palm wine from Cameroon. Food Microbiol. 2009, 26, 415–420. [Google Scholar] [CrossRef] [PubMed]
  82. Lee, Y.J.; Choi, Y.R.; Lee, S.Y.; Park, J.T.; Shim, J.H.; Park, K.H.; Kim, J.W. Screening wild yeast strains for alcohol fermentation from various fruits. Mycobiology 2011, 39, 33–39. [Google Scholar] [CrossRef] [PubMed]
  83. Lleixà, J.; Manzano, M.; Mas, A.; del Portillo, M.C. Saccharomyces and non-Saccharomyces competition during microvinification under different sugar and nitrogen conditions. Front. Microbiol. 2016, 7. [Google Scholar] [CrossRef] [PubMed]
  84. Yang, S.; Fei, Q.; Zhang, Y.; Contreras, L.M.; Utturkar, S.M.; Brown, S.D.; Himmel, M.E.; Zhang, M. Zymomonas mobilis as a model system for production of biofuels and biochemicals. Microb. Biotechnol. 2016, 9, 699–717. [Google Scholar] [CrossRef] [PubMed]
  85. He, M.; Wu, B.; Qin, H.; Ruan, Z.; Tan, F.; Wang, J.; Shui, Z.; Dai, L.; Zhu, Q.; Pan, K.; et al. Zymomonas mobilis: A novel platform for future biorefineries. Biotechnol. Biofuels 2014, 7, 101. [Google Scholar] [CrossRef] [PubMed]
  86. Obire, O. Activity of Zymomonas species in palm-sap obtained from three areas in Edo State, Nigeria. J. Appl. Sci. Environ. Manag. 2005, 9, 25–30. [Google Scholar]
  87. Lyumugabe, F.; Gros, J.; Nzungize, J.; Bajyana, E.; Thonart, P. Characteristics of African traditional beers brewed with sorghum malt: A review. Biotechnol. Agron. Soc. Environ. 2012, 16, 509–530. [Google Scholar]
  88. Lütke-Eversloh, T.; Bahl, H. Metabolic engineering of Clostridium acetobutylicum: Recent advances to improve butanol production. Curr. Opin. Biotechnol. 2011, 22, 634–647. [Google Scholar] [CrossRef] [PubMed]
  89. Sreekumar, S.; Baer, Z.C.; Pazhamalai, A.; Gunbas, G.; Grippo, A.; Blanch, H.W.; Clark, D.S.; Toste, F.D. Production of an acetone-butanol-ethanol mixture from Clostridium acetobutylicum and its conversion to high-value biofuels. Nat. Protoc. 2015, 10, 528–537. [Google Scholar] [CrossRef] [PubMed]
  90. Guo, X.; Cao, C.; Wang, Y.; Li, C.; Wu, M.; Chen, Y.; Zhang, C.; Pei, H.; Xiao, D. Effect of the inactivation of lactate dehydrogenase, ethanol dehydrogenase, and phosphotransacetylase on 2,3-butanediol production in Klebsiella pneumoniae strain. Biotechnol. Biofuels 2014, 7, 1–11. [Google Scholar] [CrossRef] [PubMed]
  91. Tamang, J.P.; Shin, D.H.; Jung, S.J.; Chae, S.W. Functional properties of microorganisms in fermented foods. Front. Microbiol. 2016, 7, 1–13. [Google Scholar] [CrossRef] [PubMed]
  92. Jespersen, L. Occurrence and taxonomic characteristics of strains of Saccharomyces cerevisiae predominant in African indigenous fermented foods and beverages. FEMS Yeast Res. 2003, 3, 191–200. [Google Scholar] [CrossRef]
  93. Barukčić, I.; Gracin, L.; Jambrak, A.R.; Božanić, R. Comparison of chemical, rheological and sensory properties of kefir produced by kefir grains and commercial kefir starter. Mljekarstvo 2017, 67, 163–176. [Google Scholar] [CrossRef]
  94. Muyanja, C.M.B.K.; Narvhus, J.A.; Treimo, J.; Langsrud, T. Isolation, characterisation and identification of lactic acid bacteria from bushera: A Ugandan traditional fermented beverage. Food Microbiol. 2003, 80, 201–210. [Google Scholar] [CrossRef]
  95. Kamaruzaman, M.R.B. Isolation of Microorganism from oil Palm Sap; University of Malaysia Pahang: Gambang, Malaysia, 2011. [Google Scholar]
  96. Hittinger, C.T. Saccharomyces diversity and evolution: A budding model genus. Trends Genet. 2013, 29, 309–317. [Google Scholar] [CrossRef] [PubMed]
  97. Jeffries, T.; Hittinger, C.T.; Rokas, A.; Bai, F.; Boekhout, T.; Gonc, P.; Jeffries, T.W.; Libkind, D.; Sampaio, P.; Kurtzman, C.P.; Rosa, C.A. Genomics and the making of yeast biodiversity. Genet. Dev. 2015, 35, 100–109. [Google Scholar] [CrossRef]
  98. Nwakakanma, C.; Unachukwu, N.M.; Onah, P.; Engwa, A.G. Isolation and sensory evaluation of Saccharomyces cerevisiae from palm wine (Elaeis guinneensis) gotten from different sites in Enugu. Eur. J. Biomed. Pharm. Sci. 2015, 2, 19–26. [Google Scholar]
  99. Shale, K.; Mukamugema, J.; Lues, R.J.; Venter, P. Possible microbial and biochemical contaminants of an indigenous banana beer “Urwagwa”: A mini review. Afr. J. Food Sci. Rev. 2014, 8, 376–389. [Google Scholar] [CrossRef]
  100. Fleet, G.H. Yeasts in foods and beverages: Impact on product quality and safety. Food Biotechnol. 2007, 18, 170–175. [Google Scholar] [CrossRef] [PubMed]
  101. Mpofu, A.; Pretorious, E.E.; Pohl, C.H.; Zvauya, R. Identification of yeasts isolated from Mukumbi, a Zimbabwe traditional wine. J. Sustain. Dev. Africa 2008, 10, 88–102. [Google Scholar]
  102. Iwuoha, C.I.; Eke, O.S. Nigerian indigenous fermented foods: Their traditional process operation, inherent problems, improvements and current status. Food Res. Int. 1996, 29, 527–540. [Google Scholar] [CrossRef]
  103. Brock, T.D. The study of microorganisms in situ: Progress and problems. In Symposium of the Society for General Microbiology; Cambridge University Press: New York, NY, USA, 1987; Volume 41, pp. 1–17. [Google Scholar]
  104. Wade, W. Unculturable bacteria: The uncharacterized organisms that cause oral infections. J. R. Soc. Med. 2002, 95, 81–83. [Google Scholar] [PubMed]
  105. Styger, G.; Prior, B.; Bauer, F.F. Wine flavor and aroma. J. Ind. Microbiol. Biotechnol. 2011, 38, 1145–1159. [Google Scholar] [CrossRef] [PubMed]
  106. Kong, Q.X.; Zhang, A.L.; Cao, L.M.; Chen, X. Over-expressing GLT1 in a gpd2∆ mutant of Saccharomyces cerevisiae to improve ethanol production. Appl. Microbiol. Biotechnol. 2007, 75, 1361–1366. [Google Scholar] [CrossRef] [PubMed]
  107. Navarrete, C.; Nielsen, J.; Siewers, V. Enhanced ethanol production and reduced glycerol formation in fps1∆ mutants of Saccharomyces cerevisiae engineered for improved redox balancing. AMB Express 2014, 4, 2–8. [Google Scholar] [CrossRef] [PubMed]
  108. Dussap, C.G.; Poughon, L. Microbiology of Alcoholic Fermentation. In Current Developments in Biotechnology and Bioengineering: Food and Beverages Industry; Marinakis, K., Ed.; Elsevier B.V.: Amsterdam, The Netherlands, 2016; pp. 263–279. ISBN 978-0-444-63666-9. [Google Scholar]
  109. Carrau, F.M.; Medina, K.; Farina, L.; Boido, E.; Henschke, P.A.; Dellacassa, E. Production of fermentation aroma compounds by Saccharomyces cerevisiae wine yeasts: Effects of yeast assimilable nitrogen on two model strains. FEMS Yeast Res. 2008, 8, 1196–1207. [Google Scholar] [CrossRef] [PubMed]
  110. Pino, J.A.; Queris, O. Analysis of volatile compounds of mango wine. Food Chem. 2011, 125, 1141–1146. [Google Scholar] [CrossRef]
  111. Benés, I.; Furdíková, K.; Šmogrovičová, D. Influence of Saccharomyces cerevisiae strain on the profile of volatile organic compounds of blossom honey mead. Czech. J. Food Sci. 2015, 33, 334–339. [Google Scholar] [CrossRef]
  112. Michel, M.; Meier-Dörnberg, T.; Jacob, F.; Methner, F.J.; Wagner, R.S.; Hutzler, M. Review: Pure non-Saccharomyces starter cultures for beer fermentation with a focus on secondary metabolites and practical applications. J. Inst. Brew. 2016, 122, 569–587. [Google Scholar] [CrossRef]
  113. Karamoko, D.; Djeni, N.T.; N’guessan, K.F.; Bouatenin, K.M.J.P.; Dje, K.M. The biochemical and microbiological quality of palm wine samples produced at different periods during tapping and changes which occured during their storage. Food Control 2012, 26, 504–511. [Google Scholar] [CrossRef]
  114. Magalhães, K.T.; Dragone, G.; De Melo Pereira, G.V.; Oliveira, J.M.; Domingues, L.; Teixeira, J.A.; E Silva, J.B.A.; Schwan, R.F. Comparative study of the biochemical changes and volatile compound formations during the production of novel whey-based kefir beverages and traditional milk kefir. Food Chem. 2011, 126, 249–253. [Google Scholar] [CrossRef]
  115. Arslan, M.M.; Zeren, C.; Aydin, Z.; Akcan, R.; Dokuyucu, R.; Keten, A.; Cekin, N. Journal of Forensic and Legal Medicine Analysis of methanol and its derivatives in illegally produced alcoholic beverages. J. Forensic Leg. Med. 2015, 33, 56–60. [Google Scholar] [CrossRef] [PubMed]
  116. Aloys, N.; Angeline, N. Traditional fermented foods and beverages in Burundi. Food Res. Int. 2009, 42, 588–594. [Google Scholar] [CrossRef]
  117. Manel, Z.; Sana, M.; Nedia, K.; Moktar, H.; Ali, F. Microbiological analysis and screening of lactic acid bacteria from Tunisian date palm sap. Afr. J. Microbiol. Res. 2011, 5, 2929–2935. [Google Scholar] [CrossRef]
  118. Regodón Mateos, J.A.; Pérez-Nevado, F.; Ramírez Fernández, M. Influence of Saccharomyces cerevisiae yeast strain on the major volatile compounds of wine. Enzyme Microb. Technol. 2006, 40, 151–157. [Google Scholar] [CrossRef]
  119. Naydenova, V.; Iliev, V.; Kaneva, M.; Kostov, G.; Koprinkova-Hristova, P.; Popova, S. Modeling of alcohol fermentation in brewing—Carbonyl compounds synthesis and reduction. In Proceedings of the 28th European Conference on Modelling and Simulation (ECMS 2014), Brescia, Italy, 27–30 May 2014; pp. 279–284. [Google Scholar]
  120. Pereira, A.P.; Oliveira, J.M.; Mendes-Ferreira, A.; Estevinho, L.M.; Mendes-Faia, A. Mead and Other Fermented Beverages. In Current Developments in Biotechnology and Bioengineering: Food and Beverages Industry; Marinakis, K., Ed.; Elsevier B.V.: Amsterdam, The Netherlands, 2016; pp. 407–434. ISBN 9780444636775. [Google Scholar]
  121. Cao, L.; Kong, Q.; Zhang, A.; Chen, X. Overexpression of SYM1 in a gpdDelta mutant of Saccharomyces cerevisiae with modified ammonium assimilation for optimization of ethanol production. J. Taiwan Inst. Chem. Eng. 2010, 41, 2–7. [Google Scholar] [CrossRef]
  122. Escudero, A.; Campo, E.; Fariña, L.; Cacho, J.; Ferreira, V. Analytical characterization of the aroma of five premium red wines. Insights into the role of odor families and the concept of fruitiness of wines. J. Agric. Food Chem. 2007, 55, 4501–4510. [Google Scholar] [CrossRef] [PubMed]
  123. Okafor, N. Microbiology and biochemistry of oil-palm wine. Adv. Appl. Microbiol. 1978, 24, 237–256. [Google Scholar]
  124. Okagbue, R.N.; Siwela, M. Yeasts and related microorganisms isolated from ripe marula fruits (Sclerocarya caffra) in Zimbabwe. S. Afr. J. Bot. 2002, 98, 551–552. [Google Scholar]
  125. Snowdon, J.A.; Cliver, D.O. Microorganisms in honey. Int. J. Food Microbiol. 1996, 31, 1–26. [Google Scholar] [CrossRef]
  126. Lasekan, O.; Buettner, A.; Christlbauer, M. Investigation of important odorants of palm wine (Elaeis guineensis). Food Chem. 2007, 105, 15–23. [Google Scholar] [CrossRef]
  127. Mwesigye, P.K.; Okurut, T.O. A Survey of the Production and Consumption of Traditional Alcoholic Beverages in Uganda. Process Biochem. 1995, 30, 497–501. [Google Scholar] [CrossRef]
  128. Shanel, K.; Matemu, A. Microbial Quality of Traditional Banana Alcoholic Beverages in Arusha, Tanzania. Food Sci. Qual. Manag. 2015, 38, 28–39. [Google Scholar]
  129. Roger, D.D.; Daoudou, B.; James, B.; Etoa, F. Artisanal production of “kuri” an honey made alcoholic beverage from Adamaoua, Cameroon. Glob. J. Sci. Res. 2014, 2, 65–70. [Google Scholar]
  130. Pereira, A.P.; Dias, T.; Andrade, J.; Ramalhosa, E.; Estevinho, L.M. Mead production: Selection and characterization assays of Saccharomyces cerevisiae strains. Food Chem. Toxicol. 2009, 47, 2057–2063. [Google Scholar] [CrossRef] [PubMed]
  131. Wyk, B. Van The potential of South African plants in the development of new food and beverage products. S. Afr. J. Bot. 2011, 77, 857–868. [Google Scholar] [CrossRef]
  132. World Health Organization. Global Status Report on Alcohol; World Health Organization: Geneva, Switzerland, 2004; pp. 4–6. [Google Scholar]
  133. Tredgold, M.H. Food Plants of Zimbabwe: With Old and New Ways of Preparation; Mambo Press: Gweru, Zimbabwe, 1986. [Google Scholar]
  134. Simatende, P. Microbial Ecology and Diversity of Swazi Traditional Fermented Foods; University of KwaZulu-Natal: Pietermaritzburg, South Africa, 2016. [Google Scholar]
  135. Sanni, A.; Lonner, C. Identification of yeasts isolated from Nigerian traditional alcoholic beverages. Food Microbiol. 1993, 10, 517–523. [Google Scholar] [CrossRef]
  136. Ramos, C.L.; Sousa, E.S.O.D.; Ribeiro, J.; Almeida, T.M.M.; Santos, C.C.A.D.A.; Abegg, M.A.; Schwan, R.F. Microbiological and chemical characteristics of tarubá, an indigenous beverage produced from solid cassava fermentation. Food Microbiol. 2015, 49, 182–188. [Google Scholar] [CrossRef] [PubMed]
  137. Kouakou, J.; Nanga, S.N.; Plagne-Ismail, C.; Pali, A.M.; Ognakossan, K.E. Cassava Production and Processing; Cameron: London, UK, 2016. [Google Scholar]
  138. Cristina, C.; Santos, A.; De Almeida, E.G.; Vinícius, G.; De Melo, P.; Schwan, R.F. Microbiological and physicochemical characterisation of caxiri, an alcoholic beverage produced by the indigenous Juruna people of Brazil. Int. J. Food Microbiol. 2012, 156, 112–121. [Google Scholar] [CrossRef]
  139. Eze, V.C.; Eleke, O.I.; Omeh, Y.S. Microbiological and nutritional qualities of burukutu sold in mammy. Am. Food J. Nutr. 2011, 1, 141–146. [Google Scholar] [CrossRef]
Figure 1. The Hyphaene petersiana (mokolwane/moxao) tree and the ripe fruits during the summer season.
Figure 1. The Hyphaene petersiana (mokolwane/moxao) tree and the ripe fruits during the summer season.
Beverages 04 00036 g001
Figure 2. The marula tree (Sclerocarya birrea sub-species caffra) and the marula fruits.
Figure 2. The marula tree (Sclerocarya birrea sub-species caffra) and the marula fruits.
Beverages 04 00036 g002
Figure 3. Grewia flava tree (mogwana) and khadi.
Figure 3. Grewia flava tree (mogwana) and khadi.
Beverages 04 00036 g003
Figure 4. Tej—a cloudy unrefined honey wine.
Figure 4. Tej—a cloudy unrefined honey wine.
Beverages 04 00036 g004
Figure 5. Floor mounted fermentation vessels in Zimbabwe.
Figure 5. Floor mounted fermentation vessels in Zimbabwe.
Beverages 04 00036 g005
Figure 6. Basic yeast metabolism. Secondary metabolites are shown in (pink) and metabolism intermediates (lime, orange, purple and blue) are also shown.
Figure 6. Basic yeast metabolism. Secondary metabolites are shown in (pink) and metabolism intermediates (lime, orange, purple and blue) are also shown.
Beverages 04 00036 g006
Table 1. Characteristics of substrates commonly used in the production of non-cereal-based alcoholic beverages.
Table 1. Characteristics of substrates commonly used in the production of non-cereal-based alcoholic beverages.
SubstrateTotal Sugar Content *SugarspHNitrogen SourcesReferences
Honey79.5%Glucose (38.19%), fructose (31.28%), sucrose (1.31%), maltose, isomaltose, trehalose, trisaccharideserlose, raffinose, melezitose, tetrasaccharides and pentasaccharides3.4–6.1Proline, glutamic acid, aspartic acid, glutamine, histidine, glycine, arginine, tryptophan, and cysteine[31,32,33]
Palm sap
(Palmae family)
10–18%Sucrose (36%), glucose (33%), fructose, cellobiose, dextran, maltose, xylose, hamnose, arabinose and galacturonic acid7–7.4Valine, threonine, lysine and phenylalanine[34,35,36]
Masau
(Ziziphus mauritiana)
13.7%Glucose (6.7%) and fructose (6.8%)5.6–6.6 [37]
Pineapple
(Ananas comosus)
7.98%Sucrose, fructose and glucose3.5 [38,39]
Plantain
(Musa paradisiaca)
20–27%Glucose, fructose and sucrose4.26Arginine, aspartic acid, glutamic acid and methionine[40,41]
Banana
(Musa acuminata)
14.20–20.18%Glucose, fructose and sucrose4.78Aspartic acid, histidine, leucine and valine[42,43]
Cassava
(Manihot esculenta)
4.04–18.47%Sucrose (1.98–15.40%), maltose, fructose, and glucose6.2–6.9Valine, leucine, isoleucine, phenylalanine, tryptophan, methionine, threonine, histidine and lysine [44,45,46,47,48]
Marula juice
(Sclerocarya birrea)
8.2%Sucrose (5.9%), fructose and glucose4.10Methionine, cysteine, leucine, phenylalanine, lysine, and threonine[49,50,51]
* The percentages of the fermentable sugars are per 100 g.
Table 2. Microbial diversity of non-cereal-based alcoholic beverages.
Table 2. Microbial diversity of non-cereal-based alcoholic beverages.
Alcoholic BeverageRaw Materials/SubstrateSensory PropertiesNatureFermentative MicrobesSecondary MetabolitesCountryReferences
TejHoney SweetEffervescent, yellow andcloudySaccharomyces cerevisiae, Kluyveromyces bulgaricus, Debaromyces phaffi, K. veronae, Zygosaccharomyces rouxii, Hanensula subpeliculosa, S. norbensis, K. vanudenii, Endomycopsis burtonii, Lactobacillus spp., Streptococcus spp., Leuconostoc spp., and Pediococcus spp.Ethanol, methanol, esters and organic acidsEthiopia[11,27,52]
UrwagwaBanana pulpPungent, fruity, herbaceous and astringent/acidic tasteCloudySaccharomyces cerevisiaeEthanol, 1-propanol, 2-hexanol, acetic acid, 5-hexanoic acid, benzoic acid, propanoic acid, formic acid, ethyl acetate, butanoic acid, ethyl ester, 1-Butanol 3-methyl-ethyl ester and hexanoic acid ethyl esterRwanda, Burundi, Uganda, Tanzania and Kenya[53]
Palm winePalm tree sapSour, heavy and astringent tasteWhitish, milk-white opalescent liquidStaphylococcus spp., Micrococcus spp., Serratia spp., Bacillus spp., Streptococcus spp., Saccharomyces cerevisae, S. pombe, Candida tropicalis, C. krusei, Candida spp., Pichia spp., S. chevalieri, Zymomonas mobilis, Hanseniaspora guilliermondii, H. uvarum, Lactobacillus plantarum, Leuconostoc mesenteroides, L. nagelii, L. sucicola, Acetobacter pasteurianus, A. indonesiensis and Gluconobacter spp.Lactic acid, citric acid, tartaric acids, ethanol and acetic acid, esters, carbonyls, higher alcohols, phenols, sulphur compounds, terpenes, hydrocarbons, acetals, nitrogen compounds and lactoneNigeria, Bukina Faso and Cameroon[34,35,54,55]
KhadiGrewia flava, Grewia occidentalis, Grewia flavascens, Kedrostis hirtella and Khadia acutipetalaSweet Reddish colorNo literature on fermentative microbesEthanol, 2-methyl-1-propanol, 2/3-methyl-1-butanol, ethyl lactate and ethyl acetateBotswana[56]
MukumbiMarula fruitsThick creamy liquorYellowish-brownSaccharomyces cerevisiae, Pichia anomala, P. guilliermondii, Candida tropicalis, and C. intermediaEthanolNamibia, Botswana, Swaziland, Zimbabwe and Zambia[9,57]
MuchemaPalm tree sapSour Brownish to colorlessNo literature on the microbial diversityEthanol Botswana and Namibia[57]
Pineapple winePineapplesFruity, sweetand cream/fatty notesGolden yellowish Hanseniaspora guilliermondii, Pichia anomala, Meyerozyma guilliermondii, H. uvarum, Wickerhamomyces anomalus and H. opuntiaeAcetic acid, ethyl acetate, isobutanol, 3-methyl-1-butanol, 2-methyl-1-butanol, ethyl 2-methylpropanoate, 3-methyl-1-butyl acetate, ethyl hexanoate, methyl benzoate, 2-phenylethanol, methyl 2-methylheptanoate, ethyl benzoate, diethyl succinate, ethyl octanoate, ethyl phenylacetate, 2-phenylethyl acetate, ethyl 9-decenoate and ethyl decanoateAngola[38,58,59,60]
KachasuMasau fruits (Ziziphus mauritiana), Adansonia digitate and Tamarindus indicaSour, acidicClear distillateSaccharomyces cerevisiae, Issatchenkia orientalis, Pichia fabianii, Aureobasidium pullulans, Lactobacillus agilis, L. minor, L. confusus, L. fructosus, L. bifermentans, L. divergens, L. fermentum, L. hilgardii and Streptococcus spp.Isoamyl alcohol, isobutanol, methanol, acetaldehyde, acetone, ethyl acetate, and furfuralsZimbabwe[5,9]
Table 3. Contribution of secondary fermentation metabolites to flavor and aroma of alcoholic beverages.
Table 3. Contribution of secondary fermentation metabolites to flavor and aroma of alcoholic beverages.
Metabolite ClassExamples of CompoundsContribution to Flavor and AromaReferences
Organic acidsSuccinic acid, acetic acid, lactic acid, citric acidThese compounds contribute to the astringency of fermented beverages. The presence of some acids, notably lactic acid, can indicate undesirable bacterial spoilage.[72,73,117]
Higher alcoholsIsoamyl alcohol, phenylethanol, isopropanolThese impart desirable flavor and aromas to fermented beverages but only within certain limits[114]
Carbonyl compoundsAcetaldehyde, aldehydes, ketonesAbove its flavor threshold in beverages, this compound can impart a “grassy” or “green apple” (related to acetaldehyde) flavor but this can be removed by secondary yeast fermentation during conditioning.[72,76]
Vicinal diketonesDiacetyl, pentane-2,3-dioneDiacetyl in most beverages is undesirable, imparting a rancid-butter or “butterscotch” flavor.[118,119,120]
PolyolsGlycerol This compound is produced during normal yeast metabolism or when yeasts are confronted with osmotic stress. Glycerol contributes desirable viscosity to fermented beverages, notably wines, the body of the wine per se.[21,76,121]
Sulfur compoundsHydrogen sulphide, dimethyl sulphide, sulphur dioxide, ThiolsThese are important beverage flavor and aroma compounds. For example, in beer, dimethyl sulphide (DMS) in low concentration is a desirable attribute of lagers, but higher concentration imparts off-flavors.[67,122]
EstersEthyl acetateAssociated with a fruity aroma and floral flavors and aromas to fermented beverages.[79,80]

© 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
Beverages EISSN 2306-5710 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top