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Proteomes 2016, 4(3), 20; doi:10.3390/proteomes4030020

Extracellular Matrix Proteome and Phosphoproteome of Potato Reveals Functionally Distinct and Diverse Canonical and Non-Canonical Proteoforms

National Institute of Plant Genome Research, Aruna Asaf Ali Marg, New Delhi 110067, India
Present Address: Agricultural Research Center (ARC), Plant Pathology Research Institute, 9 Gamaa st., Giza 12619, Egypt
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Author to whom correspondence should be addressed.
Academic Editors: Nicolas L. Taylor and Jacek R. Wisniewski
Received: 27 March 2016 / Revised: 6 June 2016 / Accepted: 13 June 2016 / Published: 24 June 2016
(This article belongs to the Special Issue Sub-cellular Proteomics)
View Full-Text   |   Download PDF [1980 KB, uploaded 24 June 2016]   |  

Abstract

The extracellular matrix (ECM) has a molecular machinery composed of diverse proteins and proteoforms that combine properties of tensile strength with extensibility exhibiting growth-regulatory functions and self- and non-self-recognition. The identification of ECM proteoforms is the prerequisite towards a comprehensive understanding of biological functions accomplished by the outermost layer of the cell. Regulatory mechanisms of protein functions rely on post-translational modifications, phosphorylation in particular, affecting enzymatic activity, interaction, localization and stability. To investigate the ECM proteoforms, we have isolated the cell wall proteome and phosphoproteome of a tuberous crop, potato (Solanum tuberosum). LC-MS/MS analysis led to the identification of 38 proteins and 35 phosphoproteins of known and unknown functions. The findings may provide a better understanding of biochemical machinery and the integrated protein and phosphoprotein network of ECM for future functional studies of different developmental pathways and guidance cues in mechanosensing and integrity signaling. View Full-Text
Keywords: ECM; proteoforms; phosphoproteome; potato; 2-DE; protein interaction network ECM; proteoforms; phosphoproteome; potato; 2-DE; protein interaction network
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Elagamey, E.; Narula, K.; Sinha, A.; Aggarwal, P.R.; Ghosh, S.; Chakraborty, N.; Chakraborty, S. Extracellular Matrix Proteome and Phosphoproteome of Potato Reveals Functionally Distinct and Diverse Canonical and Non-Canonical Proteoforms. Proteomes 2016, 4, 20.

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