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Chromatography 2013, 1(1), 1-8; doi:10.3390/chromatography1010001

Article

Oroxylum indicum Seeds—Analysis of Flavonoids by Micellar Electrokinetic Chromatography

Anja Krüger and Markus Ganzera*

Institute of Pharmacy, Pharmacognosy, University of Innsbruck, Innrain 80-82, 6020 Innsbruck, Austria

* Author to whom correspondence should be addressed.

Received: 5 January 2013; in revised form: 16 February 2013 / Accepted: 19 February 2013 / Published: 25 February 2013

Download PDF Full-Text [315 KB, Updated Version, uploaded 28 March 2013 17:55 CET]

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Abstract: Flavonoids are bioactive constituents in Oroxylum indicum seeds, an Asian traditional remedy used for the treatment of respiratory infections. In this study the first capillary electrophoretic method for their determination is presented. By using a 25 mM borax buffer at pH 9.2 containing 10 mM SDS as detergent, the determination of seven flavonoids was feasible in only 13 min. Method validation confirmed that the assay is in accordance with ICH requirements in respect to linearity, selectivity, sensitivity, accuracy and precision. Quantitative results revealed that baicalein-7-O-gentiobioside is the most abundant flavonoid in the drug (1.19 to 5.33%), followed by other baicalein derivatives (7-O-glucoside, 7-O-glucuronide). These observations were in good qualitative and quantitative agreement with LC-MS results.

Keywords: Oroxylum indicum; seeds; capillary electrophoresis; MEKC; flavonoids

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