Next Article in Journal
Integrated Computational Model of Intracellular Signaling and microRNA Regulation Predicts the Network Balances and Timing Constraints Critical to the Hepatic Stellate Cell Activation Process
Next Article in Special Issue
Special Issue: Design of Bioreactor Systems for Tissue Engineering
Previous Article in Journal
Mathematical Modeling of Microbial Community Dynamics: A Methodological Review
Previous Article in Special Issue
A Novel Through-Thickness Perfusion Bioreactor for the Generation of Scaffold-Free Tissue Engineered Cartilage
Article Menu

Export Article

Open AccessArticle
Processes 2014, 2(4), 753-772; doi:10.3390/pr2040753

Experimental Characterisation of Fluid Mechanics in a Spinner Flask Bioreactor

1
Division of Biological Engineering, Monash University, Melbourne, VIC 3800, Australia
2
Department of Mechanical and Aerospace Engineering, Monash University, Melbourne, VIC 3800, Australia
*
Author to whom correspondence should be addressed.
Received: 28 July 2014 / Revised: 1 September 2014 / Accepted: 30 September 2014 / Published: 17 October 2014
(This article belongs to the Special Issue Design of Bioreactor Systems for Tissue Engineering)
View Full-Text   |   Download PDF [10437 KB, uploaded 17 October 2014]   |  

Abstract

The spinner flask bioreactor has been widely used in in vitro cell culturing processes due to its superiority in providing a homogeneous culture environment compared to traditional culturing methods. However, there is limited understanding of the flow fields in these bioreactors, and optimum culture conditions are yet to be determined. This article presents the experimental characterization of the flow field within a spinner flask at varying speeds (10 RPM to 80 RPM) and impeller positions. An optical, non-invasive measurement technique, Particle Image Velocimetry (PIV), was employed to illustrate the fluid flow and calculate the stresses and vorticity associated with the flow within the flask. The largest recirculation structure was observed in the meridional plane at the highest impeller position while the highest shear stress region was observed at the base of the spinner flask. The study provides an overview of the fluid structure within the spinner flask in the meridional and azimuthal planes. Furthermore, the results presented in this study give an accurate quantification of the range of stresses for the given impeller speeds. These results provide estimates of the biomechanical properties within the type of spinner flask used in many published cell studies. View Full-Text
Keywords: fluid dynamics; spinner flask; cell culture; shear; hydrodynamic force; velocimetry; particle image velocimetry fluid dynamics; spinner flask; cell culture; shear; hydrodynamic force; velocimetry; particle image velocimetry
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Ismadi, M.-Z.; Hourigan, K.; Fouras, A. Experimental Characterisation of Fluid Mechanics in a Spinner Flask Bioreactor. Processes 2014, 2, 753-772.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Processes EISSN 2227-9717 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top