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From the first issue of 2016, Chromatography has changed its name to Separations.

Open AccessArticle
Chromatography 2015, 2(1), 125-140; doi:10.3390/chromatography2010125

Analysis of Bioactive Components of Oilseed Cakes by High-Performance Thin-Layer Chromatography-(Bio)assay Combined with Mass Spectrometry

1
Chair of Food Science, Institute of Nutritional Science, Justus Liebig University Giessen, Heinrich-Buff-Ring 26-32, 35392 Giessen, Germany
2
On leave from Department of Food Science, University of Otago, PO Box 56, Dunedin 9054, New Zealand
*
Author to whom correspondence should be addressed.
Academic Editor: Mark Devlin Maloney
Received: 31 December 2014 / Accepted: 9 March 2015 / Published: 17 March 2015
(This article belongs to the Special Issue New Trends in Thin-Layer Chromatography)
View Full-Text   |   Download PDF [2435 KB, uploaded 17 March 2015]   |  

Abstract

Hemp, flax and canola seed cakes are byproducts of the plant oil extraction industry that have not received much attention in terms of their potential use for human food instead of animal feed. Thus, the bioactivity profiling of these oilseed cakes is of interest. For their effect-directed analysis, planar chromatography was combined with several (bio)assays, namely 2,2-diphenyl-1-picrylhydrazyl scavenging, acetylcholine esterase inhibition, planar yeast estrogen screen, antimicrobial Bacillus subtilis and Aliivibrio fischeri assays. The streamlined high-performance thin-layer chromatography (HPTLC)-bioassay method allowed the discovery of previously unknown bioactive compounds present in these oilseed cake extracts. In contrast to target analysis, the direct link to the effective compounds allowed comprehensive information with regard to selected effects. HPTLC-electrospray ionization-mass spectrometry via the elution-head based TLC-MS Interface was used for a first characterization of the unknown effective compounds. The demonstrated bioactivity profiling on the feed/food intake side may guide the isolation of active compounds for production of functional food or for justified motivation of functional feed/food supplements. View Full-Text
Keywords: oilseeds; hemp; flax; canola; polyphenols; planar chromatography; effect-directed analysis; HPTLC-MS; bioassays oilseeds; hemp; flax; canola; polyphenols; planar chromatography; effect-directed analysis; HPTLC-MS; bioassays
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Teh, S.-S.; Morlock, G.E. Analysis of Bioactive Components of Oilseed Cakes by High-Performance Thin-Layer Chromatography-(Bio)assay Combined with Mass Spectrometry. Chromatography 2015, 2, 125-140.

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