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Proteomes 2018, 6(1), 8; doi:10.3390/proteomes6010008

In-Depth Proteomic Characterization of Classical and Non-Classical Monocyte Subsets

1
Proteomics, Genomics and Bioinformatics Unit, Center for Applied Medical Research, University of Navarra, Pamplona 31008, Spain
2
Proteomics Unit; Central Service for Experimental Research (SCSIE), University of Valencia. Dr Moliner 50, 46100 Burjassot, Spain
3
Spanish National Cancer Research Centre (CNIO), Melchor Férnandez Almagro, 3, 28029 Madrid. Spain
4
Proteomics Core Facility-SGIKER, University of the Basque Country, UPV/EHU, 48940 Leioa, Spain
5
Department of Biochemistry and Molecular Biology, University of the Basque Country, UPV/EHU, 48940 Leioa, Spain
6
Proteomics Unit, Centro Nacional de Biotecnología-CSIC, Darwin 3, 28049 Madrid, Spain
7
Department of Medicine and General Cytometry Service-Nucleus, Cancer Research Centre (IBMCC/CSIC/USAL/IBSAL), 37007 Salamanca, Spain
8
Proteomics Unit. Cancer Research Centre (IBMCC/CSIC/USAL/IBSAL), 37007 Salamanca, Spain
9
Cancer Research Center. University of Salamanca-CSIC, IBSAL, 37007 Salamanca, Spain
10
Spanish National DNA Bank Carlos III, University of Salamanca, 37007 Salamanca, Spain
11
Department of Biochemistry and Molecular Biology, University of Valencia. Dr Moliner 50, 46100 Burjassot, Spain
12
Biotechnology and Biomedicine Interdisciplinary Research Unit (ERI BIOTECMED), University of Valencia. Dr Moliner 50, 46100 Burjassot, Spain
*
Author to whom correspondence should be addressed.
Received: 7 December 2017 / Revised: 24 January 2018 / Accepted: 1 February 2018 / Published: 5 February 2018
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Abstract

Monocytes are bone marrow-derived leukocytes that are part of the innate immune system. Monocytes are divided into three subsets: classical, intermediate and non-classical, which can be differentiated by their expression of some surface antigens, mainly CD14 and CD16. These cells are key players in the inflammation process underlying the mechanism of many diseases. Thus, the molecular characterization of these cells may provide very useful information for understanding their biology in health and disease. We performed a multicentric proteomic study with pure classical and non-classical populations derived from 12 healthy donors. The robust workflow used provided reproducible results among the five participating laboratories. Over 5000 proteins were identified, and about half of them were quantified using a spectral counting approach. The results represent the protein abundance catalogue of pure classical and enriched non-classical blood peripheral monocytes, and could serve as a reference dataset of the healthy population. The functional analysis of the differences between cell subsets supports the consensus roles assigned to human monocytes. View Full-Text
Keywords: monocytes; protein profiling; quantitative proteomics monocytes; protein profiling; quantitative proteomics
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Segura, V.; Valero, M.L.; Cantero, L.; Muñoz, J.; Zarzuela, E.; García, F.; Aloria, K.; Beaskoetxea, J.; Arizmendi, J.M.; Navajas, R.; Paradela, A.; Díez, P.; Dégano, R.M.; Fuentes, M.; Orfao, A.; García Montero, A.; Garin-Muga, A.; Corrales, F.J.; Sánchez del Pino, M.M. In-Depth Proteomic Characterization of Classical and Non-Classical Monocyte Subsets. Proteomes 2018, 6, 8.

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