Application of Gut Cell Models for Toxicological and Bioactivity Studies of Functional and Novel Foods
Abstract
:1. Introduction
2. Current Approaches in In Vitro Toxicology
3. Cell Cultures
Cell Line/Model | Origin | Type | Status | Species | Supplier |
---|---|---|---|---|---|
HIEC-6 | Small intestine | Epithelia | Normal | Human | University ofSherbrooke a |
H4 | Small intestinal foetal tissue | Epithelia | Normal | Human | MassachusettsGeneral Hospital b |
H4-1 | Small intestinal foetal tissue | Epithelia | Normal | Human | BioNutriTech c |
PSI-1 | Mature small intestine | Epithelia | Normal | Pig | BioNutriTech c |
CLAB | Enterocytes | Epithelia | Normal | Pig | BioNutriTech c |
Pom 2 | Blood | Monocytes | Normal | Pig | BioNutriTech c |
TLT | Blood | Monocytes | Normal | Human | BioNutriTech c |
Gut 3D model | Functional | Normal | Human | BioNutriTech c | |
Gut 3D model | Functional | Normal | Pig | BioNutriTech c |
4. Available In Vitro Cell Models for Risk Assessment and Toxicology Studies of Functional Foods
- Which cell line has the most relevant phenotype for a designed study?
- Which other organ systems have an influence in vivo on our employed system and how can we integrate them in our study?
- Is the model validated and if not, which validated model will be used as comparative control?
Study | Parameter/Implications | References |
---|---|---|
Trans-epithelial electrical resistance (TEER) | Cell differentiation, connectedness, polarization, intestinal integrity | [8,18,32,35] |
Bioaccessibility, absorption and biotransformation | Transition from the apical to the basal compartment and vice versa, cellular absorption, transformation | [8,18,24,25,36,37] |
Host-microbe interactions | Attachment, communication, migration, influence on epithelial function, simulation of normal gut microflora, their influence on biotransformation as well as absorption of bioactives | [26,31,37,38,39] |
Communication | Cell-cell, cell cross-talk, expression of cytokines, chemokines, nuclear factors, connexins... | [1,8,32,38,40,41] |
Immunomodulation | Expression of cytokines and nuclear factors in separated apical and basal compartments, immunoprofiling, pro- and anti-inflammatory orientation | [8,32,37,38,40] |
Custom bioassays | Combination of different strategies, combination of different cell lines, HTS integration | [13,30] |
5. Conclusions
Acknowledgments
Conflicts of Interest
References
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Trapecar, M.; Cencic, A. Application of Gut Cell Models for Toxicological and Bioactivity Studies of Functional and Novel Foods. Foods 2012, 1, 40-51. https://doi.org/10.3390/foods1010040
Trapecar M, Cencic A. Application of Gut Cell Models for Toxicological and Bioactivity Studies of Functional and Novel Foods. Foods. 2012; 1(1):40-51. https://doi.org/10.3390/foods1010040
Chicago/Turabian StyleTrapecar, Martin, and Avrelija Cencic. 2012. "Application of Gut Cell Models for Toxicological and Bioactivity Studies of Functional and Novel Foods" Foods 1, no. 1: 40-51. https://doi.org/10.3390/foods1010040