Next Article in Journal
Plasticizer Effects in the PVC Membrane of the Dibasic Phosphate Selective Electrode
Previous Article in Journal / Special Issue
New Nanomaterials and Luminescent Optical Sensors for Detection of Hydrogen Peroxide
Article Menu

Export Article

Open AccessArticle
Chemosensors 2015, 3(4), 274-283; doi:10.3390/chemosensors3040274

Electrochemical Sensors for the Estimation of the Inhibitory Effect of Phenylcarbamates to Cholinesterase

1
Department of Biological and Biochemical Sciences, Faculty of Chemical Technology, University of Pardubice, Studentská 573, 532 10 Pardubice, Czech Republic
2
Department of Analytical Chemistry, Faculty of Chemical Technology, University of Pardubice, Studentská 573, 532 10 Pardubice, Czech Republic
3
Institute of Organic Chemistry and Technology, Faculty of Chemical Technology, University of Pardubice, Studentská 573, 532 10 Pardubice, Czech Republic
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Academic Editor: Igor Medintz
Received: 9 October 2015 / Revised: 25 November 2015 / Accepted: 2 December 2015 / Published: 10 December 2015
(This article belongs to the Special Issue Feature Paper)
View Full-Text   |   Download PDF [460 KB, uploaded 10 December 2015]   |  

Abstract

The inhibitory effect of nine phenylcarbamates with various substituents was studied. For this purpose, electrochemical sensors were applied under two different conditions: if an enzyme was present in the solution or if the enzyme was immobilized onto the electrode surface. In both cases, 3-[(ethoxycarbonyl)amino]phenyl (4-chloro-phenyl)carbamate was found as the most effective inhibitor for butyrylcholinesterase. The best inhibitors for acetylcholinesterase were determined as well, depending on the used method. Thus, 3‑[(butoxycarbonyl)-amino]phenyl phenylcarbamate with the enzyme present in the solution and 3-[(ethoxycarbonyl)amino]phenyl (3-methylphenyl)carbamate when the enzyme was immobilized onto the electrode surface were evaluated as the most effective inhibitors. Michaelis constants as well as maximum reaction rates were calculated and assessed. View Full-Text
Keywords: biosensors; immobilization of cholinesterases; phenylcarbamates; inhibition; IC50; kinetic parameters biosensors; immobilization of cholinesterases; phenylcarbamates; inhibition; IC50; kinetic parameters
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Vorčáková, K.; Štěpánková, Š.; Sedlák, M.; Vytřas, K. Electrochemical Sensors for the Estimation of the Inhibitory Effect of Phenylcarbamates to Cholinesterase. Chemosensors 2015, 3, 274-283.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Chemosensors EISSN 2227-9040 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top