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Proteomes 2016, 4(3), 21; doi:10.3390/proteomes4030021

Understanding the Remodelling of Cell Walls during Brachypodium distachyon Grain Development through a Sub-Cellular Quantitative Proteomic Approach

1
UR1268 BIA (Biopolymères Interactions Assemblages), INRA, Nantes 44300, France
2
Laboratoire de Recherche en Sciences Végétales, Université de Toulouse, CNRS, UPS, 24 Chemin de Borderouge-Auzeville, BP42617, Castanet-Tolosan 31326, France
*
Author to whom correspondence should be addressed.
Academic Editors: Nicolas L. Taylor and Jacek R. Wisniewski
Received: 28 April 2016 / Revised: 16 June 2016 / Accepted: 20 June 2016 / Published: 24 June 2016
(This article belongs to the Special Issue Sub-cellular Proteomics)
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Abstract

Brachypodium distachyon is a suitable plant model for studying temperate cereal crops, such as wheat, barley or rice, and helpful in the study of the grain cell wall. Indeed, the most abundant hemicelluloses that are in the B. distachyon cell wall of grain are (1-3)(1-4)-β-glucans and arabinoxylans, in a ratio similar to those of cereals such as barley or oat. Conversely, these cell walls contain few pectins and xyloglucans. Cell walls play an important role in grain physiology. The modifications of cell wall polysaccharides that occur during grain development and filling are key in the determination of the size and weight of the cereal grains. The mechanisms required for cell wall assembly and remodelling are poorly understood, especially in cereals. To provide a better understanding of these processes, we purified the cell wall at three developmental stages of the B. distachyon grain. The proteins were then extracted, and a quantitative and comparative LC-MS/MS analysis was performed to investigate the protein profile changes during grain development. Over 466 cell wall proteins (CWPs) were identified and classified according to their predicted functions. This work highlights the different proteome profiles that we could relate to the main phases of grain development and to the reorganization of cell wall polysaccharides that occurs during these different developmental stages. These results provide a good springboard to pursue functional validation to better understand the role of CWPs in the assembly and remodelling of the grain cell wall of cereals. View Full-Text
Keywords: Brachypodium distachyon; cell wall; cell wall proteins; grain development; grass; quantitative mass spectrometry; Pooideae; polysaccharide remodelling; proteomics Brachypodium distachyon; cell wall; cell wall proteins; grain development; grass; quantitative mass spectrometry; Pooideae; polysaccharide remodelling; proteomics
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Francin-Allami, M.; Lollier, V.; Pavlovic, M.; San Clemente, H.; Rogniaux, H.; Jamet, E.; Guillon, F.; Larré, C. Understanding the Remodelling of Cell Walls during Brachypodium distachyon Grain Development through a Sub-Cellular Quantitative Proteomic Approach. Proteomes 2016, 4, 21.

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