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Proteomes 2018, 6(1), 9; doi:10.3390/proteomes6010009

Proteome Profiling of Diabetic Mellitus Patient Urine for Discovery of Biomarkers by Comprehensive MS-Based Proteomics

1
Biofluid Biomarker Center, Niigata University, Niigata 950-2181, Japan
2
Department of Pediatrics, National Hospital Organization Niigata Hospital, Kashiwazaki 945-8585, Japan
3
Department of Nephrology, Shinrakuen Hospital, Niigata 950-2087, Japan
*
Author to whom correspondence should be addressed.
Received: 27 December 2017 / Revised: 19 January 2018 / Accepted: 2 February 2018 / Published: 6 February 2018
(This article belongs to the Special Issue Proteomic Biomarkers in Human Diseases)
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Abstract

Diabetic mellitus (DM) is a disease that affects glucose homeostasis and causes complications, such as diabetic nephropathy (DN). For early diagnosis of DN, microalbuminuria is currently one of the most frequently used biomarkers. However, more early diagnostic biomarkers are desired in addition to microalbuminuria. In this study, we performed comprehensive proteomics analysis of urine proteomes of diabetic mellitus patients without microalbuminuria and healthy volunteers to compare the protein profiles by mass spectrometry. With high confidence criteria, 942 proteins in healthy volunteer urine and 645 proteins in the DM patient urine were identified with label-free semi-quantitation, respectively. Gene ontology and pathway analysis were performed with the proteins, which were up- or down-regulated in the DM patient urine to elucidate significant changes in pathways. The discovery of a useful biomarker for early DN discovery is expected. View Full-Text
Keywords: urine; biomarker; diabetic mellitus urine; biomarker; diabetic mellitus
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Hirao, Y.; Saito, S.; Fujinaka, H.; Miyazaki, S.; Xu, B.; Quadery, A.F.; Elguoshy, A.; Yamamoto, K.; Yamamoto, T. Proteome Profiling of Diabetic Mellitus Patient Urine for Discovery of Biomarkers by Comprehensive MS-Based Proteomics. Proteomes 2018, 6, 9.

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