Table of Contents
Toxics, Volume 5, Issue 4 (December 2017)
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Cover Story (view full-size image) Ethanol exposure during embryogenesis produces developmental defects including congenital heart [...] Read more. Ethanol exposure during embryogenesis produces developmental defects including congenital heart defects that resemble defects seen in humans. To visualize early- and late-added cardiac precursors, a Tg(myl7:nlsKikGR) green-to-red photoconvertible protein KikGR was used, which fluoresces green but is photoconverted to red by a brief exposure to UV light. Control (left image) and ethanol exposed (right image) embryos were photoconverted at 28 hpf and imaged (three-dimensional reconstruction of confocal images) at 48 hpf, showing both red-and-green cardiomyocytes (primarily first heart field origin) and the green-only cardiomyocytes (second heart filed, cardiac neural crest origin). View the paper here.