Macroscopic Dynamic Modeling of Sequential Batch Cultures of Hybridoma Cells: An Experimental Validation
AbstractHybridoma cells are commonly grown for the production of monoclonal antibodies (MAb). For monitoring and control purposes of the bioreactors, dynamic models of the cultures are required. However these models are difficult to infer from the usually limited amount of available experimental data and do not focus on target protein production optimization. This paper explores an experimental case study where hybridoma cells are grown in a sequential batch reactor. The simplest macroscopic reaction scheme translating the data is first derived using a maximum likelihood principal component analysis. Subsequently, nonlinear least-squares estimation is used to determine the kinetic laws. The resulting dynamic model reproduces quite satisfactorily the experimental data, as evidenced in direct and cross-validation tests. Furthermore, model predictions can also be used to predict optimal medium renewal time and composition. View Full-Text
Scifeed alert for new publicationsNever miss any articles matching your research from any publisher
- Get alerts for new papers matching your research
- Find out the new papers from selected authors
- Updated daily for 49'000+ journals and 6000+ publishers
- Define your Scifeed now
Dewasme, L.; Côte, F.; Filee, P.; Hantson, A.-L.; Vande Wouwer, A. Macroscopic Dynamic Modeling of Sequential Batch Cultures of Hybridoma Cells: An Experimental Validation. Bioengineering 2017, 4, 17.
Dewasme L, Côte F, Filee P, Hantson A-L, Vande Wouwer A. Macroscopic Dynamic Modeling of Sequential Batch Cultures of Hybridoma Cells: An Experimental Validation. Bioengineering. 2017; 4(1):17.Chicago/Turabian Style
Dewasme, Laurent; Côte, François; Filee, Patrice; Hantson, Anne-Lise; Vande Wouwer, Alain. 2017. "Macroscopic Dynamic Modeling of Sequential Batch Cultures of Hybridoma Cells: An Experimental Validation." Bioengineering 4, no. 1: 17.
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.