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Bioengineering 2014, 1(3), 134-153; doi:10.3390/bioengineering1030134

Fabrication of Bioactive Surfaces by Functionalization of Electroactive and Surface-Active Block Copolymers

1
Center for Functional Nanoscale Materials and Department of Chemistry, Clark Atlanta University, Atlanta, GA 30314, USA
2
Center for Cancer Research and Therapeutic Development, Clark Atlanta University, Atlanta, GA 30314, USA
3
Department of Physics, Clark Atlanta University, Atlanta, GA 30314, USA
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Received: 29 May 2014 / Revised: 11 August 2014 / Accepted: 14 August 2014 / Published: 20 August 2014
(This article belongs to the Special Issue Biofabrication)
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Abstract

Biofunctional block copolymers are becoming increasingly attractive materials as active components in biosensors and other nanoscale electronic devices. We have described two different classes of block copolymers with biofuctional properties. Biofunctionality for block copolymers is achieved through functionalization with appropriate biospecific ligands. We have synthesized block copolymers of electroactive poly(3-decylthiophene) and 2-hydroxyethyl methacrylate by atom transfer radical polymerization. The block copolymers were functionalized with the dinitrophenyl (DNP) groups, which are capable of binding to Immunoglobulin E (IgE) on cell surfaces. The block copolymers were shown to be redox active. Additionally, the triblock copolymer of α, ω-bi-biotin (poly(ethylene oxide)-b-poly (styrene)-b-poly(ethylene oxide)) was also synthesized to study their capacity to bind fluorescently tagged avidin. The surface-active property of the poly(ethylene oxide) block improved the availability of the biotin functional groups on the polymer surfaces. Fluorescence microscopy observations confirm the specific binding of biotin with avidin. View Full-Text
Keywords: block copolymers; biosensor; ionic polymerization; atom transfer radical polymerization; fluorescence microscope block copolymers; biosensor; ionic polymerization; atom transfer radical polymerization; fluorescence microscope
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This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Olubi, O.; London, L.; Sannigrahi, B.; Nagappan, P.; Williams, M.; Khan, I.M. Fabrication of Bioactive Surfaces by Functionalization of Electroactive and Surface-Active Block Copolymers. Bioengineering 2014, 1, 134-153.

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