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Toxics 2016, 4(4), 26; doi:10.3390/toxics4040026

Fish Reproduction Is Disrupted upon Lifelong Exposure to Environmental PAHs Fractions Revealing Different Modes of Action

1
Ifremer, Ecotoxicology Laboratory, Place Gaby Coll, F-17137 L’Houmeau, France
2
INRA UMR703, APEX, Oniris, F-44307 Nantes, France
3
Oniris, École Nationale vétérinaire, Agro-Alimentaire et de L’alimentation Nantes-Atlantique, LUNAM Université, F-44307 Nantes, France
4
Department of Science and Technology, University of Bordeaux 1, EPOC, UMR CNRS 5805, F-33405 Talence, France
5
Ifremer, Fisheries Laboratory, Place Gaby Coll, F-17137 L’Houmeau, France
6
INRA LPGP, Campus de Beaulieu, F-35042 Rennes, France
7
Ifremer, Laboratoire Adaptation et Adaptabilité des Animaux et des Systèmes, UMR MARBEC, Route de Maguelone, F-34250 Palavas les Flots, France
*
Author to whom correspondence should be addressed.
Academic Editor: Robert Tanguay
Received: 18 July 2016 / Revised: 17 October 2016 / Accepted: 19 October 2016 / Published: 28 October 2016
(This article belongs to the Special Issue Zebrafish as a Model for Assessing Chemical Toxicity)
View Full-Text   |   Download PDF [2218 KB, uploaded 28 October 2016]   |  

Abstract

Polycyclic aromatic hydrocarbons (PAHs) constitute a large family of organic pollutants emitted in the environment as complex mixtures, the compositions of which depend on origin. Among a wide range of physiological defects, PAHs are suspected to be involved in disruption of reproduction. In an aquatic environment, the trophic route is an important source of chronic exposure to PAHs. Here, we performed trophic exposure of zebrafish to three fractions of different origin, one pyrolytic and two petrogenic. Produced diets contained PAHs at environmental concentrations. Reproductive traits were analyzed at individual, tissue and molecular levels. Reproductive success and cumulative eggs number were disrupted after exposure to all three fractions, albeit to various extents depending on the fraction and concentrations. Histological analyses revealed ovary maturation defects after exposure to all three fractions as well as degeneration after exposure to a pyrolytic fraction. In testis, hypoplasia was observed after exposure to petrogenic fractions. Genes expression analysis in gonads has allowed us to establish common pathways such as endocrine disruption or differentiation/maturation defects. Taken altogether, these results indicate that PAHs can indeed disrupt fish reproduction and that different fractions trigger different pathways resulting in different effects. View Full-Text
Keywords: polycyclic aromatic hydrocarbons; zebrafish; spawning success; fertilization; gonad differentiation; molecular mechanisms polycyclic aromatic hydrocarbons; zebrafish; spawning success; fertilization; gonad differentiation; molecular mechanisms
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Vignet, C.; Larcher, T.; Davail, B.; Joassard, L.; Le Menach, K.; Guionnet, T.; Lyphout, L.; Ledevin, M.; Goubeau, M.; Budzinski, H.; Bégout, M.-L.; Cousin, X. Fish Reproduction Is Disrupted upon Lifelong Exposure to Environmental PAHs Fractions Revealing Different Modes of Action. Toxics 2016, 4, 26.

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