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From the first issue of 2016, Chromatography has changed its name to Separations.

Open AccessArticle
Chromatography 2015, 2(3), 502-514; doi:10.3390/chromatography2030502

Evaluation of 5 μm Superficially Porous Particles for Capillary and Microfluidic LC Columns

1
Department of Chemistry, University of Michigan, Ann Arbor, MI 48109, USA
2
Department of Pharmacology, University of Michigan, Ann Arbor, MI 48109, USA
*
Author to whom correspondence should be addressed.
Academic Editor: Christopher Palmer
Received: 26 May 2015 / Revised: 10 July 2015 / Accepted: 24 July 2015 / Published: 4 August 2015
(This article belongs to the Special Issue Microscale Separation and Analysis)
View Full-Text   |   Download PDF [1286 KB, uploaded 4 August 2015]   |  

Abstract

Large-size (4–5 µm) superficially porous particles yield lower plate heights (e.g., the minimal reduced plate height or hmin ≈ 1.5) than fully porous particles of a similar size when packed into large-bore columns. This property allows for better chromatographic performance without the higher pressures required for smaller particles. This study explores the use of such particles in microfluidic LC columns where materials and fitting pressure limits can constrain the size of particle used. The theoretically predicted performance improvements compared to fully porous particles were not demonstrated in capillary columns (with hmin ≈ 2 for both particle types), in agreement with previous studies that examined smaller superficially porous particles. Microfluidic columns were then compared to capillary columns. Capillary columns significantly outperformed microfluidic columns due to imperfections imposed by microfluidic channel asymmetry and world-to-chip connection at the optimal flow rate; however, superficially porous particles packed in microfluidic LC columns had flatter plate height versus flow rate curves indicating potential for better performance at high reduced velocities. View Full-Text
Keywords: liquid chromatography; superficially porous particles; core-shell; microfluidics; chip-LC; capillary LC liquid chromatography; superficially porous particles; core-shell; microfluidics; chip-LC; capillary LC
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Grinias, J.P.; Kennedy, R.T. Evaluation of 5 μm Superficially Porous Particles for Capillary and Microfluidic LC Columns. Chromatography 2015, 2, 502-514.

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