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Chemosensors 2015, 3(2), 118-128; doi:10.3390/chemosensors3020118

Development of an Electrochemical Sensor for NADH Determination Based on a Caffeic Acid Redox Mediator Supported on Carbon Black

1
Department of Chemical and Geological Sciences, University of Modena and Reggio Emilia, via G. Campi, 41125 Modena, Italy
2
Department of Chemical Sciences and Technologies, University of Tor Vergata, via della Ricerca Scientifica, 00133 Roma, Italy
3
Institute of Organic Synthesis and Photoreactivity (ISOF)—National Research Council of Italy (CNR), via Gobetti 101, 40129 Bologna, Italy
*
Author to whom correspondence should be addressed.
Academic Editors: Paolo Ugo and Michael Ongaro
Received: 27 February 2015 / Revised: 27 March 2015 / Accepted: 1 April 2015 / Published: 13 April 2015
(This article belongs to the Special Issue Electrochemical Sensors for Environmental and Food Analysis)
View Full-Text   |   Download PDF [495 KB, uploaded 13 April 2015]   |  

Abstract

Screen-printed electrode (SPE) modified with carbon black nanoparticles (CB) has been tested as a new platform for the stable deposition of caffeic acid (CFA) on the electrode surface. The electrochemical performance from varying the amount of CFA/CB composite has been tested with respect to NADH determination. The electrocatalytic activity of CFA/CB has also been compared with that of SPEs modified by a single component of the coating, i.e., either CFA or CB. Finally, glycerol dehydrogenase, a typical NADH-dependent enzyme, was deposited on the CFA/CB coating in order to test the applicability of the sensor in glycerol determination. View Full-Text
Keywords: screen-printed electrodes; amperometric sensor; enzymatic biosensors; NADH; caffeic acid; carbon black; glycerol dehydrogenase; glycerol determination screen-printed electrodes; amperometric sensor; enzymatic biosensors; NADH; caffeic acid; carbon black; glycerol dehydrogenase; glycerol determination
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Zanardi, C.; Ferrari, E.; Pigani, L.; Arduini, F.; Seeber, R. Development of an Electrochemical Sensor for NADH Determination Based on a Caffeic Acid Redox Mediator Supported on Carbon Black. Chemosensors 2015, 3, 118-128.

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